##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546493_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	977175
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38240335661473	31.0	31.0	33.0	30.0	34.0
2	31.782884334945123	31.0	31.0	34.0	30.0	34.0
3	31.921295571417605	31.0	31.0	34.0	30.0	34.0
4	35.61825261595927	37.0	35.0	37.0	33.0	37.0
5	35.51917005654054	37.0	35.0	37.0	33.0	37.0
6	35.549033694067084	37.0	35.0	37.0	33.0	37.0
7	35.9600061401489	37.0	35.0	37.0	35.0	37.0
8	35.942932432778164	37.0	35.0	37.0	35.0	37.0
9	37.64557525519994	39.0	37.0	39.0	35.0	39.0
10	37.0753406503441	39.0	37.0	39.0	33.0	39.0
11	36.80904239261135	39.0	37.0	39.0	32.0	39.0
12	36.28238544784711	38.0	35.0	39.0	32.0	39.0
13	36.101887584107246	38.0	35.0	39.0	32.0	39.0
14	37.09672883567426	39.0	35.0	41.0	31.0	41.0
15	37.286152429196406	39.0	35.0	41.0	32.0	41.0
16	37.356975976667435	39.0	35.0	41.0	32.0	41.0
17	37.28891549620078	39.0	35.0	41.0	32.0	41.0
18	37.229202548161794	39.0	35.0	41.0	32.0	41.0
19	37.158428121881954	39.0	35.0	41.0	32.0	41.0
20	36.97554992708573	38.0	35.0	41.0	32.0	41.0
21	36.84556502161844	38.0	35.0	41.0	31.0	41.0
22	36.789331491288664	38.0	35.0	41.0	31.0	41.0
23	36.693865479571215	38.0	35.0	41.0	31.0	41.0
24	36.607899301558064	38.0	35.0	41.0	31.0	41.0
25	36.40036431550132	38.0	35.0	40.0	31.0	41.0
26	36.294575690127154	38.0	35.0	40.0	31.0	41.0
27	36.187966331516876	38.0	35.0	40.0	30.0	41.0
28	36.222863867782124	38.0	35.0	40.0	30.0	41.0
29	36.13204697213907	38.0	35.0	40.0	30.0	41.0
30	36.0769053649551	38.0	35.0	40.0	30.0	41.0
31	35.91525775833397	38.0	35.0	40.0	30.0	41.0
32	35.659346585821375	38.0	34.0	40.0	29.0	41.0
33	35.511992222478064	38.0	34.0	40.0	28.0	41.0
34	35.36967175787346	38.0	34.0	40.0	27.0	41.0
35	35.1478230613759	37.0	34.0	40.0	26.0	41.0
36	34.96324711540922	37.0	34.0	40.0	25.0	41.0
37	34.89840714303988	37.0	34.0	40.0	25.0	41.0
38	34.839757464118506	37.0	34.0	40.0	25.0	41.0
39	34.72557832527439	37.0	34.0	40.0	24.0	41.0
40	34.566039859799936	37.0	34.0	40.0	24.0	41.0
41	34.51598331926216	36.0	34.0	40.0	24.0	41.0
42	34.29590707908	36.0	33.0	40.0	23.0	41.0
43	34.29447233095402	36.0	33.0	40.0	23.0	41.0
44	33.9440867807711	35.0	33.0	40.0	23.0	41.0
45	33.86506408780413	35.0	33.0	40.0	23.0	41.0
46	33.87548903727582	35.0	33.0	40.0	23.0	41.0
47	33.76734873487349	35.0	33.0	40.0	23.0	41.0
48	33.70102284647069	35.0	33.0	39.0	23.0	41.0
49	33.66766853429529	35.0	33.0	39.0	23.0	41.0
50	33.12865044643999	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	1.0
13	4.0
14	17.0
15	22.0
16	71.0
17	241.0
18	467.0
19	902.0
20	1658.0
21	2573.0
22	3945.0
23	5713.0
24	8116.0
25	11595.0
26	16079.0
27	19578.0
28	21651.0
29	22793.0
30	26211.0
31	30878.0
32	37537.0
33	48565.0
34	79034.0
35	117900.0
36	79504.0
37	98522.0
38	136806.0
39	206788.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.95236267812828	22.78148745107069	27.127587177322383	16.13856269347865
2	33.525929337119756	24.374856090260188	28.268529178499247	13.830685394120806
3	29.264358994038943	24.892675314043032	31.72840074705145	14.114564944866578
4	25.10880855527413	27.863074679560977	31.689513137360247	15.338603627804643
5	23.16555376467879	31.29429221991967	30.300611456494487	15.239542558907054
6	20.88489779210479	40.00460511167396	27.765548647888043	11.344948448333206
7	84.07409113004324	4.733236114309106	8.828510758052548	2.3641619975951085
8	84.13805101440377	3.932765369560212	8.748893494000564	3.180290122035459
9	78.27420881623046	5.9319978509478855	11.560160667229514	4.233632665592141
10	46.534346457901606	23.849975695243945	16.1096016578402	13.506076189014248
11	37.703891319364494	22.729910200322358	23.068539412080742	16.497659068232405
12	31.926011205771744	20.397779312815004	28.391229820656484	19.284979660756775
13	23.584516591194003	28.112057717399647	29.920843247115407	18.38258244429094
14	19.744160462557883	30.143935323764936	31.139048788599787	18.97285542507739
15	18.92782766648758	20.866068002149053	40.66866221505872	19.537442116304653
16	21.78197354619183	19.34259472458874	37.79896129147799	21.07647043774145
17	21.58692148284596	19.770460767006934	29.801622022667384	28.840995727479722
18	25.392585770204928	20.476168547087266	31.36735999181314	22.763885690894668
19	28.497761404047385	23.270447975030063	26.320464604599998	21.911326016322562
20	29.57760892368307	22.816588635607747	25.52152889707575	22.084273543633433
21	25.645764576457648	25.16693529818098	27.97503006114565	21.212270064215723
22	25.38317087522706	22.202266738301738	27.002532811420675	25.412029575050525
23	23.14969171335738	26.06175966433853	26.11886304909561	24.669685573208483
24	23.186737278379	22.80215928569601	32.24366157545987	21.767441860465116
25	23.135671706705555	22.9900478419935	30.272776114820783	23.601504336480158
26	21.43055235756134	25.70276562539975	28.09834471819275	24.768337298846163
27	22.801442934991172	24.961444981707473	28.317957377133062	23.919154706168293
28	21.338654795712127	25.030214649371914	31.93041164581574	21.70071890910021
29	22.43344334433443	23.488218589300793	31.05114232353468	23.0271957428301
30	23.418220891856627	24.28387955074577	30.257425742574256	22.04047381482334
31	28.032952132422544	24.21910097986543	25.20950699721135	22.538439890500676
32	29.13613221787295	23.923657482027274	25.224959705272852	21.715250594826923
33	26.681710031468263	24.716657712282856	25.119860823291628	23.48177143295725
34	23.13096425921662	25.719344027426	27.616036022206874	23.53365569115051
35	23.233607081638397	24.801494102898662	28.903420574615602	23.06147824084734
36	27.81988896564075	25.34285056412618	24.98559623404201	21.851664236191063
37	23.99969299255507	27.00283981886561	26.423312098651724	22.574155089927597
38	24.71819274950751	27.747025865377235	23.977281449075143	23.557499936040116
39	23.69329956251439	26.467828178166652	24.69772558651214	25.141146672806812
40	26.255890705349604	23.02647939212526	26.16327679279556	24.554353109729576
41	22.21342134213421	23.39611635582163	26.838079156752887	27.552383145291277
42	25.247780592012692	24.550669020390412	24.830557474352087	25.370992913244812
43	24.145521528897078	24.05280528052805	26.880343848338324	24.92132934223655
44	23.22368050758564	26.08632025993297	27.272597027144574	23.417402205336813
45	22.42280041957684	26.575485455522298	26.168905262619287	24.832808862281578
46	24.251746104843043	25.686084887558525	26.225803975746416	23.836365031852022
47	23.706500882646402	25.1942589607798	26.9843170363548	24.114923120218997
48	25.03031698518689	23.558523294189886	27.939621869163663	23.471537851459566
49	24.13334356691483	22.688054851996828	28.298001893212575	24.880599687875765
50	22.076700693325147	25.699081536060582	28.365952874357202	23.85826489625707
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1131.0
1	1321.5
2	1512.0
3	8060.5
4	14609.0
5	9972.0
6	5335.0
7	5492.5
8	5650.0
9	5746.5
10	5843.0
11	5859.0
12	5875.0
13	5592.5
14	5310.0
15	5004.5
16	4699.0
17	4448.5
18	4198.0
19	4063.5
20	3929.0
21	4095.0
22	4261.0
23	4176.0
24	4091.0
25	4301.0
26	4511.0
27	4960.0
28	5409.0
29	6706.0
30	8003.0
31	9034.5
32	10066.0
33	11373.0
34	12680.0
35	15046.0
36	17412.0
37	18953.5
38	20495.0
39	25513.0
40	30531.0
41	38221.5
42	45912.0
43	53297.0
44	60682.0
45	64548.0
46	68414.0
47	71881.5
48	75349.0
49	76956.0
50	78563.0
51	73391.5
52	68220.0
53	65464.0
54	62708.0
55	58152.0
56	53596.0
57	52772.0
58	51948.0
59	51061.5
60	50175.0
61	48339.5
62	46504.0
63	41912.0
64	37320.0
65	33960.0
66	30600.0
67	26703.5
68	22807.0
69	20013.0
70	17219.0
71	14872.0
72	12525.0
73	10314.0
74	8103.0
75	6559.5
76	5016.0
77	3985.5
78	2955.0
79	2299.0
80	1643.0
81	1220.0
82	797.0
83	607.0
84	417.0
85	257.0
86	97.0
87	60.5
88	24.0
89	23.5
90	23.0
91	14.0
92	5.0
93	3.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	977175.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.060513244146204
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.94048030031922	24.208713206231813
2	6.1896197655057215	3.8450553340543916
3	2.419526430846308	2.254551982495906
4	1.503499522993288	1.8679786738600206
5	1.073218182609453	1.6667353787399715
6	0.8571478254175816	1.5974070830144345
7	0.7332428586959596	1.5942429666591034
8	0.6345183282311172	1.5766771950140899
9	0.5699542862161541	1.5932765390017274
>10	7.861580475376903	53.38413434365048
>50	0.18778758706085844	3.525236673695083
>100	0.02744076683562262	1.5148780842265028
>500	0.0013224465944872163	0.27038191402977707
>1k	3.306116486218041E-4	0.14725699381644522
>5k	3.306116486218041E-4	0.9534736315102469
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9285	0.9501880420600199	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1434	0.14674955867679793	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06590426484508916	0.0
2	0.0	0.0	0.0	0.19607542149563795	0.0
3	0.0	0.0	0.0	0.3223578171770665	0.0
4	0.0	0.0	0.0	0.45723642131655023	0.0
5	0.0	0.0	0.0	0.730780054749661	0.0
6	0.0	0.0	0.0	1.3186993117916443	0.0
7	0.0	0.0	0.0	1.690280655972574	0.0
8	0.0	0.0	0.0	2.663084913142477	0.0
9	0.0	0.0	0.0	3.2221454703609895	0.0
10	0.0	0.0	0.0	4.01330365594699	0.0
11	0.0	0.0	0.0	4.501138485941618	0.0
12	0.0	0.0	0.0	4.9792514135134445	0.0
13	0.0	0.0	0.0	5.2562744646557675	0.0
14	0.0	0.0	0.0	5.397497889323816	0.0
15	0.0	0.0	0.0	5.511602323023	0.0
16	0.0	0.0	0.0	5.698262849540768	0.0
17	0.0	0.0	0.0	5.8908588533271935	0.0
18	0.0	0.0	0.0	6.230716094865301	0.0
19	0.0	0.0	0.0	6.363036303630363	0.0
20	0.0	0.0	0.0	6.514493309796096	0.0
21	0.0	0.0	0.0	6.6689180545961575	0.0
22	0.0	0.0	0.0	6.824673164990918	0.0
23	0.0	0.0	0.0	7.001611789085885	0.0
24	0.0	0.0	0.0	7.151329086397012	0.0
25	0.0	0.0	0.0	7.274234400184205	0.0
26	0.0	0.0	0.0	7.39703737815642	0.0
27	0.0	0.0	0.0	7.556169570445417	0.0
28	0.0	0.0	0.0	7.689717808990201	0.0
29	0.0	0.0	0.0	7.834113643922532	0.0
30	0.0	0.0	0.0	8.057922071276895	0.0
31	0.0	0.0	0.0	8.214393532376494	0.0
32	0.0	0.0	0.0	8.385294343387827	0.0
33	0.0	0.0	0.0	8.54422186404687	0.0
34	0.0	0.0	0.0	8.696599892547395	0.0
35	0.0	0.0	0.0	8.878348299946273	0.0
36	1.0233581497684652E-4	0.0	0.0	9.031442679151636	0.0
37	1.0233581497684652E-4	0.0	0.0	9.190677207255609	0.0
38	1.0233581497684652E-4	0.0	0.0	9.383682554301942	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGG	90	0.0	44.0	2
TCGATAG	60	0.0	44.0	1
TCGACAG	20	7.8559027E-4	44.0	1
CGTTTTT	4800	0.0	42.304165	1
ATGGTCG	75	0.0	41.066666	44
CGTAAGG	205	0.0	39.707317	2
TATACTA	305	0.0	39.67213	44
GTTGATC	690	0.0	38.89855	16
ACGGGAT	150	0.0	38.13333	5
TATGCTT	695	0.0	37.669067	35
GCCAGTA	715	0.0	36.923077	25
AGGGCGA	1085	0.0	36.903225	6
CATATGC	710	0.0	36.87324	33
TAGGGCG	490	0.0	36.816326	5
TAGCATA	700	0.0	36.771427	30
TCGTTTA	90	0.0	36.666668	38
TACGAAT	30	1.3009527E-4	36.666664	12
GCCCGTT	30	1.3009527E-4	36.666664	44
GTACGGG	355	0.0	36.56338	3
AGGGTAC	500	0.0	36.52	6
>>END_MODULE