FastQCFastQC Report
Thu 26 May 2016
SRR1546492_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546492_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1745979
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258881.4827211552945367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT152700.042.530451
TAACGCC1300.038.92307712
GTTTTTT176800.037.8280532
CGGTCTA700.037.71428731
CATATGC12100.037.09090833
TATAGCG950.037.052631
GCCAGTA11950.037.0041825
AAGTACG301.3013721E-436.6666641
TACGGGA4200.036.6666644
TTGATCC12500.036.60799817
TAGCCGT1750.036.4571444
TAGCATA12400.036.19354630
GGGCGAT37950.036.0579727
AGGGTAC8700.035.9080476
ATATGCT12550.035.76095634
ACGGGAC3200.035.755
GTTGATC12900.035.6434116
GTCTCAA11850.035.45991542
TAGCGGG8750.035.451433
GTAGCAT12750.035.3725529
Produced by FastQC (version 0.11.5)