##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546492_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1745979
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35702720364907	31.0	31.0	33.0	30.0	34.0
2	31.755691219653844	31.0	31.0	34.0	30.0	34.0
3	31.859092234213584	31.0	31.0	34.0	30.0	34.0
4	35.57850638524289	37.0	35.0	37.0	33.0	37.0
5	35.477717085944334	37.0	35.0	37.0	33.0	37.0
6	35.50405417247286	37.0	35.0	37.0	33.0	37.0
7	35.95060364414463	37.0	35.0	37.0	35.0	37.0
8	35.92222586869602	37.0	35.0	37.0	35.0	37.0
9	37.60665219913871	39.0	37.0	39.0	35.0	39.0
10	37.02806162044332	39.0	37.0	39.0	33.0	39.0
11	36.816848885353146	39.0	37.0	39.0	32.0	39.0
12	36.5578526431303	39.0	35.0	39.0	32.0	39.0
13	36.49864975466486	39.0	35.0	39.0	32.0	39.0
14	37.59522422663732	40.0	36.0	41.0	32.0	41.0
15	37.69941562871031	40.0	36.0	41.0	32.0	41.0
16	37.717598550727125	40.0	36.0	41.0	32.0	41.0
17	37.635098131191725	40.0	36.0	41.0	32.0	41.0
18	37.504421301745325	39.0	36.0	41.0	32.0	41.0
19	37.393651928230526	39.0	36.0	41.0	32.0	41.0
20	37.152745250658796	39.0	35.0	41.0	32.0	41.0
21	37.045631705765075	39.0	35.0	41.0	32.0	41.0
22	36.99067113636533	39.0	35.0	41.0	32.0	41.0
23	36.865166190429555	38.0	35.0	41.0	31.0	41.0
24	36.768522989108114	38.0	35.0	41.0	31.0	41.0
25	36.5862854020581	38.0	35.0	41.0	31.0	41.0
26	36.47518269120075	38.0	35.0	40.0	31.0	41.0
27	36.388618648907006	38.0	35.0	40.0	30.0	41.0
28	36.4042408299298	38.0	35.0	40.0	31.0	41.0
29	36.2804014252176	38.0	35.0	40.0	30.0	41.0
30	36.20345490982423	38.0	35.0	40.0	30.0	41.0
31	36.03488873577517	38.0	35.0	40.0	30.0	41.0
32	35.70835216231123	38.0	35.0	40.0	28.0	41.0
33	35.480756641402905	38.0	35.0	40.0	26.0	41.0
34	35.22191446747068	38.0	34.0	40.0	25.0	41.0
35	34.93685720160437	38.0	34.0	40.0	23.0	41.0
36	34.76250057990388	38.0	34.0	40.0	23.0	41.0
37	34.689579313382346	38.0	34.0	40.0	23.0	41.0
38	34.603223750113834	38.0	34.0	40.0	23.0	41.0
39	34.45607364120645	38.0	34.0	40.0	22.0	41.0
40	34.33066434361467	37.0	33.0	40.0	21.0	41.0
41	34.27180223817125	37.0	33.0	40.0	21.0	41.0
42	34.07663837881211	37.0	33.0	40.0	21.0	41.0
43	34.09210362782141	37.0	33.0	40.0	21.0	41.0
44	33.8363388104897	37.0	33.0	40.0	20.0	41.0
45	33.74224947722739	36.0	33.0	40.0	20.0	41.0
46	33.7413416770763	36.0	33.0	40.0	20.0	41.0
47	33.615788047851666	36.0	33.0	40.0	20.0	41.0
48	33.55799869299688	36.0	33.0	40.0	20.0	41.0
49	33.52402405756312	36.0	33.0	40.0	20.0	41.0
50	33.00297769904449	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	6.0
13	11.0
14	20.0
15	64.0
16	143.0
17	370.0
18	868.0
19	1719.0
20	3084.0
21	4903.0
22	7435.0
23	10888.0
24	15623.0
25	23381.0
26	34084.0
27	42134.0
28	44299.0
29	43962.0
30	46400.0
31	53062.0
32	63724.0
33	78748.0
34	119108.0
35	160933.0
36	145501.0
37	180851.0
38	254405.0
39	410248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.63116051223984	21.864581418218663	25.22796665939281	19.27629141014869
2	37.23481210255106	23.494841575986882	25.80947422620776	13.460872095254295
3	28.315460838876067	24.44233292611194	33.565867630710336	13.676338604301655
4	25.065135376771426	26.61807501693892	33.39547611970132	14.921313486588328
5	22.5541086118447	30.251509325140795	32.34861358584496	14.84576847716954
6	19.938613236470772	39.190333904359676	30.234613360183598	10.636439498985956
7	80.59363829690965	4.0859025223098335	12.833774060283657	2.486685120496867
8	79.541277415135	3.9671152974921235	12.772662214150344	3.71894507322253
9	73.43690846224382	5.81415927682979	15.724015008198839	5.024917252727553
10	38.538951499416655	25.74143217071912	21.395789983728328	14.323826346135892
11	29.348233856191857	24.654305693252898	29.453618857958773	16.543841592596475
12	26.483136395111284	20.51634068909191	33.62549034094911	19.37503257484769
13	23.070208748215183	23.39415307973349	34.80981157276233	18.725826599288993
14	19.788725981240322	26.224141298377585	34.24920918292832	19.73792353745377
15	19.009106065995066	21.834455053583117	39.811074474549805	19.345364405872008
16	21.566811513769636	21.16285476514895	37.05897951808126	20.211354203000152
17	21.250885606298816	21.52431386631798	33.48797436853479	23.736826158848416
18	23.166658934614905	21.41091044050358	34.568457008933095	20.853973615948416
19	25.806495954418697	24.592449279172314	29.817540760799528	19.783514005609458
20	26.376949550939617	23.24019933802182	29.457857167812442	20.924993943226124
21	24.489756176907054	25.121722540763663	30.57682824363867	19.811693038690613
22	23.220955120307863	22.424954710222746	30.42195811060729	23.932132058862106
23	21.69355988817735	25.566745075398963	29.959524140897457	22.780170895526236
24	21.416695160709263	23.149018401710446	33.88551637791749	21.548770059662804
25	22.77094970787163	24.070621697053628	31.26612633943478	21.892302255639958
26	20.73707644822761	24.30481695369761	31.686692680725255	23.27141391734952
27	22.0154423392263	23.317634404537515	30.968413709443244	23.698509546792945
28	21.532103192535534	24.746345746426503	32.70159606730665	21.01995499373131
29	22.432801310897784	23.801088100143243	31.61458413875539	22.151526450203583
30	22.542596445890815	24.210829568969615	31.65003702793676	21.596536957202808
31	24.695600577097434	24.092844186556654	28.223649883532392	22.987905352813524
32	25.334382601394406	25.43404015741312	27.620148925044347	21.61142831614813
33	23.961284757720453	24.876301490453205	27.193053295600922	23.96936045622542
34	22.46447408588534	27.139501677855232	28.338198798496432	22.057825437762997
35	22.490763061869586	26.130497560394485	28.23098101409009	23.14775836364584
36	25.80157035107524	27.532461730639373	25.387361474565274	21.278606443720115
37	23.47296273322875	28.538544850768538	26.057300803732463	21.93119161227025
38	25.026990588088406	27.80789459667041	25.824823780812945	21.340291034428247
39	23.16425340740066	27.738248856372273	25.845958055623807	23.251539680603262
40	25.27395804875087	25.106201162786036	28.42153313413277	21.19830765433032
41	21.81727271633851	25.72728537972106	28.50939215191019	23.946049752030234
42	24.283625404429262	25.996532604344036	26.898662584143334	22.82117940708336
43	23.169465383031525	24.686837585102683	28.62628931963099	23.517407712234796
44	23.251539680603262	25.708442083209476	28.79404620559583	22.24597203059143
45	21.75879549524937	25.77344859245157	28.987290225140168	23.480465687158894
46	23.563628199422787	25.840516982163013	28.059558562846405	22.53629625556779
47	22.774557998692995	25.802257644565024	28.852007956567633	22.571176400174345
48	23.0207808914082	24.748350352438376	28.705098973126255	23.525769783027172
49	23.012705192903237	23.991640220185925	28.971367925960163	24.024286660950676
50	21.54355808403194	24.909177029047886	30.396700074857712	23.15056481206246
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1496.0
1	2074.0
2	2652.0
3	22497.0
4	42342.0
5	29081.5
6	15821.0
7	15930.0
8	16039.0
9	16218.5
10	16398.0
11	16041.0
12	15684.0
13	14934.0
14	14184.0
15	13196.5
16	12209.0
17	11490.0
18	10771.0
19	10245.5
20	9720.0
21	9644.5
22	9569.0
23	9858.5
24	10148.0
25	10782.5
26	11417.0
27	12705.5
28	13994.0
29	16139.0
30	18284.0
31	20655.0
32	23026.0
33	26087.5
34	29149.0
35	34118.5
36	39088.0
37	43694.5
38	48301.0
39	54167.5
40	60034.0
41	68692.5
42	77351.0
43	86059.0
44	94767.0
45	105908.5
46	117050.0
47	128487.0
48	139924.0
49	142016.0
50	144108.0
51	130633.5
52	117159.0
53	108449.5
54	99740.0
55	93733.0
56	87726.0
57	87132.5
58	86539.0
59	83916.5
60	81294.0
61	76076.0
62	70858.0
63	65482.5
64	60107.0
65	52160.0
66	44213.0
67	38876.0
68	33539.0
69	29489.0
70	25439.0
71	21518.0
72	17597.0
73	14992.0
74	12387.0
75	9855.5
76	7324.0
77	5851.0
78	4378.0
79	3322.0
80	2266.0
81	1611.0
82	956.0
83	752.5
84	549.0
85	387.5
86	226.0
87	158.5
88	91.0
89	68.0
90	45.0
91	29.5
92	14.0
93	9.0
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1745979.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.875704433461472
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.57825579625516	23.45358734491053
2	6.743184420538867	4.298875070588382
3	3.0555660749577642	2.9219496324679697
4	2.1140726859628076	2.6955022435441784
5	1.6379675658618988	2.610568500050511
6	1.334374636744619	2.5520478914627396
7	1.2022017586660663	2.682471955003897
8	0.9981964747645014	2.5454572636893142
9	0.8909128816893862	2.555862812342487
>10	8.31611411384034	47.658792571381866
>50	0.09879025051507098	2.0019947812161756
>100	0.026748701614160108	1.693043474753473
>500	0.00307244280093619	0.7001867824955594
>1k	3.614638589336694E-4	0.13826298156897668
>5k	0.0	0.0
>10k+	1.807319294668347E-4	1.4913966945240285
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25888	1.4827211552945367	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.047136878507702554	0.0
2	0.0	0.0	0.0	0.1705633343814559	0.0
3	0.0	0.0	0.0	0.3419857856251421	0.0
4	0.0	0.0	0.0	0.5321369844654489	0.0
5	0.0	0.0	0.0	0.9075137788026088	0.0
6	0.0	0.0	0.0	1.7317504964263601	0.0
7	0.0	0.0	0.0	2.28885914435397	0.0
8	0.0	0.0	0.0	3.7885908135206665	0.0
9	0.0	0.0	0.0	4.777835243150118	0.0
10	0.0	0.0	0.0	6.016739032943695	0.0
11	0.0	0.0	0.0	6.546985960312237	0.0
12	0.0	0.0	0.0	7.088687779177183	0.0
13	0.0	0.0	0.0	7.433880934421319	0.0
14	0.0	0.0	0.0	7.606105228069754	0.0
15	0.0	0.0	0.0	7.737721931363436	0.0
16	0.0	0.0	0.0	7.931080499822736	0.0
17	0.0	0.0	0.0	8.115905174117215	0.0
18	0.0	0.0	0.0	8.465852109332358	0.0
19	0.0	0.0	0.0	8.601248926819853	0.0
20	0.0	0.0	0.0	8.766886657857855	0.0
21	0.0	0.0	0.0	8.91047372276528	0.0
22	0.0	0.0	0.0	9.061506467145367	0.0
23	5.7274457482020115E-5	0.0	0.0	9.232986192846536	0.0
24	5.7274457482020115E-5	0.0	0.0	9.375084121859427	0.0
25	5.7274457482020115E-5	0.0	0.0	9.50074428157498	0.0
26	5.7274457482020115E-5	0.0	0.0	9.619130585190314	5.7274457482020115E-5
27	5.7274457482020115E-5	0.0	0.0	9.772225210039755	5.7274457482020115E-5
28	5.7274457482020115E-5	0.0	0.0	9.901436386119192	5.7274457482020115E-5
29	5.7274457482020115E-5	0.0	0.0	10.040785141172947	5.7274457482020115E-5
30	5.7274457482020115E-5	0.0	0.0	10.22858808725649	5.7274457482020115E-5
31	5.7274457482020115E-5	0.0	0.0	10.373549739143483	5.7274457482020115E-5
32	5.7274457482020115E-5	0.0	0.0	10.54187936968314	5.7274457482020115E-5
33	5.7274457482020115E-5	0.0	0.0	10.70654343494395	5.7274457482020115E-5
34	5.7274457482020115E-5	0.0	0.0	10.862673606039936	5.7274457482020115E-5
35	5.7274457482020115E-5	0.0	0.0	11.03747525027506	5.7274457482020115E-5
36	5.7274457482020115E-5	0.0	0.0	11.192975402338746	5.7274457482020115E-5
37	5.7274457482020115E-5	0.0	0.0	11.354031176778186	5.7274457482020115E-5
38	5.7274457482020115E-5	0.0	0.0	11.53673669614583	5.7274457482020115E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15270	0.0	42.53045	1
TAACGCC	130	0.0	38.923077	12
GTTTTTT	17680	0.0	37.828053	2
CGGTCTA	70	0.0	37.714287	31
CATATGC	1210	0.0	37.090908	33
TATAGCG	95	0.0	37.05263	1
GCCAGTA	1195	0.0	37.00418	25
AAGTACG	30	1.3013721E-4	36.666664	1
TACGGGA	420	0.0	36.666664	4
TTGATCC	1250	0.0	36.607998	17
TAGCCGT	175	0.0	36.45714	44
TAGCATA	1240	0.0	36.193546	30
GGGCGAT	3795	0.0	36.057972	7
AGGGTAC	870	0.0	35.908047	6
ATATGCT	1255	0.0	35.760956	34
ACGGGAC	320	0.0	35.75	5
GTTGATC	1290	0.0	35.64341	16
GTCTCAA	1185	0.0	35.459915	42
TAGCGGG	875	0.0	35.45143	3
GTAGCAT	1275	0.0	35.37255	29
>>END_MODULE