FastQCFastQC Report
Thu 26 May 2016
SRR1546491_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546491_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1497004
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174811.1677323507485617No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG502.7284841E-1144.01
CGTTTTT93650.042.4495431
TATAGCG501.3478711E-939.6000021
ATAACGC1900.038.21052611
TCGTTAG750.038.133331
CATATGC16000.037.9533
ATTACCG357.2916846E-637.71428710
GCGATAT1700.037.529419
TATGGGC6450.037.1782954
GTATGCG601.9826984E-1036.6666681
AGGGTAC11000.036.66
TAGCATA16550.036.55589330
CGGTCTA1450.036.4137931
GTTTTTT113150.036.39772
TAAGGGC9500.036.35794
TATACTA7350.036.2176944
CCAGTAG16950.036.2123926
ATCCTGC17050.036.12903220
CGTTAGG2200.036.02
GTAGCAT17050.036.029
Produced by FastQC (version 0.11.5)