##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546491_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1497004
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32527969197143	31.0	31.0	33.0	30.0	34.0
2	31.730838394553388	31.0	31.0	34.0	30.0	34.0
3	31.85737312659151	31.0	31.0	34.0	30.0	34.0
4	35.5643819254992	37.0	35.0	37.0	33.0	37.0
5	35.461012128224105	37.0	35.0	37.0	33.0	37.0
6	35.48452308744666	37.0	35.0	37.0	33.0	37.0
7	35.9434978129651	37.0	35.0	37.0	35.0	37.0
8	35.92460875188042	37.0	35.0	37.0	35.0	37.0
9	37.622085846130005	39.0	37.0	39.0	35.0	39.0
10	36.998327993779576	39.0	37.0	39.0	32.0	39.0
11	36.78679816486796	39.0	37.0	39.0	32.0	39.0
12	36.451758979935924	39.0	35.0	39.0	32.0	39.0
13	36.36302441409642	39.0	35.0	39.0	32.0	39.0
14	37.413883997637946	40.0	36.0	41.0	32.0	41.0
15	37.55751554438064	40.0	36.0	41.0	32.0	41.0
16	37.59701777683961	40.0	36.0	41.0	32.0	41.0
17	37.50609550809484	39.0	35.0	41.0	32.0	41.0
18	37.42256600516765	39.0	36.0	41.0	32.0	41.0
19	37.34181605393172	39.0	36.0	41.0	32.0	41.0
20	37.142837293687926	39.0	35.0	41.0	32.0	41.0
21	37.03459576594318	39.0	35.0	41.0	32.0	41.0
22	36.97858188755675	39.0	35.0	41.0	31.0	41.0
23	36.866809975123644	38.0	35.0	41.0	31.0	41.0
24	36.78088234901176	38.0	35.0	41.0	31.0	41.0
25	36.57538590411248	38.0	35.0	41.0	31.0	41.0
26	36.46351579554898	38.0	35.0	40.0	31.0	41.0
27	36.37247662664896	38.0	35.0	40.0	30.0	41.0
28	36.403360311662496	38.0	35.0	40.0	30.0	41.0
29	36.27666392340969	38.0	35.0	40.0	30.0	41.0
30	36.22900740412183	38.0	35.0	40.0	30.0	41.0
31	36.105174067671165	38.0	35.0	40.0	30.0	41.0
32	35.8489349393856	38.0	35.0	40.0	29.0	41.0
33	35.70819583648407	38.0	35.0	40.0	28.0	41.0
34	35.53346751244486	38.0	35.0	40.0	27.0	41.0
35	35.29387763826951	38.0	34.0	40.0	26.0	41.0
36	35.11621879433856	38.0	34.0	40.0	25.0	41.0
37	35.06452821769347	38.0	34.0	40.0	25.0	41.0
38	34.971466342107306	38.0	34.0	40.0	25.0	41.0
39	34.840920932742996	38.0	34.0	40.0	24.0	41.0
40	34.707191163149865	37.0	34.0	40.0	24.0	41.0
41	34.65381655626839	37.0	34.0	40.0	23.0	41.0
42	34.44849579560242	37.0	33.0	40.0	23.0	41.0
43	34.45162404375673	37.0	33.0	40.0	23.0	41.0
44	34.1819039895685	36.0	33.0	40.0	23.0	41.0
45	34.07934113736503	36.0	33.0	40.0	23.0	41.0
46	34.065544915043645	36.0	33.0	40.0	23.0	41.0
47	33.944757662638175	36.0	33.0	40.0	23.0	41.0
48	33.85608121287585	36.0	33.0	40.0	23.0	41.0
49	33.81978204467055	35.0	33.0	40.0	23.0	41.0
50	33.30212744922525	35.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	5.0
13	8.0
14	19.0
15	54.0
16	133.0
17	328.0
18	698.0
19	1445.0
20	2556.0
21	3847.0
22	5881.0
23	8519.0
24	12132.0
25	17765.0
26	24824.0
27	30724.0
28	33656.0
29	35463.0
30	39452.0
31	46251.0
32	55658.0
33	70703.0
34	108064.0
35	148853.0
36	125272.0
37	160126.0
38	221395.0
39	343171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.785881667650855	22.188451066263013	26.007946538553	18.017720727533128
2	35.547734007390765	23.99679626774544	27.26111620276232	13.194353522101476
3	29.508070786718005	24.72525123513364	32.286553676543285	13.480124301605073
4	25.88503437532565	26.75089712519138	31.935920010901775	15.428148488581192
5	22.298270412103108	31.01280958501113	31.330911607450613	15.35800839543515
6	19.708163772441488	39.36896628198722	29.624503341340436	11.29836660423085
7	83.2037188945387	4.126709080269659	10.26630523365335	2.4032667915382993
8	82.83371320317113	3.5651207344803355	9.935444394270156	3.6657216680783753
9	76.91081653756436	5.223499736807651	13.308514873707752	4.557168851920236
10	40.00209752278551	23.404813881592833	19.82533112803974	16.767757467581916
11	32.23558520885716	24.274551036603775	26.579020496939222	16.910843257599847
12	28.599990380787226	20.378035062030563	30.6423362930226	20.379638264159613
13	23.90461214532493	24.10461161092422	32.011136910789816	19.979639332961035
14	20.434948737611926	27.408811198901272	32.15288669903353	20.003353364453268
15	19.249380763177655	21.944363542114782	38.34264971903883	20.463605975668735
16	22.015505636591485	20.24590448656116	36.1824684503181	21.556121426529256
17	21.65718996074827	21.00435269378038	31.871123924852572	25.467333420618782
18	23.897865336365168	21.788318534887015	32.37653339603635	21.93728273271147
19	27.104603594913574	25.081830108670385	27.123307619752516	20.69025867666352
20	27.364856740529753	24.189314123409154	26.649494590528818	21.796334545532275
21	24.59372186046263	25.90307039927749	28.707805723966	20.795402016293878
22	24.089581590964354	22.098137346326396	28.479015420132477	25.333265642576773
23	22.388116531418753	25.801333864171372	27.343213511787546	24.46733609262233
24	22.662731696107695	23.84936847196133	31.77366259542393	21.71423723650705
25	24.23260058089357	23.817905630178675	28.86812593687124	23.08136785205651
26	21.406489227817694	25.292985189084334	28.818226270604484	24.482299312493485
27	22.777694648778493	23.255315283058696	29.063382596172087	24.903607471990725
28	22.62138244119588	24.534069381244137	30.799450101669734	22.045098075890245
29	22.84322553580351	23.433738320004487	30.16110845395203	23.561927690239973
30	23.63173378294246	22.965269297877626	30.340132691696216	23.062864227483697
31	25.74007818282383	23.16273036010592	26.387905443138425	24.709286013931827
32	26.086570242965283	23.051575012491615	26.29031051353236	24.57154423101074
33	24.723447632738456	23.09305786758085	26.14441911978859	26.039075379892108
34	22.135478595915576	24.625785903043678	28.357372458590625	24.88136304245012
35	23.08677865924206	23.142690333492762	29.649820574961723	24.120710432303454
36	25.35738047460127	24.016635894092467	27.317161477190442	23.30882215411582
37	23.7227823038549	25.73774017971896	27.906271459528497	22.63320605689764
38	24.50721574558251	25.66158807858897	27.465791674571342	22.365404501257178
39	23.36299702606005	25.431528573069944	26.472273955179816	24.733200445690194
40	24.354377142612847	23.594258933175862	29.540402029653894	22.510961894557397
41	22.20688789074712	23.810958420952783	28.97286847596934	25.009285212330763
42	24.462793686590015	25.6617884788551	26.056176202602	23.819241631952888
43	23.065603031120826	24.333869515378716	28.477479018092133	24.12304843540832
44	23.763196357524762	25.199531864978315	27.752030054695915	23.285241722801008
45	21.948304747348704	25.555509537716663	28.476009416140503	24.020176298794123
46	24.530996577163457	24.75110286946461	27.737801635800572	22.98009891757136
47	23.559389286868974	25.310286412060357	27.52050094722526	23.609823353845414
48	23.470545168883987	24.32124429861243	27.999056782747406	24.20915374975618
49	24.125720438956744	23.06333182810467	27.79144210703512	25.01950562590347
50	22.369278906402386	23.861325687840512	29.36952740273239	24.399868003024707
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1463.0
1	1729.5
2	1996.0
3	14608.5
4	27221.0
5	18480.0
6	9739.0
7	9798.5
8	9858.0
9	9974.5
10	10091.0
11	9936.0
12	9781.0
13	9537.5
14	9294.0
15	8732.0
16	8170.0
17	7722.5
18	7275.0
19	6981.0
20	6687.0
21	6604.5
22	6522.0
23	7017.0
24	7512.0
25	7702.0
26	7892.0
27	8957.0
28	10022.0
29	11546.5
30	13071.0
31	14685.5
32	16300.0
33	19061.0
34	21822.0
35	25160.0
36	28498.0
37	31927.5
38	35357.0
39	40544.0
40	45731.0
41	55475.0
42	65219.0
43	73169.0
44	81119.0
45	90912.0
46	100705.0
47	112144.0
48	123583.0
49	126109.5
50	128636.0
51	117236.5
52	105837.0
53	98238.0
54	90639.0
55	86228.5
56	81818.0
57	81829.5
58	81841.0
59	77830.5
60	73820.0
61	70276.0
62	66732.0
63	62006.0
64	57280.0
65	49187.0
66	41094.0
67	36329.5
68	31565.0
69	29083.0
70	26601.0
71	22598.5
72	18596.0
73	15287.0
74	11978.0
75	9707.5
76	7437.0
77	5843.0
78	4249.0
79	3161.5
80	2074.0
81	1515.5
82	957.0
83	727.5
84	498.0
85	381.0
86	264.0
87	185.5
88	107.0
89	70.0
90	33.0
91	22.5
92	12.0
93	10.0
94	8.0
95	4.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1497004.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.14794058522495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.081660083474	21.88475767153156
2	6.651158565649785	3.8773514938893987
3	2.8000881088603062	2.448504054913653
4	1.8084887753412877	2.1085489349077635
5	1.3092601541995659	1.9081118592605701
6	1.0914247891276025	1.9087670944039816
7	0.9105283677093213	1.8578018734207211
8	0.7678574025984227	1.7905169559091185
9	0.7022714668473474	1.842279029132873
>10	8.630423966716615	52.736638372987386
>50	0.21251451307650213	3.828161959815795
>100	0.029255862612100253	1.6633287660192133
>500	0.004376860543460799	0.8041346408113863
>1k	4.6072216246955766E-4	0.16732661363576942
>5k	0.0	0.0
>10k+	2.3036108123477883E-4	1.1737706793607086
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17481	1.1677323507485617	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.680008871051781E-5	0.0	0.0	0.0431528573069945	0.0
2	6.680008871051781E-5	0.0	0.0	0.17187662825216232	0.0
3	6.680008871051781E-5	0.0	0.0	0.33560364568164147	0.0
4	6.680008871051781E-5	0.0	0.0	0.5255162978856436	0.0
5	6.680008871051781E-5	0.0	0.0	0.8866375774547028	0.0
6	6.680008871051781E-5	0.0	0.0	1.7944507830306398	0.0
7	6.680008871051781E-5	0.0	0.0	2.360915535295831	0.0
8	6.680008871051781E-5	0.0	0.0	3.9502232458964706	0.0
9	1.3360017742103562E-4	0.0	0.0	4.848484038786803	0.0
10	1.3360017742103562E-4	0.0	0.0	5.948681499849032	0.0
11	1.3360017742103562E-4	0.0	0.0	6.455694173161862	0.0
12	1.3360017742103562E-4	0.0	0.0	6.931845205490433	0.0
13	1.3360017742103562E-4	0.0	0.0	7.234382807260368	0.0
14	2.0040026613155342E-4	0.0	0.0	7.398577425310821	0.0
15	2.0040026613155342E-4	0.0	0.0	7.505056766715386	0.0
16	2.0040026613155342E-4	0.0	0.0	7.671455787693286	0.0
17	2.0040026613155342E-4	0.0	0.0	7.831041199622713	0.0
18	2.0040026613155342E-4	0.0	0.0	8.14146121186049	0.0
19	2.0040026613155342E-4	0.0	0.0	8.256958565240975	0.0
20	2.0040026613155342E-4	0.0	0.0	8.404787161557351	0.0
21	2.0040026613155342E-4	0.0	0.0	8.536450136405781	0.0
22	2.0040026613155342E-4	0.0	0.0	8.67686392287529	0.0
23	2.0040026613155342E-4	0.0	0.0	8.84199374216769	0.0
24	2.0040026613155342E-4	0.0	0.0	8.97626192047583	0.0
25	2.0040026613155342E-4	0.0	0.0	9.092961675453106	0.0
26	2.0040026613155342E-4	0.0	0.0	9.208325428656169	0.0
27	2.0040026613155342E-4	0.0	0.0	9.347136012996625	0.0
28	2.6720035484207124E-4	0.0	0.0	9.469313375248163	0.0
29	2.6720035484207124E-4	0.0	0.0	9.598504746814303	0.0
30	2.6720035484207124E-4	0.0	0.0	9.787415397687647	0.0
31	2.6720035484207124E-4	0.0	0.0	9.918877972269946	0.0
32	2.6720035484207124E-4	0.0	0.0	10.081068587659084	0.0
33	2.6720035484207124E-4	0.0	0.0	10.22629198051575	0.0
34	2.6720035484207124E-4	0.0	0.0	10.365436565299758	0.0
35	2.6720035484207124E-4	0.0	0.0	10.536043991866421	0.0
36	2.6720035484207124E-4	0.0	0.0	10.677326179489166	0.0
37	2.6720035484207124E-4	0.0	0.0	10.833771987249198	0.0
38	2.6720035484207124E-4	0.0	0.0	11.017539031291834	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	50	2.7284841E-11	44.0	1
CGTTTTT	9365	0.0	42.449543	1
TATAGCG	50	1.3478711E-9	39.600002	1
ATAACGC	190	0.0	38.210526	11
TCGTTAG	75	0.0	38.13333	1
CATATGC	1600	0.0	37.95	33
ATTACCG	35	7.2916846E-6	37.714287	10
GCGATAT	170	0.0	37.52941	9
TATGGGC	645	0.0	37.178295	4
GTATGCG	60	1.9826984E-10	36.666668	1
AGGGTAC	1100	0.0	36.6	6
TAGCATA	1655	0.0	36.555893	30
CGGTCTA	145	0.0	36.41379	31
GTTTTTT	11315	0.0	36.3977	2
TAAGGGC	950	0.0	36.3579	4
TATACTA	735	0.0	36.21769	44
CCAGTAG	1695	0.0	36.21239	26
ATCCTGC	1705	0.0	36.129032	20
CGTTAGG	220	0.0	36.0	2
GTAGCAT	1705	0.0	36.0	29
>>END_MODULE