FastQCFastQC Report
Thu 26 May 2016
SRR1546490_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546490_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1789924
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139070.7769603625628798No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA24690.1379388175140397No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCCG207.85766E-444.01
CGTTTTT86050.041.9802441
CTAGACG700.040.8571431
TCGTTAG603.6379788E-1240.3333321
GCGATCG1550.038.3225829
AGGGCGA19450.038.2313586
GGGCGAT39800.037.6432157
CATACGA1950.037.2307718
AGTACGG2750.036.8000032
CGGGATA4550.036.7472536
TAACGCC900.036.66666812
GTTTGCG1200.036.6666641
CGTAAGG2400.036.6666642
CGCAATC1200.036.66666420
ACGTAGG2950.036.5423742
TTACGGG5500.036.4000053
ACGGGAT6250.036.2559975
TAGGGCG7650.036.2352945
GGCGATT9900.036.222228
GTTTTTT104950.035.992382
Produced by FastQC (version 0.11.5)