FastQCFastQC Report
Thu 26 May 2016
SRR1546489_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546489_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1272580
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117800.92567854280281No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13830.1086768611796508No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAT1500.042.5333339
CGTTTTT61300.042.097881
CGTAAGG950.039.368422
GCGTAAG452.3512257E-839.111111
TACGGGA2950.038.779664
ATACCGG404.124995E-738.52
TACGGGT1100.038.04
CGACGGT1050.037.71428728
GCGCGAC5750.037.4956559
GGCGATA6250.037.3119968
TAGCCGT950.037.0526344
GCGATAC950.037.052639
CGTATAT301.3011739E-436.66666430
TTATACG301.3011739E-436.6666641
ACCCGCT5650.036.60177234
GGGCGAT27500.036.47
TGGGCGA7650.036.2352946
AGGGATT10150.036.1970446
TCACGAC1100.036.025
CGGTAGT4650.035.9569912
Produced by FastQC (version 0.11.5)