FastQCFastQC Report
Thu 26 May 2016
SRR1546488_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546488_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1153215
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177791.541689971080848No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG351.4460966E-744.01
CGTTTTT93450.042.6345631
ATAACGC1200.038.511
GCGTAAG1100.038.01
GTTTTTT108200.037.9815182
CGATTCG357.2902276E-637.71428710
ACGGGTA1000.037.3999985
CGCATCG1200.036.66666421
TAACGGG2650.036.52833
ACTAGGG5250.035.6190453
ATAGGGC7050.035.574474
GACCGAT6950.035.4532369
TATACTA5500.035.244
CATATGC7650.035.0849733
AGGGATT7600.035.0263186
GGGCGAT25800.034.7906957
CTACGGG1900.034.7368433
AAGGGAC9450.034.687835
AGGGCGA13900.034.661876
CGAATAT702.7284841E-1134.5714314
Produced by FastQC (version 0.11.5)