##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546488_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1153215
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47305576150154	31.0	31.0	33.0	30.0	34.0
2	31.86841742433111	31.0	31.0	34.0	30.0	34.0
3	31.96958589681889	33.0	31.0	34.0	30.0	34.0
4	35.67769409867197	37.0	35.0	37.0	33.0	37.0
5	35.588845965409746	37.0	35.0	37.0	33.0	37.0
6	35.61717459450319	37.0	35.0	37.0	33.0	37.0
7	36.029777621692396	37.0	35.0	37.0	35.0	37.0
8	36.01980203171134	37.0	35.0	37.0	35.0	37.0
9	37.73625473133804	39.0	38.0	39.0	35.0	39.0
10	37.16581643492324	39.0	37.0	39.0	33.0	39.0
11	36.94989139059065	39.0	37.0	39.0	33.0	39.0
12	36.61429135070217	39.0	35.0	39.0	32.0	39.0
13	36.53740976314044	39.0	35.0	39.0	32.0	39.0
14	37.632834293691985	40.0	36.0	41.0	33.0	41.0
15	37.73887869998222	40.0	36.0	41.0	33.0	41.0
16	37.755787082200634	40.0	36.0	41.0	33.0	41.0
17	37.67211578066536	40.0	36.0	41.0	33.0	41.0
18	37.52833253122792	39.0	36.0	41.0	32.0	41.0
19	37.422935879259285	39.0	36.0	41.0	32.0	41.0
20	37.21090169656135	39.0	35.0	41.0	32.0	41.0
21	37.114140901739916	39.0	35.0	41.0	32.0	41.0
22	37.04729560402874	39.0	35.0	41.0	32.0	41.0
23	36.95273821446998	38.0	35.0	41.0	32.0	41.0
24	36.859917708319784	38.0	35.0	41.0	32.0	41.0
25	36.69172010423035	38.0	35.0	41.0	31.0	41.0
26	36.57987279041636	38.0	35.0	40.0	31.0	41.0
27	36.50009235051573	38.0	35.0	40.0	31.0	41.0
28	36.517318973478496	38.0	35.0	40.0	31.0	41.0
29	36.40596246146642	38.0	35.0	40.0	31.0	41.0
30	36.325555945769004	38.0	35.0	40.0	31.0	41.0
31	36.160599714710614	38.0	35.0	40.0	30.0	41.0
32	35.877007323005685	38.0	35.0	40.0	29.0	41.0
33	35.66413634924971	38.0	35.0	40.0	28.0	41.0
34	35.38060899311924	38.0	35.0	40.0	26.0	41.0
35	35.116188221623894	38.0	34.0	40.0	24.0	41.0
36	34.91615006742021	38.0	34.0	40.0	24.0	41.0
37	34.88381611408107	38.0	34.0	40.0	24.0	41.0
38	34.78536266004171	38.0	34.0	40.0	23.0	41.0
39	34.671262513928454	37.0	34.0	40.0	23.0	41.0
40	34.51789909080267	37.0	34.0	40.0	23.0	41.0
41	34.47208369644862	37.0	34.0	40.0	23.0	41.0
42	34.28719449538898	37.0	33.0	40.0	22.0	41.0
43	34.2864825726339	37.0	33.0	40.0	23.0	41.0
44	34.030039498272224	36.0	33.0	40.0	22.0	41.0
45	33.94301409537684	36.0	33.0	40.0	22.0	41.0
46	33.90495787862628	36.0	33.0	40.0	22.0	41.0
47	33.807572742290034	36.0	33.0	40.0	22.0	41.0
48	33.72867331763808	36.0	33.0	40.0	22.0	41.0
49	33.6991098797709	35.0	33.0	40.0	22.0	41.0
50	33.218529935874926	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	4.0
13	11.0
14	8.0
15	33.0
16	85.0
17	190.0
18	454.0
19	933.0
20	1654.0
21	2677.0
22	4150.0
23	6038.0
24	8920.0
25	13572.0
26	20515.0
27	25905.0
28	27422.0
29	27380.0
30	29741.0
31	33855.0
32	41175.0
33	52226.0
34	82877.0
35	115919.0
36	96459.0
37	120905.0
38	171075.0
39	269031.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.36056156050693	21.797496563953818	25.60363852360575	19.23830335193351
2	37.31220977874898	23.363553196932056	26.161557038366652	13.162679985952316
3	28.610710058401946	24.241186595734536	33.5524598622113	13.595643483652225
4	25.345577364151524	26.609695503440385	33.116374656937346	14.92835247547075
5	22.302172621757435	30.430839002267575	32.27637517722194	14.99061319875305
6	19.707513343132028	39.00721027735505	30.684651170857123	10.600625208655803
7	82.18831700940414	3.8587774179142658	11.786787372692862	2.1661181999887273
8	81.92878171026217	3.4203509319597822	11.720451086744449	2.9304162710335886
9	76.50784979383722	5.000888819517609	14.283199576835196	4.2080618098099665
10	40.763170787754234	24.973140307748338	19.932016146165285	14.331672758332143
11	31.901770268336783	24.41027908932853	27.43070459541369	16.257246046920997
12	28.620942322116864	20.80574741050021	31.59029322372671	18.983017043656215
13	24.079638228777807	24.15351864136349	32.731710912535824	19.035132217322875
14	20.56355493121404	26.78945383124569	33.42698456055462	19.220006676985644
15	19.833422215285093	21.722315439878948	39.00695013505721	19.43731220977875
16	22.44039489600812	20.71955359581691	36.93127474061645	19.90877676755852
17	22.42738778111627	20.897317499338804	32.57250382625963	24.102790893285295
18	24.104611889370155	21.67158769180075	33.436609825574585	20.787190593254508
19	27.067979518129746	24.156120064341863	28.95947416570197	19.816426251826417
20	28.040651569741982	23.260796989286472	28.477170345512327	20.221381095459215
21	25.71489271298067	25.01589035869287	29.81074647832364	19.458470450002817
22	24.434038752530967	22.634547764293735	29.686658602255434	23.244754880919864
23	23.149369371712993	25.03123875426525	29.014537618744118	22.804854255277636
24	22.91662872924823	22.89815862610181	33.06425948327068	21.120953161379276
25	23.677544950421215	23.35028593974237	30.669476203483303	22.30269290635311
26	21.245734750241716	24.964295469621884	30.365629999609787	23.424339780526616
27	22.107933039372536	23.31690101151997	30.99638835776503	23.578777591342465
28	22.241212609964318	24.408284665045112	32.45994892539553	20.890553799595047
29	23.17815845267361	23.280914660319194	31.870986763092745	21.669940123914447
30	23.781862011853818	23.1571736406481	32.18549880117757	20.875465546320505
31	26.61559206219135	23.07878409489991	28.526337239803507	21.779286603105234
32	26.773238294680525	23.372918319654186	28.75786388487836	21.095979500786928
33	26.73655823068552	23.489288640886567	27.881531197565067	21.89262193086285
34	23.23417576080783	25.094886903136015	30.12248366523155	21.548453670824607
35	24.842375446035646	24.389467705501577	29.556587453336974	21.2115693951258
36	25.769956166022816	25.443390868138206	27.51499069991285	21.27166226592613
37	24.994905546667358	26.06070854090521	28.07256235827664	20.871823554150787
38	24.43629331911222	26.33654609071162	27.9259288163959	21.30123177378026
39	24.09472648205235	24.915041861231426	27.850747692321033	23.13948396439519
40	24.826420051768316	23.648582441262036	29.9206132421101	21.604384264859544
41	22.12857099500093	23.79252784606513	29.908299840012486	24.17060131892145
42	24.218987786319115	25.296583898058906	27.154953759706558	23.329474555915418
43	23.147201519231018	24.41955749795138	28.486795610532294	23.946445372285307
44	23.355141929301997	25.12150813161466	28.660570665487356	22.86277927359599
45	22.560667351708048	25.833344172595744	28.509081134046994	23.096907341649214
46	23.822530924415656	25.652024991003415	28.01897304492224	22.506471039658692
47	23.162896771200515	25.408965370724452	28.91065412780791	22.517483730267124
48	23.49475162914114	24.109467878929774	29.54765590111124	22.848124590817847
49	23.60175682765139	23.760443629331913	28.951236326270468	23.686563216746226
50	22.01081324818009	24.789393131376197	30.045394830972544	23.154398789471173
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	915.0
1	1334.5
2	1754.0
3	15069.5
4	28385.0
5	19280.0
6	10175.0
7	10104.5
8	10034.0
9	10066.5
10	10099.0
11	9821.5
12	9544.0
13	9154.0
14	8764.0
15	8255.0
16	7746.0
17	7112.5
18	6479.0
19	6206.5
20	5934.0
21	5814.5
22	5695.0
23	5766.5
24	5838.0
25	6296.0
26	6754.0
27	7710.5
28	8667.0
29	10053.0
30	11439.0
31	13202.5
32	14966.0
33	16903.0
34	18840.0
35	22188.5
36	25537.0
37	27300.0
38	29063.0
39	33408.0
40	37753.0
41	44335.0
42	50917.0
43	56809.0
44	62701.0
45	67335.0
46	71969.0
47	78112.5
48	84256.0
49	87052.5
50	89849.0
51	83497.5
52	77146.0
53	71937.0
54	66728.0
55	63269.5
56	59811.0
57	60502.5
58	61194.0
59	60335.5
60	59477.0
61	56299.5
62	53122.0
63	48326.0
64	43530.0
65	38047.5
66	32565.0
67	28043.0
68	23521.0
69	20853.5
70	18186.0
71	15867.5
72	13549.0
73	11098.5
74	8648.0
75	7091.5
76	5535.0
77	4302.0
78	3069.0
79	2384.0
80	1699.0
81	1232.5
82	766.0
83	535.5
84	305.0
85	243.0
86	181.0
87	124.0
88	67.0
89	48.5
90	30.0
91	19.5
92	9.0
93	5.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1153215.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.284174605976666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.01820533574417	24.005163773277808
2	7.215396783360832	4.947478463442903
3	3.895332377082181	4.006447660925989
4	2.8533229180075357	3.9129528451282076
5	2.2485047097954425	3.854406403649392
6	1.9372669678555499	3.98505593906104
7	1.652317017437428	3.965382759117596
8	1.449824107348673	3.9764818275436937
9	1.2985856145292378	4.006884235439691
>10	7.362185562378998	38.80455143220383
>50	0.04443449526166666	1.0090644170467318
>100	0.022085543199968076	1.4396080894931031
>500	0.0022847111984946786	0.5391676291810533
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	2.538567998327421E-4	1.5473545244890954
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17779	1.541689971080848	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04838646739766652	0.0
2	0.0	0.0	0.0	0.16527707322572113	0.0
3	0.0	0.0	0.0	0.33931227047861845	0.0
4	0.0	0.0	0.0	0.4726785551696778	0.0
5	0.0	0.0	0.0	0.734641849091453	0.0
6	0.0	0.0	0.0	1.4025138417380973	0.0
7	0.0	0.0	0.0	1.7839691644662965	0.0
8	0.0	0.0	0.0	2.766960193892726	0.0
9	0.0	0.0	0.0	3.4350923288372073	0.0
10	0.0	0.0	0.0	4.255667850314122	0.0
11	0.0	0.0	0.0	4.653512137806047	0.0
12	0.0	0.0	0.0	5.017537926579172	0.0
13	0.0	0.0	0.0	5.271957093863676	0.0
14	0.0	0.0	0.0	5.417983637049466	0.0
15	0.0	0.0	0.0	5.5236014099712545	0.0
16	0.0	0.0	0.0	5.66572581868949	0.0
17	0.0	0.0	0.0	5.800479528969013	0.0
18	0.0	0.0	0.0	6.044666432538599	0.0
19	0.0	0.0	0.0	6.152712200240198	0.0
20	0.0	0.0	0.0	6.281569351768751	0.0
21	0.0	0.0	0.0	6.404269802248496	0.0
22	0.0	0.0	0.0	6.534947949861908	0.0
23	0.0	0.0	0.0	6.690773186266221	0.0
24	0.0	0.0	0.0	6.816248487922894	0.0
25	0.0	0.0	0.0	6.93808179740985	0.0
26	0.0	0.0	0.0	7.050723412373235	0.0
27	0.0	0.0	0.0	7.195189101772002	0.0
28	0.0	0.0	0.0	7.318930121443096	0.0
29	0.0	0.0	0.0	7.449087984460833	0.0
30	0.0	0.0	0.0	7.629453310961096	0.0
31	0.0	0.0	0.0	7.7634265943471075	0.0
32	0.0	0.0	0.0	7.927836526580039	0.0
33	0.0	0.0	0.0	8.081580624601656	0.0
34	0.0	0.0	0.0	8.23159601635428	0.0
35	0.0	0.0	0.0	8.383865974688154	0.0
36	0.0	0.0	0.0	8.528331664086922	0.0
37	0.0	0.0	0.0	8.696296874390292	0.0
38	0.0	0.0	0.0	8.876055202195602	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	35	1.4460966E-7	44.0	1
CGTTTTT	9345	0.0	42.634563	1
ATAACGC	120	0.0	38.5	11
GCGTAAG	110	0.0	38.0	1
GTTTTTT	10820	0.0	37.981518	2
CGATTCG	35	7.2902276E-6	37.714287	10
ACGGGTA	100	0.0	37.399998	5
CGCATCG	120	0.0	36.666664	21
TAACGGG	265	0.0	36.5283	3
ACTAGGG	525	0.0	35.619045	3
ATAGGGC	705	0.0	35.57447	4
GACCGAT	695	0.0	35.453236	9
TATACTA	550	0.0	35.2	44
CATATGC	765	0.0	35.08497	33
AGGGATT	760	0.0	35.026318	6
GGGCGAT	2580	0.0	34.790695	7
CTACGGG	190	0.0	34.736843	3
AAGGGAC	945	0.0	34.68783	5
AGGGCGA	1390	0.0	34.66187	6
CGAATAT	70	2.7284841E-11	34.57143	14
>>END_MODULE