FastQCFastQC Report
Thu 26 May 2016
SRR1546487_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546487_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2434315
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188440.7740986684139071No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33870.13913565006993756No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG750.044.01
CGTTTTT118550.042.070011
TCGTTAG1500.041.0666661
TAGACGG3750.039.3066672
GCGTAAG1750.038.9714281
TGCGCTA357.293562E-637.71428740
AGGGATT27000.036.9111146
AGGGCGA27250.036.7339446
AGGGTAC10800.036.6666646
TACGGGA6900.036.6666644
TAACGCG301.301523E-436.6666641
CGTTAGG4100.036.48782
TTTAGCG1750.036.457141
ACGGGAT8500.036.2352945
GTTTTTT144200.035.88352
TAGTGCG1350.035.8518521
TAACGGG7650.035.660133
TATGGGA19050.035.5695534
TTGATCC15050.035.52159517
TAGGGAT27600.035.4710125
Produced by FastQC (version 0.11.5)