FastQCFastQC Report
Thu 26 May 2016
SRR1546486_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546486_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1530191
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130160.8506127666415499No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121930.7968286311970205No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCT15370.10044497713030595No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCGG254.4435343E-544.02
CGTTTTT159650.043.6279341
CGCCGGT1400.040.8571428
TAGTGCG650.040.6153831
GTTTTTA89950.040.404672
GAGCGAT7700.040.07
CGGTCTA3050.039.6721331
ATCGCGA501.3478711E-939.62
TGCGCGA750.038.133332
AGACCGA2150.037.8604668
CGACGGT3200.037.812528
CGTAAGA1400.037.7142832
TAATGCG1000.037.3999981
GCGTAAG1000.037.3999981
TCACGAC3300.037.33333225
GCGCGAC4500.037.1555569
CGGAGAT1600.037.1255
TATGCGA950.037.052632
ACGCCGG1550.036.90322527
GACCGAT3700.036.8648649
Produced by FastQC (version 0.11.5)