##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546486_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1530191
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.535566475034816	31.0	31.0	34.0	30.0	34.0
2	31.91180643462156	31.0	31.0	34.0	30.0	34.0
3	31.95435929240206	31.0	31.0	34.0	30.0	34.0
4	35.675612390871464	37.0	35.0	37.0	33.0	37.0
5	35.62912407666755	37.0	35.0	37.0	33.0	37.0
6	35.679764160160396	37.0	35.0	37.0	33.0	37.0
7	36.05230131401897	37.0	35.0	37.0	35.0	37.0
8	18.179175018020626	25.0	0.0	37.0	0.0	37.0
9	27.78314994664065	23.0	17.0	39.0	17.0	39.0
10	33.57526282666674	32.0	32.0	39.0	27.0	39.0
11	35.750812807028666	37.0	35.0	39.0	32.0	39.0
12	36.57354604751956	37.0	35.0	39.0	33.0	39.0
13	36.81458327751241	39.0	35.0	39.0	33.0	39.0
14	38.01487069261288	40.0	37.0	41.0	33.0	41.0
15	38.21774275237536	40.0	37.0	41.0	33.0	41.0
16	38.261138642169506	40.0	37.0	41.0	33.0	41.0
17	38.19839614793186	40.0	37.0	41.0	33.0	41.0
18	38.105963242497175	40.0	37.0	41.0	33.0	41.0
19	38.08862880516223	40.0	37.0	41.0	34.0	41.0
20	37.94064531813349	40.0	36.0	41.0	33.0	41.0
21	37.827775094743075	40.0	36.0	41.0	33.0	41.0
22	37.8136500606787	40.0	36.0	41.0	33.0	41.0
23	37.64966922429945	39.0	36.0	41.0	33.0	41.0
24	37.4466488170431	39.0	35.0	41.0	33.0	41.0
25	37.282886254068934	39.0	35.0	41.0	33.0	41.0
26	37.26848805149161	39.0	35.0	41.0	33.0	41.0
27	37.14500085283471	39.0	35.0	41.0	32.0	41.0
28	37.055176772050025	39.0	35.0	41.0	32.0	41.0
29	36.90044249378019	39.0	35.0	41.0	32.0	41.0
30	36.759194113676	39.0	35.0	40.0	31.0	41.0
31	36.63826934023269	39.0	35.0	41.0	31.0	41.0
32	36.302911205202484	39.0	35.0	41.0	30.0	41.0
33	36.02545760627268	39.0	35.0	41.0	28.0	41.0
34	35.75424701883621	39.0	35.0	41.0	25.0	41.0
35	35.489558493024724	39.0	35.0	41.0	24.0	41.0
36	35.34797551416784	39.0	35.0	41.0	23.0	41.0
37	35.287581092817824	39.0	35.0	41.0	23.0	41.0
38	35.198207282620274	39.0	35.0	41.0	23.0	41.0
39	35.10872172166743	39.0	35.0	41.0	23.0	41.0
40	35.04841879216385	39.0	35.0	40.0	22.0	41.0
41	35.01276311257875	39.0	35.0	40.0	22.0	41.0
42	34.834319375816484	38.0	35.0	40.0	21.0	41.0
43	34.8504631121213	39.0	35.0	40.0	21.0	41.0
44	34.61413509816748	38.0	34.0	40.0	20.0	41.0
45	34.54189771080865	38.0	34.0	40.0	20.0	41.0
46	34.53457770958005	38.0	34.0	40.0	20.0	41.0
47	34.44085869018966	38.0	34.0	40.0	20.0	41.0
48	34.41610818518734	38.0	34.0	40.0	20.0	41.0
49	34.369208811187626	38.0	34.0	40.0	20.0	41.0
50	33.775589452558535	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	2.0
13	3.0
14	8.0
15	19.0
16	81.0
17	196.0
18	525.0
19	1258.0
20	2385.0
21	4095.0
22	6571.0
23	9988.0
24	15453.0
25	23490.0
26	31497.0
27	35320.0
28	33749.0
29	32826.0
30	35925.0
31	43497.0
32	55007.0
33	71430.0
34	102605.0
35	150309.0
36	149844.0
37	229472.0
38	257489.0
39	237144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.432540774321637	22.461248301682602	27.03420684084536	20.072004083150404
2	36.86794655046331	23.702269847358927	27.198761461804445	12.231022140373327
3	26.403762667536274	23.16802281545245	37.48734635088038	12.940868166130896
4	23.41439728765886	25.519232566392034	37.10308059582104	13.963289550128055
5	21.055933540322744	29.64832494766993	35.70763388361322	13.588107628394102
6	18.821441244916485	38.063418226874944	32.62658060333645	10.488559924872124
7	81.3367742981105	2.8539574471422196	13.911792710844594	1.8974755439026894
8	41.30719629118195	50.67759514988652	6.744844271074657	1.2703642878568753
9	77.24212206188639	4.560672491211881	15.324949630470968	2.8722558164307594
10	36.8938910240617	32.74584676030639	20.43882103606674	9.921441179565166
11	27.612173905087666	23.087771395858425	35.11339434096789	14.186660358086018
12	26.87200486736623	20.737411212064377	35.48916442457183	16.90141949599756
13	21.975884056304082	25.950616622369367	35.35251481677778	16.720984504548777
14	16.613220179703056	29.729491285728383	35.88401709329097	17.77327144127759
15	16.51911428050485	24.525108303473225	43.06945995630611	15.886317459715812
16	19.407577224019747	24.042946272720204	40.088851653159644	16.460624850100412
17	19.003902127250782	23.33303489564375	35.43459607330065	22.22846690380482
18	20.589390474783865	24.253246816900635	37.088899359622424	18.068463348693072
19	22.944259899581162	26.621905369983224	32.748852920975224	17.68498180946039
20	24.240176553123106	25.349449839921945	32.458562362476314	17.95181124447863
21	22.191674111271077	27.130730738842406	33.69644704484603	16.981148105040482
22	20.018154596386985	23.963871176866157	34.85362284838951	21.164351378357342
23	18.281181891672347	26.48185749360701	34.649269274227855	20.58769134049279
24	19.24583270977283	24.074510959742934	37.91716197520441	18.76249435527983
25	18.877774081797632	26.23535231876282	35.280693717320254	19.60617988211929
26	17.63479199655468	28.539835876697744	33.88309041158914	19.942281715158437
27	18.0064449470687	26.497476458821158	35.307814514658624	20.18826407945152
28	17.727394815418467	25.841610622464778	37.86292038052766	18.568074181589093
29	18.419138525844158	24.225276452416725	37.00198210550186	20.353602916237254
30	19.829812095352803	25.191757107446065	35.495568853822824	19.48286194337831
31	22.648414478976807	24.374146756842773	33.405306919201585	19.57213184497883
32	23.006801111756637	24.847028900313752	32.87720291127055	19.268967076659056
33	21.962487035932117	25.421205588060573	32.80976035017851	19.806547025828802
34	18.875160029042128	25.43440655447588	34.546798406212034	21.14363501026996
35	18.33490067579799	26.81632554367396	33.686317590418454	21.1624561901096
36	21.26812927275092	27.127397821579137	31.984830651859802	19.619642253810145
37	20.239695567416092	29.3073871170331	31.036386960843448	19.416530354707355
38	19.778968769258217	28.829538273326666	30.512988247872325	20.878504709542796
39	20.90941588337665	28.337442842102718	29.870846188482354	20.88229508603828
40	22.265194345019673	25.503090790626793	30.413131432612005	21.81858343174153
41	19.326018778047967	25.682284107016706	31.009200812186187	23.982496302749134
42	20.77805973241249	27.035579218542	29.215307108720413	22.9710539403251
43	20.59174312226382	26.171700134166258	30.499721930138136	22.736834813431788
44	20.183428081853837	27.414159408858108	30.764329420314198	21.63808308897386
45	19.458878009346545	28.344435433223698	29.75759235285007	22.43909420457969
46	20.78139264967576	28.790654238588516	29.677406284574932	20.750546827160793
47	19.969467863815694	26.507148454016527	30.439860122037054	23.08352356013073
48	20.177481111835057	24.4956348586549	32.429023566339104	22.89786046317094
49	21.325377028096494	23.781475645850747	32.35687571028715	22.536271615765614
50	19.257203839259283	26.143337661769024	32.33112729064542	22.26833120832628
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	418.0
1	985.0
2	1552.0
3	21252.5
4	40953.0
5	28622.5
6	16292.0
7	15993.5
8	15695.0
9	15845.5
10	15996.0
11	15978.5
12	15961.0
13	15309.5
14	14658.0
15	13933.0
16	13208.0
17	12648.5
18	12089.0
19	11426.0
20	10763.0
21	10769.0
22	10775.0
23	11716.5
24	12658.0
25	14595.5
26	16533.0
27	18873.0
28	21213.0
29	25025.5
30	28838.0
31	33373.0
32	37908.0
33	42668.5
34	47429.0
35	52589.5
36	57750.0
37	63471.0
38	69192.0
39	77383.0
40	85574.0
41	94965.0
42	104356.0
43	110962.5
44	117569.0
45	123415.5
46	129262.0
47	131675.0
48	134088.0
49	125741.0
50	117394.0
51	103465.0
52	89536.0
53	80069.0
54	70602.0
55	65155.5
56	59709.0
57	55898.5
58	52088.0
59	47250.0
60	42412.0
61	36194.0
62	29976.0
63	23599.0
64	17222.0
65	13039.0
66	8856.0
67	6834.5
68	4813.0
69	3962.0
70	3111.0
71	2579.0
72	2047.0
73	1513.5
74	980.0
75	692.5
76	405.0
77	273.5
78	142.0
79	119.5
80	97.0
81	62.5
82	28.0
83	24.5
84	21.0
85	17.5
86	14.0
87	9.5
88	5.0
89	2.5
90	0.0
91	1.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1530191.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.15944486809867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.57606433825518	30.176071383441517
2	8.628643059549479	7.275576027111569
3	4.62287637282268	5.846937047161611
4	3.112184275731523	5.248318455682669
5	2.340024421100412	4.932706528569366
6	1.8379787304871067	4.649289777402549
7	1.511637499153911	4.461085846429089
8	1.1729165450372623	3.955960833230338
9	0.9582697986663268	3.6360110471073313
>10	4.193074108791916	25.49378867133688
>50	0.030232054960615873	0.8405916624544707
>100	0.013684021965644033	1.0801703858286034
>500	0.001770833883869112	0.507059306727389
>1k	3.2196979706711134E-4	0.18548963797161744
>5k	0.0	0.0
>10k+	3.2196979706711134E-4	1.7109433895450072
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13016	0.8506127666415499	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12193	0.7968286311970205	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCT	1537	0.10044497713030595	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04339327574139437	0.0
2	0.0	0.0	0.0	0.1390022552740148	0.0
3	0.0	0.0	0.0	0.2408849614198489	0.0
4	0.0	0.0	0.0	0.37374419271842535	0.0
5	0.0	0.0	0.0	0.6678904790317026	0.0
6	0.0	0.0	0.0	1.1963865948760646	0.0
7	0.0	0.0	0.0	1.5707843007833662	0.0
8	0.0	0.0	0.0	2.23808661794508	0.0
9	0.0	0.0	0.0	2.986620624484133	0.0
10	0.0	0.0	0.0	3.854028680079807	0.0
11	0.0	0.0	0.0	4.352201783960303	0.0
12	0.0	0.0	0.0	4.806720206823854	0.0
13	0.0	0.0	0.0	5.087796229359602	0.0
14	0.0	0.0	0.0	5.242482801166652	0.0
15	0.0	0.0	0.0	5.359134905381093	0.0
16	0.0	0.0	0.0	5.552966917201839	0.0
17	0.0	0.0	0.0	5.765162649630014	0.0
18	0.0	0.0	0.0	6.116033880737764	0.0
19	0.0	0.0	0.0	6.276994179158026	0.0
20	0.0	0.0	0.0	6.489974127412852	0.0
21	0.0	0.0	0.0	6.676813548112621	0.0
22	0.0	0.0	0.0	6.8852842553641995	0.0
23	0.0	0.0	0.0	7.119895490170835	0.0
24	0.0	0.0	0.0	7.30183356195403	0.0
25	0.0	0.0	0.0	7.458088565414383	0.0
26	0.0	0.0	0.0	7.6114027595247915	0.0
27	0.0	0.0	0.0	7.796739099890145	0.0
28	0.0	0.0	0.0	7.966652528998014	0.0
29	0.0	0.0	0.0	8.156367407728839	0.0
30	0.0	0.0	0.0	8.400454583774183	0.0
31	0.0	0.0	0.0	8.59676994571266	0.0
32	0.0	0.0	0.0	8.801123519874317	0.0
33	0.0	0.0	0.0	9.011162658779199	0.0
34	0.0	0.0	0.0	9.253289295257913	0.0
35	0.0	0.0	0.0	9.485809287860143	0.0
36	0.0	0.0	0.0	9.703298477118215	0.0
37	0.0	0.0	0.0	9.926603933757288	0.0
38	0.0	0.0	0.0	10.186375426335667	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACCGG	25	4.4435343E-5	44.0	2
CGTTTTT	15965	0.0	43.627934	1
CGCCGGT	140	0.0	40.85714	28
TAGTGCG	65	0.0	40.615383	1
GTTTTTA	8995	0.0	40.40467	2
GAGCGAT	770	0.0	40.0	7
CGGTCTA	305	0.0	39.67213	31
ATCGCGA	50	1.3478711E-9	39.6	2
TGCGCGA	75	0.0	38.13333	2
AGACCGA	215	0.0	37.860466	8
CGACGGT	320	0.0	37.8125	28
CGTAAGA	140	0.0	37.714283	2
TAATGCG	100	0.0	37.399998	1
GCGTAAG	100	0.0	37.399998	1
TCACGAC	330	0.0	37.333332	25
GCGCGAC	450	0.0	37.155556	9
CGGAGAT	160	0.0	37.125	5
TATGCGA	95	0.0	37.05263	2
ACGCCGG	155	0.0	36.903225	27
GACCGAT	370	0.0	36.864864	9
>>END_MODULE