FastQCFastQC Report
Thu 26 May 2016
SRR1546485_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546485_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1337933
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97020.7251484192407243No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91230.6818727096199884No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA1350.044.0000042
TGCGCGA551.8189894E-1244.0000042
CTTGCGA408.314601E-944.0000042
ACGTGCG408.314601E-944.0000041
TCGCGGA207.856944E-444.0000042
AACGCGA254.4432323E-544.02
GCGATCG351.4463149E-744.09
TCGCGCG302.5280297E-644.01
TAATCGT454.802132E-1044.021
CGTTTTT117800.043.365031
GTTTGCG1100.042.0000041
GAGCGAT7600.041.3947337
TATTGCG800.041.2500041
TTTGCGA2100.040.857142
GGTACGA557.8216544E-1140.0000042
CGACGGT1650.040.028
ACGAGAC2150.039.9069755
GTTTTTA65800.039.820672
CGGGTAC452.3514076E-839.111116
GCAACGG452.3514076E-839.111112
Produced by FastQC (version 0.11.5)