##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546485_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1337933
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59671373678652	31.0	31.0	34.0	30.0	34.0
2	31.97270341638931	31.0	31.0	34.0	30.0	34.0
3	32.02565823550208	33.0	31.0	34.0	30.0	34.0
4	35.72470445082078	37.0	35.0	37.0	35.0	37.0
5	35.674585349191624	37.0	35.0	37.0	33.0	37.0
6	35.7266185974933	37.0	35.0	37.0	33.0	37.0
7	36.10614956055348	37.0	35.0	37.0	35.0	37.0
8	18.347311860907833	28.0	0.0	37.0	0.0	37.0
9	27.915319376979266	27.0	17.0	39.0	17.0	39.0
10	33.67855116810782	32.0	32.0	39.0	27.0	39.0
11	35.80157825541339	37.0	35.0	39.0	32.0	39.0
12	36.56991269368496	37.0	35.0	39.0	33.0	39.0
13	36.76367052759742	39.0	35.0	39.0	33.0	39.0
14	37.94304124347034	40.0	37.0	41.0	33.0	41.0
15	38.18557730469313	40.0	37.0	41.0	33.0	41.0
16	38.26921079007693	40.0	37.0	41.0	34.0	41.0
17	38.240763177229354	40.0	37.0	41.0	34.0	41.0
18	38.164690608573075	40.0	37.0	41.0	33.0	41.0
19	38.14762398416064	40.0	37.0	41.0	34.0	41.0
20	38.012707661743896	40.0	36.0	41.0	34.0	41.0
21	37.88959237869161	40.0	36.0	41.0	33.0	41.0
22	37.890495263963146	40.0	36.0	41.0	33.0	41.0
23	37.739168553283314	39.0	36.0	41.0	33.0	41.0
24	37.55956090476877	39.0	35.0	41.0	33.0	41.0
25	37.43479382001939	39.0	35.0	41.0	33.0	41.0
26	37.38632203555783	39.0	35.0	41.0	33.0	41.0
27	37.2391943393279	39.0	35.0	41.0	33.0	41.0
28	37.22677742457956	39.0	35.0	41.0	33.0	41.0
29	37.14155641575475	39.0	35.0	41.0	32.0	41.0
30	37.016049383638794	39.0	35.0	41.0	32.0	41.0
31	36.91207631473325	39.0	35.0	41.0	31.0	41.0
32	36.60124161673268	39.0	35.0	41.0	31.0	41.0
33	36.3696799466042	39.0	35.0	41.0	30.0	41.0
34	36.17675324549137	39.0	35.0	41.0	30.0	41.0
35	35.95093401538044	39.0	35.0	41.0	27.0	41.0
36	35.8141027988696	39.0	35.0	41.0	27.0	41.0
37	35.7576635003397	39.0	35.0	41.0	26.0	41.0
38	35.69992144599169	39.0	35.0	41.0	26.0	41.0
39	35.62977294079749	39.0	35.0	41.0	25.0	41.0
40	35.56491991751455	39.0	35.0	41.0	25.0	41.0
41	35.56429058854218	39.0	35.0	41.0	25.0	41.0
42	35.40344471658895	39.0	35.0	41.0	24.0	41.0
43	35.42047695960859	39.0	35.0	41.0	24.0	41.0
44	35.18354805509693	39.0	35.0	40.0	23.0	41.0
45	35.100571553284055	38.0	35.0	40.0	23.0	41.0
46	35.10827896464173	38.0	35.0	40.0	23.0	41.0
47	34.992864366152865	38.0	34.0	40.0	23.0	41.0
48	34.98494917159529	38.0	34.0	40.0	23.0	41.0
49	34.9657322152903	38.0	34.0	40.0	23.0	41.0
50	34.390038215665506	38.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	4.0
14	5.0
15	15.0
16	64.0
17	145.0
18	337.0
19	814.0
20	1600.0
21	2868.0
22	4625.0
23	7221.0
24	11406.0
25	17405.0
26	23388.0
27	25786.0
28	25357.0
29	25523.0
30	28924.0
31	36282.0
32	46534.0
33	61515.0
34	91378.0
35	141359.0
36	131051.0
37	204113.0
38	235383.0
39	214827.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.39123259535418	23.484957766943488	28.83290867330427	18.29090096439807
2	35.19227046496349	24.089920795734916	28.3714505883329	12.346358150968696
3	26.32261854666863	24.09455480954577	36.687935793496386	12.894890850289215
4	23.744163571718463	26.60394803028253	35.99858886805244	13.653299529946567
5	21.53448640552255	30.45077743055893	34.624379546658915	13.390356617259608
6	19.15357495479968	38.72839671343782	31.418688379761917	10.699339952000585
7	83.4819082868873	2.956874522117326	11.84558569076329	1.7156315002320743
8	42.87785711242641	50.25371225614437	5.696772558865056	1.1716580725641719
9	79.90258107095049	4.397679106502343	12.9169397869699	2.7828000355772673
10	39.41684673298289	32.971531459348114	18.042308546093118	9.569313261575878
11	30.224682401884102	22.71152591347997	32.95733044928259	14.10646123535334
12	28.631030103899075	21.88390599529274	32.46470488432529	17.02035901648289
13	20.598938810837314	29.73183261045209	33.79010757638836	15.879121002322238
14	16.157983994714233	33.03648239485833	34.33557584722105	16.469957763206377
15	15.230209584485921	25.69657822925363	43.67610336242547	15.397108823834976
16	17.6668039430973	23.644308048310343	41.671294451964336	17.01759355662802
17	18.14403262345723	23.27560498171433	33.80557920314396	24.774783191684485
18	19.82916932312754	24.57507214486824	36.70684555953101	18.88891297247321
19	23.226873094542103	26.049361216144607	31.838216113960865	18.885549575352428
20	24.830690326047716	25.789482731945473	31.262253042566407	18.117573899440405
21	21.247850228673634	26.990215504064853	33.288139241651116	18.4737950256104
22	20.362006169217743	23.792222779466535	33.34090720536828	22.504863845947444
23	18.03102248019893	27.404586029345268	33.35436079385141	21.21003069660439
24	18.191344409622904	25.14826975640783	38.57876291264211	18.08162292132715
25	17.611943198949422	27.020336593835413	36.30069667165695	19.06702353555821
26	17.72278581961877	31.54395623697151	32.19951970689115	18.53373823651857
27	18.41437500980991	28.79449120396911	34.04677214778318	18.7443616384378
28	16.932462238393104	26.84783169261839	38.52965731467869	17.69004875430982
29	17.420752758172494	24.297928222115754	37.95436692270839	20.32695209700336
30	19.70733960519697	26.84020799247795	34.98508520232328	18.467367200001796
31	23.58877462473831	25.318308166402954	31.70218538596477	19.390731822893972
32	24.224979875673895	25.824462062001608	31.707566821358018	18.242991240966475
33	22.817734520338462	25.92282274224494	31.83320838935881	19.42623434805779
34	19.222038771747165	25.94778662309697	33.201961533200844	21.628213071955024
35	19.624076840918043	25.122782680448125	33.60579341416947	21.647347064464366
36	23.445867618184167	25.162844477264557	32.00406896309456	19.38721894145671
37	20.48742351074381	28.132126197649658	32.48256826014457	18.89788203146196
38	19.391329760159888	28.821697349568325	30.509748993409985	21.277223896861802
39	20.19024868958311	28.24864922234521	31.160080512252858	20.40102157581882
40	21.96933628216062	25.933510870873206	30.2480766974131	21.849076149553078
41	18.566774270460478	25.574150574057146	31.038848731588203	24.82022642389417
42	21.04500001121132	26.46806678660292	29.14368656726458	23.34324663492118
43	20.883482207255522	26.329719051701396	30.680310598512783	22.106488142530306
44	20.203179082958563	28.79374378238671	30.676648232759042	20.326428901895685
45	19.166281121700415	30.22849425195432	28.92177709945117	21.6834475268941
46	21.44479581563501	28.73611757838397	29.430173259797016	20.388913346184
47	20.45356531306127	26.443626100858562	30.82957068851729	22.273237897562883
48	20.8987296075364	24.840481548777106	32.43204256117459	21.828746282511904
49	20.203104340800323	24.319902416638204	32.92040782311222	22.556585419449256
50	19.10573997352633	28.174953454320956	31.48550786922813	21.233798702924584
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	404.0
1	800.0
2	1196.0
3	15008.5
4	28821.0
5	20045.5
6	11270.0
7	11255.0
8	11240.0
9	11541.5
10	11843.0
11	11803.0
12	11763.0
13	11454.0
14	11145.0
15	10612.5
16	10080.0
17	9465.5
18	8851.0
19	8724.0
20	8597.0
21	8987.0
22	9377.0
23	10148.0
24	10919.0
25	12753.0
26	14587.0
27	17123.0
28	19659.0
29	23347.5
30	27036.0
31	30443.5
32	33851.0
33	37783.0
34	41715.0
35	46610.5
36	51506.0
37	57819.5
38	64133.0
39	73379.5
40	82626.0
41	93687.0
42	104748.0
43	112366.5
44	119985.0
45	122884.5
46	125784.0
47	124492.5
48	123201.0
49	113894.0
50	104587.0
51	92122.5
52	79658.0
53	69992.0
54	60326.0
55	52718.0
56	45110.0
57	40032.5
58	34955.0
59	30750.0
60	26545.0
61	22268.0
62	17991.0
63	14479.5
64	10968.0
65	8490.5
66	6013.0
67	4659.5
68	3306.0
69	2673.5
70	2041.0
71	1578.5
72	1116.0
73	815.0
74	514.0
75	374.0
76	234.0
77	177.5
78	121.0
79	94.0
80	67.0
81	47.0
82	27.0
83	15.5
84	4.0
85	5.5
86	7.0
87	5.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1337933.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.255153240756705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.38243351659862	26.24236219083167
2	7.474942694611135	5.420103857180031
3	3.6950263506959082	4.018912397193426
4	2.5433880657383523	3.6884369629622293
5	1.9468361428560435	3.529142134694478
6	1.6153131487811299	3.5138055444521457
7	1.3162801992075808	3.3405358231031217
8	1.1669063513501188	3.384509486864867
9	1.0158936267410839	3.314824120242548
>10	6.783024996761089	39.607973824237945
>50	0.04627516866048228	1.0795045579384945
>100	0.011192515427383394	0.8332893138798705
>500	0.0016581483807378457	0.4326128136162066
>1k	4.145370951844614E-4	0.17937237903454667
>5k	4.145370951844614E-4	1.4146145937684713
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9702	0.7251484192407243	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9123	0.6818727096199884	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03624994674621226	0.0
2	0.0	0.0	0.0	0.1449250448266094	0.0
3	0.0	0.0	0.0	0.2630176548451978	0.0
4	0.0	0.0	0.0	0.41018496441899555	0.0
5	0.0	0.0	0.0	0.7582591953408728	0.0
6	0.0	0.0	0.0	1.2715135959722945	0.0
7	0.0	0.0	0.0	1.606806917835198	0.0
8	0.0	0.0	0.0	2.223878176261442	0.0
9	0.0	0.0	0.0	2.803204644776682	0.0
10	0.0	0.0	0.0	3.468335110950997	0.0
11	0.0	0.0	0.0	3.9924271245271625	0.0
12	0.0	0.0	0.0	4.420475464765426	0.0
13	0.0	0.0	0.0	4.65127924940935	0.0
14	0.0	0.0	0.0	4.768773922162022	0.0
15	0.0	0.0	0.0	4.88499797822462	0.0
16	0.0	0.0	0.0	5.0894925231682	0.0
17	0.0	0.0	0.0	5.342345244492811	0.0
18	0.0	0.0	0.0	5.6783859879381104	0.0
19	0.0	0.0	0.0	5.866362515910737	0.0
20	0.0	0.0	0.0	6.0662230470434615	0.0
21	0.0	0.0	0.0	6.2829005637801	0.0
22	0.0	0.0	0.0	6.5300728810785	0.0
23	0.0	0.0	0.0	6.806394640090348	0.0
24	0.0	0.0	0.0	7.013729387047034	0.0
25	0.0	0.0	0.0	7.1878786157453325	0.0
26	0.0	0.0	0.0	7.357169604158056	0.0
27	0.0	0.0	0.0	7.5435765468076506	0.0
28	0.0	0.0	0.0	7.742091719092062	0.0
29	0.0	0.0	0.0	7.952191925903614	0.0
30	7.47421582396129E-5	0.0	0.0	8.21573277585649	0.0
31	7.47421582396129E-5	0.0	0.0	8.438688633885254	0.0
32	7.47421582396129E-5	0.0	0.0	8.645126474943066	0.0
33	7.47421582396129E-5	0.0	0.0	8.850218957152563	0.0
34	7.47421582396129E-5	0.0	0.0	9.067569153313357	0.0
35	7.47421582396129E-5	0.0	0.0	9.32206620211924	0.0
36	7.47421582396129E-5	0.0	0.0	9.541060725761305	0.0
37	7.47421582396129E-5	0.0	0.0	9.780459858602786	0.0
38	7.47421582396129E-5	0.0	0.0	10.014552298209253	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGA	135	0.0	44.000004	2
TGCGCGA	55	1.8189894E-12	44.000004	2
CTTGCGA	40	8.314601E-9	44.000004	2
ACGTGCG	40	8.314601E-9	44.000004	1
TCGCGGA	20	7.856944E-4	44.000004	2
AACGCGA	25	4.4432323E-5	44.0	2
GCGATCG	35	1.4463149E-7	44.0	9
TCGCGCG	30	2.5280297E-6	44.0	1
TAATCGT	45	4.802132E-10	44.0	21
CGTTTTT	11780	0.0	43.36503	1
GTTTGCG	110	0.0	42.000004	1
GAGCGAT	760	0.0	41.394733	7
TATTGCG	80	0.0	41.250004	1
TTTGCGA	210	0.0	40.85714	2
GGTACGA	55	7.8216544E-11	40.000004	2
CGACGGT	165	0.0	40.0	28
ACGAGAC	215	0.0	39.906975	5
GTTTTTA	6580	0.0	39.82067	2
CGGGTAC	45	2.3514076E-8	39.11111	6
GCAACGG	45	2.3514076E-8	39.11111	2
>>END_MODULE