FastQCFastQC Report
Thu 26 May 2016
SRR1546484_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546484_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1101086
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45120.40977725627244377No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41960.38107831722499425No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG207.856336E-444.0000041
ATCCCGT207.856336E-444.00000421
TATGCGA502.7284841E-1144.02
TACGATG351.4460056E-744.01
GTAACGA454.802132E-1044.02
CGTTTTT52850.042.584671
CGTAAGA1050.041.9047622
TACCGGT603.6379788E-1240.33333240
TTTAGCG501.3478711E-939.6000021
ACCGGTA501.3478711E-939.60000241
GCGTAAG950.039.3684231
GTTTTTA30400.039.1513182
TGTACGA900.039.111112
AGACTTA7000.038.0285728
CGACGGT2800.037.71428728
CTAACGA357.2899293E-637.7142872
TACGTAG357.2899293E-637.7142871
ACGGTCT2850.037.0526330
TTAGCGA1200.036.6666642
TACGAGT301.3010597E-436.6666644
Produced by FastQC (version 0.11.5)