##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546484_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1101086
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53258146956732	31.0	31.0	33.0	30.0	34.0
2	31.910081501354117	31.0	31.0	34.0	30.0	34.0
3	32.00837445939736	33.0	31.0	34.0	30.0	34.0
4	35.69880463469702	37.0	35.0	37.0	35.0	37.0
5	35.65708582254247	37.0	35.0	37.0	33.0	37.0
6	35.698764674148975	37.0	35.0	37.0	33.0	37.0
7	36.03392287250951	37.0	35.0	37.0	35.0	37.0
8	18.307577246463946	28.0	0.0	37.0	0.0	37.0
9	27.848099058565815	27.0	17.0	39.0	17.0	39.0
10	33.634311942936336	32.0	32.0	39.0	27.0	39.0
11	35.830623584352175	37.0	35.0	39.0	32.0	39.0
12	36.44462739513535	37.0	35.0	39.0	32.0	39.0
13	36.57497507006719	39.0	35.0	39.0	33.0	39.0
14	37.702910581008204	39.0	36.0	41.0	33.0	41.0
15	37.95259589169238	40.0	36.0	41.0	33.0	41.0
16	38.02824393371635	40.0	36.0	41.0	33.0	41.0
17	37.936076746048904	40.0	36.0	41.0	33.0	41.0
18	37.89123737837008	40.0	36.0	41.0	33.0	41.0
19	37.91060643764429	40.0	36.0	41.0	33.0	41.0
20	37.82317729950249	40.0	36.0	41.0	33.0	41.0
21	37.68827230570546	40.0	35.0	41.0	33.0	41.0
22	37.680307441925514	40.0	35.0	41.0	33.0	41.0
23	37.57508314518575	39.0	35.0	41.0	33.0	41.0
24	37.44777519648783	39.0	35.0	41.0	33.0	41.0
25	37.310371760243974	39.0	35.0	41.0	32.0	41.0
26	37.27154191407392	39.0	35.0	41.0	33.0	41.0
27	37.14979483891358	39.0	35.0	41.0	32.0	41.0
28	37.12355892273628	39.0	35.0	41.0	32.0	41.0
29	37.00518942207965	39.0	35.0	41.0	32.0	41.0
30	36.8809711503007	39.0	35.0	40.0	31.0	41.0
31	36.88018556225399	39.0	35.0	41.0	31.0	41.0
32	36.68148173712135	39.0	35.0	41.0	31.0	41.0
33	36.571097988712964	39.0	35.0	41.0	30.0	41.0
34	36.41415021170008	39.0	35.0	41.0	30.0	41.0
35	36.236640916331694	39.0	35.0	41.0	30.0	41.0
36	36.116457751710584	39.0	35.0	41.0	30.0	41.0
37	36.06302323342591	39.0	35.0	41.0	29.0	41.0
38	35.97015582797347	39.0	35.0	41.0	29.0	41.0
39	35.918748399307596	39.0	35.0	40.0	29.0	41.0
40	35.834996539779816	38.0	35.0	40.0	29.0	41.0
41	35.81597441071815	38.0	35.0	40.0	29.0	41.0
42	35.67698254268967	38.0	35.0	40.0	28.0	41.0
43	35.683132834310854	38.0	35.0	40.0	28.0	41.0
44	35.431804600185636	38.0	35.0	40.0	27.0	41.0
45	35.33214390156627	38.0	34.0	40.0	27.0	41.0
46	35.32512991719084	38.0	34.0	40.0	27.0	41.0
47	35.20222398613732	38.0	34.0	40.0	26.0	41.0
48	35.19283779831911	38.0	34.0	40.0	27.0	41.0
49	35.14551906027322	37.0	34.0	40.0	27.0	41.0
50	34.53888797060357	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	1.0
13	7.0
14	12.0
15	26.0
16	55.0
17	137.0
18	298.0
19	648.0
20	1220.0
21	2133.0
22	3343.0
23	5059.0
24	7563.0
25	11035.0
26	14420.0
27	17077.0
28	18844.0
29	21005.0
30	25698.0
31	32122.0
32	41603.0
33	55429.0
34	86140.0
35	127455.0
36	116207.0
37	175677.0
38	186386.0
39	151480.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.71708840181421	23.129710122551735	27.29932085232216	14.853880623311893
2	34.35717101116534	23.72848260717147	28.674054524351412	13.240291857311782
3	30.33913790566768	24.733762848678488	31.599166640934495	13.327932604719342
4	26.521634095792702	26.400299340832596	31.707695856636086	15.37037070673862
5	22.96369220932788	30.648468875274048	30.66899406585862	15.718844849539455
6	20.697111760570927	40.00686594870882	28.450729552460025	10.845292738260227
7	85.71728275538877	3.67128453181677	8.466277838424974	2.145154874369486
8	43.74853553673373	50.73245868170152	4.11684464247116	1.4021611390935858
9	81.51488621233945	4.922776240911246	10.080956437553471	3.48138110919583
10	43.624657837807405	29.209162590388033	15.173474188210548	11.992705383594016
11	39.54940849306957	21.784401944988858	23.631669097600007	15.034520464341568
12	35.64780589345428	20.48277791198871	26.69555329919734	17.173862895359672
13	24.991417564113974	28.18807976851944	28.26827332288304	18.552229344483536
14	18.848391497121934	31.248876109586355	31.14561442067195	18.757117972619756
15	18.68900340209575	23.117903596994243	39.99142664605671	18.201666354853298
16	22.764797663397772	20.388234888101383	37.83155902445404	19.015408424046804
17	21.603943742813914	21.159110187578445	30.520504302116276	26.716441767491368
18	24.404088327342276	23.19310208285275	31.701701774429974	20.701107815375003
19	27.39631600074835	26.619719077347277	26.495478100711477	19.488486821192897
20	29.61812247181419	25.492105067179132	25.57084551070489	19.318926950301794
21	25.62097783461056	27.32238898687296	28.047128017248426	19.009505161268056
22	23.595159687799136	22.99747703630779	29.432850839988884	23.974512435904188
23	22.583976183513368	26.760761648045655	28.480790782917953	22.17447138552302
24	23.929102722221515	24.170591579585974	32.16324610430066	19.73705959389185
25	22.711305020679585	24.07813740252805	30.067496998417926	23.143060578374442
26	19.590567857551545	28.761422813476877	28.869952029178464	22.778057299793115
27	20.668685279805572	26.11993976855577	30.361116207090088	22.85025874454856
28	19.89272409239605	26.147548874474836	33.323464288892964	20.636262744236145
29	21.373171577878566	25.16270300412502	32.522073661821146	20.942051756175267
30	23.2147171065657	24.79170564333758	30.772619032482478	21.220958217614246
31	28.31050435660793	23.52032447965009	26.489393199078	21.679777964663977
32	28.898741787653282	24.868448059461297	25.757751892222768	20.475058260662653
33	27.953584007062123	24.592357000270642	26.393033786643365	21.06102520602387
34	21.82163791020865	26.272425587102187	29.52076404567854	22.385172457010626
35	22.401519953936386	26.314747440254436	29.360740214660797	21.922992391148373
36	26.608003371217144	27.468426626076436	25.495283747136916	20.428286255569503
37	24.304731873804588	29.285269270520196	25.84148740425362	20.568511451421596
38	23.607420310493456	30.604330633574488	24.09412162174435	21.694127434187703
39	23.587621675327814	27.82244075394656	25.379943074382926	23.209994496342702
40	23.907578517935928	24.347780282375766	27.13729899390238	24.607342205785923
41	20.236566444401255	25.252795876071442	27.37115901936815	27.13947866015915
42	22.318420177897096	27.39577108418416	24.632135909456665	25.65367282846208
43	21.599039493736182	25.843939528792482	26.35534372428675	26.201677253184585
44	22.280548476685745	26.32764379894032	27.997722248761676	23.394085475612258
45	21.644721665700953	28.848155366610783	25.696539598178525	23.81058336950974
46	23.421694581531323	27.660509714954145	27.236292169730614	21.681503533783918
47	22.142230488808323	26.112492575511816	27.665413964031877	24.07986297164799
48	22.716754186321506	23.976601282733593	29.620120499216227	23.686524031728677
49	23.10128364178638	23.366476369693192	30.023358756718366	23.508881231802057
50	20.918257066205545	25.797076704271966	29.264380802226164	24.020285427296322
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	333.0
1	869.0
2	1405.0
3	8114.5
4	14824.0
5	10188.0
6	5552.0
7	5506.0
8	5460.0
9	5568.5
10	5677.0
11	5584.5
12	5492.0
13	5350.0
14	5208.0
15	4997.5
16	4787.0
17	4591.0
18	4395.0
19	4518.5
20	4642.0
21	4919.5
22	5197.0
23	5593.0
24	5989.0
25	6945.5
26	7902.0
27	9134.0
28	10366.0
29	12127.5
30	13889.0
31	16213.5
32	18538.0
33	21612.0
34	24686.0
35	27345.5
36	30005.0
37	33149.0
38	36293.0
39	43853.5
40	51414.0
41	60592.5
42	69771.0
43	74957.5
44	80144.0
45	82935.0
46	85726.0
47	86452.5
48	87179.0
49	85259.0
50	83339.0
51	77858.0
52	72377.0
53	67833.5
54	63290.0
55	60621.0
56	57952.0
57	56794.0
58	55636.0
59	52891.5
60	50147.0
61	46776.0
62	43405.0
63	37144.0
64	30883.0
65	25895.5
66	20908.0
67	17382.5
68	13857.0
69	11738.5
70	9620.0
71	8147.0
72	6674.0
73	5317.0
74	3960.0
75	3060.0
76	2160.0
77	1628.0
78	1096.0
79	809.0
80	522.0
81	387.0
82	252.0
83	167.0
84	82.0
85	55.0
86	28.0
87	22.5
88	17.0
89	11.0
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1101086.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.63811288145241
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.51375115789145	23.046732953808934
2	7.295421881705873	4.908084495492802
3	4.03500053278361	4.071894101954872
4	2.988704247731398	4.021374834178602
5	2.366545364262033	3.9803060051062085
6	2.094436877701322	4.227174246911628
7	1.7611047756284346	4.146816886705794
8	1.566541015190525	4.215638680192315
9	1.3911349648543312	4.21156394829948
>10	7.925613282336546	40.049526095606154
>50	0.04008277919221472	0.8802974679197815
>100	0.020309175702659294	1.2316961533273516
>500	5.415780078259147E-4	0.1226048663459544
>1k	8.123670117388721E-4	0.8862892641501755
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4512	0.40977725627244377	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4196	0.38107831722499425	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022977315123432682	0.0
2	0.0	0.0	0.0	0.07247390303754657	0.0
3	0.0	0.0	0.0	0.1236052406442367	0.0
4	0.0	0.0	0.0	0.20107421218687732	0.0
5	0.0	0.0	0.0	0.3785353732587645	0.0
6	0.0	0.0	0.0	0.6543539741673221	0.0
7	0.0	0.0	0.0	0.8308161215381905	0.0
8	0.0	0.0	0.0	1.2602103741215491	0.0
9	0.0	0.0	0.0	1.7197566765902028	0.0
10	0.0	0.0	0.0	2.294734471240212	0.0
11	0.0	0.0	0.0	2.683714078645991	0.0
12	0.0	0.0	0.0	2.984508022080019	0.0
13	0.0	0.0	0.0	3.1287292727361895	0.0
14	0.0	0.0	0.0	3.1961172878412767	0.0
15	0.0	0.0	0.0	3.271497412554514	0.0
16	0.0	0.0	0.0	3.410087858714033	0.0
17	0.0	0.0	0.0	3.5619379412688925	0.0
18	0.0	0.0	0.0	3.789713065101182	0.0
19	0.0	0.0	0.0	3.91504387486536	0.0
20	0.0	0.0	0.0	4.0456422114167285	0.0
21	0.0	0.0	0.0	4.198218849390511	0.0
22	0.0	0.0	0.0	4.368777733982632	0.0
23	0.0	0.0	0.0	4.567944738194837	0.0
24	0.0	0.0	0.0	4.721520389869638	0.0
25	0.0	0.0	0.0	4.849575782454776	0.0
26	0.0	0.0	0.0	4.979084285877761	0.0
27	0.0	0.0	0.0	5.105323289915592	0.0
28	0.0	0.0	0.0	5.245094388630861	0.0
29	9.081942736534657E-5	0.0	0.0	5.392312680390088	0.0
30	9.081942736534657E-5	0.0	0.0	5.573043340847128	0.0
31	9.081942736534657E-5	0.0	0.0	5.733702907856426	0.0
32	9.081942736534657E-5	0.0	0.0	5.890820517198475	0.0
33	9.081942736534657E-5	0.0	0.0	6.054931222447657	0.0
34	9.081942736534657E-5	0.0	0.0	6.224127815629297	0.0
35	9.081942736534657E-5	0.0	0.0	6.417754834772216	0.0
36	9.081942736534657E-5	0.0	0.0	6.595306815271468	0.0
37	9.081942736534657E-5	0.0	0.0	6.78693580701235	0.0
38	9.081942736534657E-5	0.0	0.0	7.033692191163996	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	20	7.856336E-4	44.000004	1
ATCCCGT	20	7.856336E-4	44.000004	21
TATGCGA	50	2.7284841E-11	44.0	2
TACGATG	35	1.4460056E-7	44.0	1
GTAACGA	45	4.802132E-10	44.0	2
CGTTTTT	5285	0.0	42.58467	1
CGTAAGA	105	0.0	41.904762	2
TACCGGT	60	3.6379788E-12	40.333332	40
TTTAGCG	50	1.3478711E-9	39.600002	1
ACCGGTA	50	1.3478711E-9	39.600002	41
GCGTAAG	95	0.0	39.368423	1
GTTTTTA	3040	0.0	39.151318	2
TGTACGA	90	0.0	39.11111	2
AGACTTA	700	0.0	38.028572	8
CGACGGT	280	0.0	37.714287	28
CTAACGA	35	7.2899293E-6	37.714287	2
TACGTAG	35	7.2899293E-6	37.714287	1
ACGGTCT	285	0.0	37.05263	30
TTAGCGA	120	0.0	36.666664	2
TACGAGT	30	1.3010597E-4	36.666664	4
>>END_MODULE