FastQCFastQC Report
Thu 26 May 2016
SRR1546483_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546483_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1271128
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131771.0366383243858996No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123790.9738594382312403No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCT15830.1245350586250952No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTT13830.1088010019447294No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG551.8189894E-1244.01
AGCGATC351.4462421E-744.08
CGTCGAA207.856798E-444.015
TCGCGCG302.5279296E-644.01
ATCGACG207.856798E-444.01
CGTTTTT148300.043.4956171
CGACGGT1950.042.87179628
TTAGCGA1300.042.307692
GACGGTC1950.041.7435929
GAGCGAT6550.040.97717
CACGACG2000.040.69999726
GCGCGAC2650.040.6792459
GACCGAT3250.040.6153879
ATTGCGA1150.040.1739162
GTTTTTA81600.040.171572
GCGATAT1050.039.809529
ACGACGG2050.039.70731727
TCGATAG501.3478711E-939.61
TATGCGA950.039.368422
CGAGGTT1800.039.1111156
Produced by FastQC (version 0.11.5)