##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546483_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1271128
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62935440018629	31.0	31.0	34.0	30.0	34.0
2	31.997930184843696	31.0	31.0	34.0	30.0	34.0
3	32.03428136269518	33.0	31.0	34.0	30.0	34.0
4	35.747557287700374	37.0	35.0	37.0	35.0	37.0
5	35.70539788282533	37.0	35.0	37.0	33.0	37.0
6	35.750069229849395	37.0	35.0	37.0	35.0	37.0
7	36.12507316336357	37.0	35.0	37.0	35.0	37.0
8	18.330120176724925	28.0	0.0	37.0	0.0	37.0
9	27.902540892813313	27.0	17.0	39.0	17.0	39.0
10	33.73211037755443	32.0	32.0	39.0	27.0	39.0
11	35.85853981660384	37.0	35.0	39.0	32.0	39.0
12	36.6901177536802	38.0	35.0	39.0	33.0	39.0
13	36.92394943703545	39.0	37.0	39.0	33.0	39.0
14	38.14258988866582	40.0	37.0	41.0	33.0	41.0
15	38.355066523591645	40.0	37.0	41.0	34.0	41.0
16	38.404480115299165	40.0	37.0	41.0	34.0	41.0
17	38.3615544618638	40.0	37.0	41.0	34.0	41.0
18	38.268640923652065	40.0	37.0	41.0	34.0	41.0
19	38.22384685098589	40.0	37.0	41.0	34.0	41.0
20	38.07944911920751	40.0	36.0	41.0	34.0	41.0
21	37.95813246187638	40.0	36.0	41.0	33.0	41.0
22	37.961475948920956	40.0	36.0	41.0	33.0	41.0
23	37.76891154942697	39.0	36.0	41.0	33.0	41.0
24	37.523351700222165	39.0	35.0	41.0	33.0	41.0
25	37.3855245105135	39.0	35.0	41.0	33.0	41.0
26	37.33528488083026	39.0	35.0	41.0	33.0	41.0
27	37.193344022002506	39.0	35.0	41.0	33.0	41.0
28	37.14856961690719	39.0	35.0	41.0	33.0	41.0
29	37.03007800945302	39.0	35.0	41.0	32.0	41.0
30	36.87136779301534	39.0	35.0	41.0	32.0	41.0
31	36.75408377441139	39.0	35.0	41.0	31.0	41.0
32	36.350978028963254	39.0	35.0	41.0	30.0	41.0
33	36.032545896243334	39.0	35.0	41.0	29.0	41.0
34	35.74648973195461	39.0	35.0	41.0	25.0	41.0
35	35.44393562253369	39.0	35.0	41.0	24.0	41.0
36	35.29518663738034	39.0	35.0	41.0	23.0	41.0
37	35.227732376283114	39.0	35.0	41.0	23.0	41.0
38	35.15533762138825	39.0	35.0	41.0	22.0	41.0
39	35.07652179796212	39.0	35.0	41.0	22.0	41.0
40	35.01692827158241	39.0	35.0	41.0	21.0	41.0
41	34.98938108514642	39.0	35.0	41.0	21.0	41.0
42	34.82507977166737	39.0	35.0	40.0	20.0	41.0
43	34.832193925395394	39.0	35.0	40.0	20.0	41.0
44	34.6014185825503	38.0	34.0	40.0	18.0	41.0
45	34.51586464935081	38.0	34.0	40.0	19.0	41.0
46	34.5070881925345	38.0	34.0	40.0	19.0	41.0
47	34.392038409979165	38.0	34.0	40.0	18.0	41.0
48	34.36126416851804	38.0	34.0	40.0	18.0	41.0
49	34.33227888930147	38.0	34.0	40.0	18.0	41.0
50	33.77062341479379	38.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	3.0
14	12.0
15	13.0
16	44.0
17	153.0
18	362.0
19	854.0
20	1686.0
21	2977.0
22	5039.0
23	7937.0
24	12383.0
25	19710.0
26	27176.0
27	29815.0
28	28203.0
29	26863.0
30	28662.0
31	34633.0
32	43791.0
33	57041.0
34	82802.0
35	124943.0
36	124765.0
37	190965.0
38	218026.0
39	202267.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.503873724754705	22.152293081420595	26.844818145772887	21.49901504805181
2	37.98217016696981	22.780239283533994	27.245092547721395	11.992498001774802
3	26.053001743333482	23.00759640256528	38.770446406656134	12.168955447445104
4	23.418491292773034	25.298081703809526	38.19819876519123	13.085228238226204
5	20.960202277032682	29.648312365080464	36.46312566476389	12.92835969312296
6	18.441337143072925	37.72861584356571	33.550594432661384	10.279452580699978
7	80.72562322598512	2.625384697685835	14.866795476144024	1.7821966001850325
8	41.26673316927957	50.29344015708882	7.186215707623465	1.253610966008144
9	76.55373809718611	4.505211119572537	16.068720065957166	2.872330717284176
10	35.95892781844157	33.67906300545657	20.7887797295001	9.573229446601758
11	26.97745624358837	22.5653120692802	36.516306776343534	13.9409249107879
12	26.205543422849626	21.169858582298556	35.63512093195965	16.98947706289217
13	20.528538432006847	26.870858009578896	36.50600096921789	16.094602589196366
14	16.140703375269837	30.262648608165343	36.25748154395151	17.3391664726133
15	15.330320785947599	25.20485741797836	43.589787967852175	15.875033828221863
16	18.081813947926566	23.855583387353594	41.868796848153764	16.193805816566073
17	18.219958965580176	23.81026930411414	35.816691946051066	22.153079784254615
18	19.015394201056072	24.77287889181892	37.91058020907415	18.301146698050864
19	21.828250184088464	26.448870609411486	33.80304737209786	17.9198318344022
20	23.577641276094933	25.48256351838682	33.10217381727096	17.83762138824729
21	20.9223618707164	26.638623332976696	35.0052079727612	17.4338068235457
22	20.040074642364893	23.389304617630955	35.11951589454406	21.451104845460094
23	17.59248478516719	27.288361203592398	35.19527537746002	19.92387863378039
24	17.979306568653982	24.693107224449466	39.2725201553266	18.05506605156994
25	18.229163388738193	26.625328055081788	36.704014072540296	18.44149448363973
26	17.63858557124066	29.946866090590408	33.869366421005594	18.54518191716334
27	18.118946321692228	27.797672618335838	35.2678093787565	18.815571681215424
28	17.64708196184806	25.955057240498203	38.69248415580493	17.705376641848815
29	17.957121548734666	23.67291098929455	38.279150486811716	20.090816975159072
30	18.902502344374444	25.659414315474134	36.435669735856656	19.00241360429477
31	22.39286680806339	24.457017703960577	33.24031883492457	19.909796653051462
32	23.0900428595704	24.78405007206198	33.58615340075901	18.539753667608615
33	21.646836510563848	25.315939858141746	33.36863006715296	19.668593564141457
34	19.245347439439616	25.688600990616205	34.2738890182578	20.792162551686378
35	19.16337300413491	25.275031310772793	34.65740664984172	20.90418903525058
36	22.00305555380733	25.604423787376252	32.93909031977897	19.453430339037453
37	19.29538173968318	27.471426953068455	32.94695734811915	20.286233959129216
38	19.181624509884134	28.143192503036673	32.028953811103214	20.646229175975982
39	19.144334795551668	27.525709448615714	31.612866682190937	21.71708907364168
40	21.250731633635635	25.898021285031874	31.783030505189092	21.068216576143396
41	18.80125368963629	25.41939128081515	31.718678213366395	24.060676816182163
42	20.590058593627077	26.645703658482862	30.154555638771235	22.609682109118832
43	20.250517650464783	26.900280695571176	31.038494943074184	21.810706710889853
44	20.516423208362966	28.225560289758388	31.18490034048499	20.07311616139366
45	19.25628260883247	29.357625667910707	29.96165610386995	21.424435619386877
46	21.033208300029578	28.381091440043804	29.96794972654209	20.61775053338452
47	20.295281041720425	26.41299696018025	31.009858959915913	22.28186303818341
48	20.28387385062716	25.048854245992537	32.349535215965666	22.317736687414644
49	20.284267202044166	24.450802751571832	32.68419860155704	22.580731444826956
50	18.907615912795563	27.01277920083579	32.737930405120494	21.341674481248152
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	474.0
1	888.0
2	1302.0
3	20227.5
4	39153.0
5	26773.5
6	14394.0
7	14368.0
8	14342.0
9	14529.5
10	14717.0
11	14710.0
12	14703.0
13	14269.0
14	13835.0
15	12967.0
16	12099.0
17	11376.5
18	10654.0
19	10256.0
20	9858.0
21	9876.5
22	9895.0
23	10494.0
24	11093.0
25	12622.0
26	14151.0
27	16258.5
28	18366.0
29	21297.5
30	24229.0
31	27951.0
32	31673.0
33	35198.0
34	38723.0
35	42426.5
36	46130.0
37	50864.0
38	55598.0
39	63778.0
40	71958.0
41	80603.5
42	89249.0
43	95981.5
44	102714.0
45	107827.5
46	112941.0
47	114211.0
48	115481.0
49	106949.5
50	98418.0
51	85845.0
52	73272.0
53	64250.0
54	55228.0
55	49378.5
56	43529.0
57	39605.0
58	35681.0
59	32086.0
60	28491.0
61	24559.0
62	20627.0
63	16268.5
64	11910.0
65	9208.0
66	6506.0
67	5285.0
68	4064.0
69	3337.5
70	2611.0
71	2108.0
72	1605.0
73	1162.0
74	719.0
75	542.0
76	365.0
77	284.5
78	204.0
79	150.0
80	96.0
81	67.5
82	39.0
83	31.0
84	23.0
85	14.0
86	5.0
87	2.5
88	0.0
89	1.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1271128.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.527322866984264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.55363109548838	29.299034179404472
2	8.964729713826825	7.44562450482668
3	4.816251877306909	6.000181401529292
4	3.2755097938100044	5.440926110460683
5	2.4243076301659077	5.033750284339656
6	1.9257002509280954	4.798150563919417
7	1.4984089226426824	4.35574377821469
8	1.2453560880086005	4.13730434808781
9	0.9846656041517475	3.6801473813661367
>10	4.274674166582308	25.478677490436937
>50	0.023563995863575552	0.6500008970404637
>100	0.010713746165449834	0.916679271981898
>500	0.001530532344310012	0.4131329203334072
>1k	5.739496291162546E-4	0.3202574618969163
>5k	0.0	0.0
>10k+	3.82633086077503E-4	2.0303894061616456
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13177	1.0366383243858996	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12379	0.9738594382312403	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCT	1583	0.1245350586250952	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTT	1383	0.1088010019447294	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04248195303698762	0.0
2	0.0	0.0	0.0	0.1394824124714427	0.0
3	0.0	0.0	0.0	0.2515088960356471	0.0
4	0.0	0.0	0.0	0.38312428016690686	0.0
5	0.0	0.0	0.0	0.7221932016287895	0.0
6	0.0	0.0	0.0	1.3347200281954297	0.0
7	0.0	0.0	0.0	1.7345224084435242	0.0
8	0.0	0.0	0.0	2.468358812015784	0.0
9	0.0	0.0	0.0	3.318941916156359	0.0
10	0.0	0.0	0.0	4.189821953414605	0.0
11	0.0	0.0	0.0	4.69504251342115	0.0
12	7.867028340182893E-5	0.0	0.0	5.131033224034086	0.0
13	7.867028340182893E-5	0.0	0.0	5.404648469705648	0.0
14	7.867028340182893E-5	0.0	0.0	5.566001220962798	0.0
15	7.867028340182893E-5	0.0	0.0	5.698167297077871	0.0
16	7.867028340182893E-5	0.0	0.0	5.887290658375868	0.0
17	7.867028340182893E-5	0.0	0.0	6.089394616435166	0.0
18	7.867028340182893E-5	0.0	0.0	6.406671869394742	0.0
19	7.867028340182893E-5	0.0	0.0	6.578959790044748	0.0
20	1.5734056680365786E-4	0.0	0.0	6.779254331585804	0.0
21	1.5734056680365786E-4	0.0	0.0	6.97435663442234	0.0
22	1.5734056680365786E-4	0.0	0.0	7.184327620821821	0.0
23	1.5734056680365786E-4	0.0	0.0	7.420731822444317	0.0
24	1.5734056680365786E-4	0.0	0.0	7.612136621960967	0.0
25	1.5734056680365786E-4	0.0	0.0	7.772309318967091	0.0
26	1.5734056680365786E-4	0.0	0.0	7.939641011762781	0.0
27	1.5734056680365786E-4	0.0	0.0	8.11594111686628	0.0
28	1.5734056680365786E-4	0.0	0.0	8.307503256949733	0.0
29	1.5734056680365786E-4	0.0	0.0	8.502054867802455	0.0
30	1.5734056680365786E-4	0.0	0.0	8.751124985052646	0.0
31	1.5734056680365786E-4	0.0	0.0	8.958814533233474	0.0
32	2.3601085020548678E-4	0.0	0.0	9.170909617284805	0.0
33	2.3601085020548678E-4	0.0	0.0	9.375295013562758	0.0
34	2.3601085020548678E-4	0.0	0.0	9.592661006602011	0.0
35	2.3601085020548678E-4	0.0	0.0	9.820568817617108	0.0
36	2.3601085020548678E-4	0.0	0.0	10.035417361587504	0.0
37	2.3601085020548678E-4	0.0	0.0	10.254199419727989	0.0
38	2.3601085020548678E-4	0.0	0.0	10.493199740702746	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	55	1.8189894E-12	44.0	1
AGCGATC	35	1.4462421E-7	44.0	8
CGTCGAA	20	7.856798E-4	44.0	15
TCGCGCG	30	2.5279296E-6	44.0	1
ATCGACG	20	7.856798E-4	44.0	1
CGTTTTT	14830	0.0	43.495617	1
CGACGGT	195	0.0	42.871796	28
TTAGCGA	130	0.0	42.30769	2
GACGGTC	195	0.0	41.74359	29
GAGCGAT	655	0.0	40.9771	7
CACGACG	200	0.0	40.699997	26
GCGCGAC	265	0.0	40.679245	9
GACCGAT	325	0.0	40.615387	9
ATTGCGA	115	0.0	40.173916	2
GTTTTTA	8160	0.0	40.17157	2
GCGATAT	105	0.0	39.80952	9
ACGACGG	205	0.0	39.707317	27
TCGATAG	50	1.3478711E-9	39.6	1
TATGCGA	95	0.0	39.36842	2
CGAGGTT	180	0.0	39.111115	6
>>END_MODULE