FastQCFastQC Report
Thu 26 May 2016
SRR1546482_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546482_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1344928
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75630.5623349354017464No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70160.5216636132194437No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACA302.5280406E-644.040
GCCGGAT302.5280406E-644.019
GACCCCG254.443246E-544.021
AACGCGA351.446333E-744.02
CCGGTCA207.856961E-444.031
TCGACAT302.5280406E-644.041
CGTTTTT103350.043.2975351
CGGTCTA1950.042.8717931
GAGCGAT8550.042.1988337
GTTGCGA1000.041.82
GCGAGGT2200.040.0000045
TCACGAC2050.039.70731725
TATACGA452.3514076E-839.111112
CACGACG2100.038.76190626
GCCGATC404.1252315E-738.59
TGCGCGA404.1252315E-738.52
CGACGGT2200.038.00000428
GTTTTTA60700.037.8747942
ACGACGG2150.037.86046627
TCGATTG357.2911334E-637.7142871
Produced by FastQC (version 0.11.5)