##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546482_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1344928
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.606048056104118	31.0	31.0	34.0	30.0	34.0
2	31.97141557020153	31.0	31.0	34.0	30.0	34.0
3	32.04471466130529	33.0	31.0	34.0	30.0	34.0
4	35.7368989269314	37.0	35.0	37.0	35.0	37.0
5	35.69017746674915	37.0	35.0	37.0	33.0	37.0
6	35.73986860263152	37.0	35.0	37.0	33.0	37.0
7	36.10985941254848	37.0	35.0	37.0	35.0	37.0
8	18.148311285065073	18.0	0.0	37.0	0.0	37.0
9	27.799424950629327	19.0	17.0	39.0	17.0	39.0
10	33.65852967593804	32.0	32.0	39.0	27.0	39.0
11	35.81908548264294	37.0	35.0	39.0	32.0	39.0
12	36.56496704656309	37.0	35.0	39.0	33.0	39.0
13	36.744526101025485	39.0	35.0	39.0	33.0	39.0
14	37.93275476456732	40.0	37.0	41.0	33.0	41.0
15	38.187157230721645	40.0	37.0	41.0	33.0	41.0
16	38.2642037343263	40.0	37.0	41.0	34.0	41.0
17	38.22451610792548	40.0	37.0	41.0	34.0	41.0
18	38.17314904589688	40.0	37.0	41.0	34.0	41.0
19	38.15266244735778	40.0	37.0	41.0	34.0	41.0
20	38.035105968497945	40.0	36.0	41.0	34.0	41.0
21	37.90228324490233	40.0	36.0	41.0	33.0	41.0
22	37.90812816745581	40.0	36.0	41.0	33.0	41.0
23	37.760325459801564	39.0	36.0	41.0	33.0	41.0
24	37.58967097123415	39.0	35.0	41.0	33.0	41.0
25	37.460368882200385	39.0	35.0	41.0	33.0	41.0
26	37.41049632396678	39.0	35.0	41.0	33.0	41.0
27	37.25758404910895	39.0	35.0	41.0	33.0	41.0
28	37.248231132075475	39.0	35.0	41.0	33.0	41.0
29	37.15373090604107	39.0	35.0	41.0	32.0	41.0
30	37.04439122391682	39.0	35.0	41.0	32.0	41.0
31	36.98072015751029	39.0	35.0	41.0	32.0	41.0
32	36.67756043446192	39.0	35.0	41.0	31.0	41.0
33	36.47649465250184	39.0	35.0	41.0	30.0	41.0
34	36.284839039710675	39.0	35.0	41.0	30.0	41.0
35	36.074699166052014	39.0	35.0	41.0	29.0	41.0
36	35.932084840229365	39.0	35.0	41.0	27.0	41.0
37	35.860947203121654	39.0	35.0	41.0	27.0	41.0
38	35.782646357277116	39.0	35.0	41.0	27.0	41.0
39	35.73231280782317	39.0	35.0	41.0	27.0	41.0
40	35.67081360489186	39.0	35.0	41.0	26.0	41.0
41	35.68407974255871	39.0	35.0	41.0	26.0	41.0
42	35.53492752028362	39.0	35.0	41.0	25.0	41.0
43	35.54439791572486	39.0	35.0	40.0	26.0	41.0
44	35.31174456922601	38.0	35.0	40.0	24.0	41.0
45	35.22598756215946	38.0	35.0	40.0	24.0	41.0
46	35.23447128768232	38.0	35.0	40.0	24.0	41.0
47	35.13422428561231	38.0	35.0	40.0	24.0	41.0
48	35.11881230816817	38.0	35.0	40.0	24.0	41.0
49	35.09204433248471	38.0	34.0	40.0	24.0	41.0
50	34.48526017749649	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	1.0
14	5.0
15	21.0
16	48.0
17	137.0
18	321.0
19	783.0
20	1531.0
21	2665.0
22	4338.0
23	6786.0
24	10700.0
25	16251.0
26	22205.0
27	24599.0
28	24547.0
29	25206.0
30	28919.0
31	36110.0
32	46574.0
33	62076.0
34	93693.0
35	146225.0
36	134413.0
37	208212.0
38	238060.0
39	210501.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.68407528135335	23.601412120202717	28.273260724737682	16.44125187370625
2	34.66274774560423	23.6503366722977	29.168773346974707	12.518142235123367
3	27.369048752052155	23.81398855552119	35.85098979276214	12.965972899664518
4	24.352530395679175	26.341633158057533	35.46055997049656	13.845276475766733
5	22.193009588617382	31.177133645815985	33.23389802279378	13.395958742772848
6	19.191733683884937	39.63543029812748	30.64877822455923	10.524057793428348
7	84.91004722929407	2.9362910133479265	10.56904161412358	1.5846201432344336
8	43.1363612029789	50.83952449499155	4.996550001189655	1.0275643008398962
9	81.36182754764567	4.522695638725642	11.540022960336909	2.575453853291775
10	40.73794284898523	33.04407373480216	16.66825287301625	9.549730543196365
11	32.194362820909376	22.129363058840323	31.523620595303242	14.152653524947059
12	29.812450926741057	22.34677246663018	31.423317828166265	16.41745877846249
13	20.761854909705203	31.298032311023338	32.29169145114088	15.648421328130576
14	15.995428751576293	34.09386970901044	33.33033441195365	16.58036712745961
15	16.013645340122295	24.840437555021534	43.85305384377454	15.292863261081635
16	17.85277724904233	23.124732327678508	41.48697922862785	17.535511194651313
17	18.608877203835448	22.533473910871066	32.60196828380404	26.255680601489445
18	20.968854838325917	23.9963031478265	35.617073925146926	19.41776808870066
19	24.276318137476505	25.579213162340288	30.653462490185348	19.491006209997856
20	25.65609460134669	25.58887910728307	29.681960670013563	19.073065621356683
21	21.864590520830856	26.8993581812558	32.57854695567347	18.657504342239882
22	21.526579861524187	23.811757952842086	31.855236860263155	22.806425325370576
23	18.557647695638728	28.173552784981798	31.50874991077589	21.76004960860358
24	18.770447191225106	24.940591615313238	37.661198220276475	18.627762973185185
25	17.873001379999522	27.19982036213091	35.39884662970806	19.528331628161506
26	17.99256168359942	31.63143305812653	31.05155071498251	19.324454543291537
27	19.0589384710557	28.760201289585762	32.92250588879107	19.258354350567465
28	17.52465559494635	26.949026267577146	37.771315639201504	17.755002498275
29	17.767419519855338	24.735376168835803	36.8922351233672	20.60496918794166
30	19.948428466059152	27.28971365009874	33.321412001237235	19.440445882604866
31	25.471846820052818	25.44374122629613	29.170409122272716	19.914002831378333
32	25.32380915558305	27.030666325632303	29.272496371552975	18.373028147231672
33	24.740283494729827	26.59064277046801	28.96779604558757	19.70127768921459
34	19.925601965309667	27.21513716719408	31.007682195626828	21.851578671869422
35	20.836654452877777	26.53182921316234	31.434768255252326	21.196748078707557
36	25.062679935282784	26.83757048704466	28.770239001641723	19.329510576030838
37	20.626382973661045	29.46574091698589	30.39434081229627	19.513535297056794
38	20.174685931142783	31.195722001475172	27.71285897832449	20.916733089057555
39	20.461987556211188	29.066463037426537	29.29383580384972	21.17771360251255
40	22.032331842299367	26.99319220062338	28.241140046158602	22.73333591091865
41	18.57355932808299	25.97135311332651	29.33577113421685	26.119316424373647
42	21.597959147255466	27.422806276618527	27.46481596040829	23.51441861571772
43	21.65915201408551	27.289118822717644	28.435722953198983	22.616006209997856
44	20.74460491565348	29.071072949630018	29.265730210093032	20.918591924623474
45	19.531231411644338	31.4059934806919	27.177588688762523	21.885186418901235
46	21.87842025744129	29.482767850769708	28.352149706155274	20.28666218563373
47	21.31891075209974	26.428701015965167	29.218218373028147	23.034169858906946
48	21.585839539365676	25.249083965833112	30.831985057936183	22.333091436865022
49	20.93703157343739	24.23750565086012	31.495143234433364	23.330319541269123
50	19.509371505389137	29.00080896523829	29.701813033857576	21.788006495515003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	311.0
1	692.5
2	1074.0
3	12220.0
4	23366.0
5	16538.5
6	9711.0
7	9816.0
8	9921.0
9	10268.5
10	10616.0
11	10800.5
12	10985.0
13	10706.5
14	10428.0
15	9887.0
16	9346.0
17	8892.5
18	8439.0
19	8241.5
20	8044.0
21	8087.0
22	8130.0
23	8752.0
24	9374.0
25	11159.5
26	12945.0
27	15227.5
28	17510.0
29	20167.5
30	22825.0
31	26636.5
32	30448.0
33	34152.0
34	37856.0
35	42675.5
36	47495.0
37	54069.5
38	60644.0
39	71799.0
40	82954.0
41	94949.5
42	106945.0
43	114034.0
44	121123.0
45	123673.0
46	126223.0
47	125244.5
48	124266.0
49	116784.5
50	109303.0
51	97053.0
52	84803.0
53	75164.0
54	65525.0
55	58313.0
56	51101.0
57	45322.0
58	39543.0
59	34699.5
60	29856.0
61	25773.0
62	21690.0
63	17588.5
64	13487.0
65	10734.5
66	7982.0
67	6200.0
68	4418.0
69	3577.0
70	2736.0
71	2278.5
72	1821.0
73	1370.0
74	919.0
75	680.0
76	441.0
77	327.5
78	214.0
79	137.5
80	61.0
81	49.5
82	38.0
83	21.5
84	5.0
85	4.0
86	3.0
87	2.0
88	1.0
89	0.5
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1344928.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.911503900482565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.96145944258052	25.122827720140677
2	7.448958122201045	5.201086610755061
3	3.8032917808400333	3.9833590752441026
4	2.578609491165328	3.600925412345589
5	2.0166863304272464	3.520277634538034
6	1.5715618372982953	3.2919352327613396
7	1.32776406066251	3.244796812791786
8	1.1301173304341872	3.156327647156485
9	0.9905780430387234	3.1124312291950904
>10	7.087241113207667	42.007139412533704
>50	0.06914424577292293	1.546302352475536
>100	0.013301008852898647	0.8904440769129034
>500	6.43596759325121E-4	0.1302449020504863
>1k	2.1453225310837364E-4	0.09998674194215021
>5k	4.290645062167473E-4	1.0919151391570097
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7563	0.5623349354017464	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7016	0.5216636132194437	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.035094815484546384	0.0
2	0.0	0.0	0.0	0.11985771729044231	0.0
3	0.0	0.0	0.0	0.21220466820528683	0.0
4	0.0	0.0	0.0	0.319273596802208	0.0
5	0.0	0.0	0.0	0.5569071355492636	0.0
6	0.0	0.0	0.0	0.9278563610840134	0.0
7	0.0	0.0	0.0	1.1820707130790644	0.0
8	0.0	0.0	0.0	1.7031394989174142	0.0
9	0.0	0.0	0.0	2.2538753003878274	0.0
10	0.0	0.0	0.0	2.906252230602679	0.0
11	0.0	0.0	0.0	3.3415171667182184	0.0
12	0.0	0.0	0.0	3.7309060410668824	0.0
13	0.0	0.0	0.0	3.9365676080801353	0.0
14	0.0	0.0	0.0	4.0390266244735775	0.0
15	0.0	0.0	0.0	4.140965166908563	0.0
16	0.0	0.0	0.0	4.340381046420329	0.0
17	0.0	0.0	0.0	4.571545837397987	0.0
18	0.0	0.0	0.0	4.922122225130267	0.0
19	0.0	0.0	0.0	5.090904494515692	0.0
20	0.0	0.0	0.0	5.285933522091889	0.0
21	0.0	0.0	0.0	5.483787979728283	0.0
22	0.0	0.0	0.0	5.700751266982322	0.0
23	0.0	0.0	0.0	5.9524376026077235	0.0
24	0.0	0.0	0.0	6.142336244021985	0.0
25	0.0	0.0	0.0	6.30925967784149	0.0
26	0.0	0.0	0.0	6.473655095291346	0.0
27	7.435342263675082E-5	0.0	0.0	6.640206761997669	0.0
28	7.435342263675082E-5	0.0	0.0	6.817837088676866	0.0
29	7.435342263675082E-5	0.0	0.0	7.009668919079683	0.0
30	7.435342263675082E-5	0.0	0.0	7.255630041162055	0.0
31	7.435342263675082E-5	0.0	0.0	7.460399367103666	0.0
32	7.435342263675082E-5	0.0	0.0	7.665094339622642	0.0
33	7.435342263675082E-5	0.0	0.0	7.859825953508292	0.0
34	7.435342263675082E-5	0.0	0.0	8.070989673796664	0.0
35	7.435342263675082E-5	0.0	0.0	8.301336577125317	0.0
36	7.435342263675082E-5	0.0	0.0	8.514656546670157	0.0
37	7.435342263675082E-5	0.0	0.0	8.733404316067476	0.0
38	7.435342263675082E-5	0.0	0.0	8.960479668800113	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACA	30	2.5280406E-6	44.0	40
GCCGGAT	30	2.5280406E-6	44.0	19
GACCCCG	25	4.443246E-5	44.0	21
AACGCGA	35	1.446333E-7	44.0	2
CCGGTCA	20	7.856961E-4	44.0	31
TCGACAT	30	2.5280406E-6	44.0	41
CGTTTTT	10335	0.0	43.297535	1
CGGTCTA	195	0.0	42.87179	31
GAGCGAT	855	0.0	42.198833	7
GTTGCGA	100	0.0	41.8	2
GCGAGGT	220	0.0	40.000004	5
TCACGAC	205	0.0	39.707317	25
TATACGA	45	2.3514076E-8	39.11111	2
CACGACG	210	0.0	38.761906	26
GCCGATC	40	4.1252315E-7	38.5	9
TGCGCGA	40	4.1252315E-7	38.5	2
CGACGGT	220	0.0	38.000004	28
GTTTTTA	6070	0.0	37.874794	2
ACGACGG	215	0.0	37.860466	27
TCGATTG	35	7.2911334E-6	37.714287	1
>>END_MODULE