FastQCFastQC Report
Thu 26 May 2016
SRR1546481_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546481_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences842007
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74380.8833655777208503No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70720.8398980055985282No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGA502.7284841E-1144.02
GATGCGT207.8552816E-444.011
GCGATAC254.4418233E-544.09
GTAAGCG302.5269073E-644.01
GATTCGA207.8552816E-444.09
CGTTTTT89200.043.4080731
TGTAGCG650.040.6153831
GTTTTTA47800.040.548122
GAGCGAT5050.039.207927
TACGAGT452.3495886E-839.111114
AGCGATA900.039.111118
TAATGCG452.3495886E-839.111111
TACCGGG452.3495886E-839.111113
TAGCGGA800.038.52
TAAGCGA357.2878865E-637.7142872
GCGCGAC3800.037.631589
CGACGGT1300.037.23076628
CGGTCTA1250.036.9631
TCTACGA301.3007999E-436.6666642
ATGCGCC301.3007999E-436.66666413
Produced by FastQC (version 0.11.5)