##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546481_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	842007
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50425827813783	31.0	31.0	34.0	30.0	34.0
2	31.890947462432024	31.0	31.0	34.0	30.0	34.0
3	31.957628618289398	31.0	31.0	34.0	30.0	34.0
4	35.69204412789917	37.0	35.0	37.0	33.0	37.0
5	35.6562760167077	37.0	35.0	37.0	33.0	37.0
6	35.70463190923591	37.0	35.0	37.0	33.0	37.0
7	36.00571610449794	37.0	35.0	37.0	35.0	37.0
8	18.11598953452881	21.0	0.0	37.0	0.0	37.0
9	27.726939324732456	19.0	17.0	39.0	17.0	39.0
10	33.58444882287202	32.0	32.0	39.0	27.0	39.0
11	35.811245037155274	37.0	35.0	39.0	32.0	39.0
12	36.59953777106366	37.0	35.0	39.0	33.0	39.0
13	36.825130907462764	39.0	35.0	39.0	33.0	39.0
14	38.005903751393994	40.0	37.0	41.0	33.0	41.0
15	38.20419426441823	40.0	37.0	41.0	33.0	41.0
16	38.26059165778907	40.0	37.0	41.0	33.0	41.0
17	38.19015043817925	40.0	37.0	41.0	33.0	41.0
18	38.115641556424116	40.0	37.0	41.0	33.0	41.0
19	38.08618218138329	40.0	37.0	41.0	34.0	41.0
20	37.919132501273744	40.0	36.0	41.0	33.0	41.0
21	37.81701221011227	40.0	36.0	41.0	33.0	41.0
22	37.826687901644526	40.0	36.0	41.0	33.0	41.0
23	37.63026554411068	39.0	35.0	41.0	33.0	41.0
24	37.385420786288	39.0	35.0	41.0	33.0	41.0
25	37.24963925478054	39.0	35.0	41.0	33.0	41.0
26	37.2211489928231	39.0	35.0	41.0	33.0	41.0
27	37.08681756802497	39.0	35.0	41.0	32.0	41.0
28	37.0380840064275	39.0	35.0	41.0	32.0	41.0
29	36.89636903256149	39.0	35.0	40.0	32.0	41.0
30	36.74677645197724	39.0	35.0	40.0	31.0	41.0
31	36.63705052333294	39.0	35.0	41.0	31.0	41.0
32	36.229437522490905	39.0	35.0	41.0	30.0	41.0
33	35.91848167533049	39.0	35.0	41.0	27.0	41.0
34	35.583963078691745	39.0	35.0	41.0	25.0	41.0
35	35.28194777478097	39.0	35.0	41.0	23.0	41.0
36	35.12352035078093	39.0	35.0	41.0	23.0	41.0
37	35.02528601306165	39.0	35.0	41.0	22.0	41.0
38	34.95648017178004	39.0	35.0	40.0	22.0	41.0
39	34.88029671962347	38.0	35.0	40.0	21.0	41.0
40	34.80853128299408	38.0	34.0	40.0	21.0	41.0
41	34.784664498038616	38.0	34.0	40.0	21.0	41.0
42	34.62613256184331	38.0	34.0	40.0	20.0	41.0
43	34.635561224550386	38.0	34.0	40.0	20.0	41.0
44	34.39596345398554	38.0	34.0	40.0	18.0	41.0
45	34.30735492697804	38.0	34.0	40.0	19.0	41.0
46	34.307967748486654	38.0	34.0	40.0	20.0	41.0
47	34.18176570978626	38.0	33.0	40.0	19.0	41.0
48	34.16467084002865	38.0	34.0	40.0	19.0	41.0
49	34.11258813762831	38.0	33.0	40.0	19.0	41.0
50	33.534674889876214	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	9.0
15	15.0
16	43.0
17	109.0
18	254.0
19	617.0
20	1248.0
21	2262.0
22	3627.0
23	5747.0
24	8755.0
25	13315.0
26	17736.0
27	20119.0
28	19435.0
29	18809.0
30	20343.0
31	24067.0
32	30684.0
33	39637.0
34	57835.0
35	84778.0
36	85250.0
37	127296.0
38	138437.0
39	121577.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.08726293249344	21.458728965436155	26.040876144735137	20.41313195733527
2	38.30668866173322	22.82843254272233	26.65346012562841	12.211418669916046
3	27.40879826414745	23.597903580374037	36.766202656272455	12.227095499206063
4	24.049325005611593	24.888985483493606	37.11334941395974	13.94834009693506
5	21.81157638831981	29.441560462086418	35.00695362390099	13.739909525692779
6	18.916469815571606	38.18044268040527	32.44961146403771	10.453476039985416
7	80.20657785505347	3.0156518888797836	14.715673385138128	2.0620968709286265
8	40.48838073792736	51.11216415065433	6.964550175948656	1.4349049354696577
9	75.62644966134486	4.556494185915319	15.973145116370766	3.843911036369056
10	36.66109664171438	31.1709997660352	20.986761392720013	11.181142199530408
11	29.87766134960873	21.910387918390228	33.273476348771446	14.938474383229593
12	27.896086374578832	20.07714900232421	34.37999921615854	17.64676540693842
13	22.625227581243387	24.42034329880868	35.61644974447956	17.337979375468375
14	17.628832064341506	28.631115893335803	35.834975243673746	17.905076798648942
15	17.008765960378003	23.444341911646816	42.30523024155381	17.241661886421372
16	20.063491158624572	21.778084980291137	40.45928359265422	17.69914026843007
17	19.784871147151982	22.301121012058093	35.22619170624473	22.687816134545198
18	20.957189192013843	23.308832349374768	36.51264181889224	19.221336639719148
19	23.241612005600903	26.03137503607452	32.15626473414117	18.570748224183408
20	25.04634759568507	24.611196819028823	31.72432058165787	18.618135003628236
21	22.165255158211274	26.60108526413676	33.688912324956924	17.544747252695046
22	21.05386297263562	22.100528855460823	34.73581573549864	22.109792436404923
23	19.603756263308973	26.5501355689442	33.56789195339231	20.278216214354515
24	19.945083591941636	24.270463309687447	36.96418200798806	18.82027109038286
25	20.40232444623382	25.090646514815194	34.4595710011912	20.04745803775978
26	18.700438357400827	27.277801728489194	33.55720320614912	20.46455670796086
27	19.61088209480444	25.04551624867727	34.470972331583944	20.87262932493435
28	18.882622115968157	25.345276226919726	36.971426603341776	18.800675053770338
29	19.85007250533547	24.542551308955865	35.82915581461912	19.778220371089553
30	21.241628632541058	25.176869075910297	34.240926738138754	19.340575553409888
31	25.041121985921734	23.47320152920344	30.85520666692795	20.63046981794688
32	24.89539873183952	25.859880024750385	30.100224819983683	19.144496423426407
33	24.903830965775818	25.489574314702846	29.495360489877164	20.11123422964417
34	21.08711685294778	26.56462475965164	30.9283652036147	21.41989318378588
35	20.98996801689297	27.44454618548302	30.87123978779274	20.69424600983127
36	23.96963445672067	28.01330630267919	28.09287808771186	19.92418115288828
37	21.64982001337281	29.981223434009458	27.99964845897956	20.36930809363818
38	21.28521496852164	30.53561312435645	27.48860757689663	20.690564330225282
39	21.296853826630898	28.59026112609515	27.38183886832295	22.731046178951004
40	22.249933789149022	26.150613949765262	29.458781221533787	22.14067103955193
41	19.692354101569226	25.487555329112467	29.651772491202564	25.168318078115743
42	21.09067976869551	27.647513619245444	27.549058380749802	23.71274823130924
43	20.8972134435937	26.436716084308088	28.91092354339097	23.755146928707244
44	21.43675765165848	26.94645056395018	30.167563927615802	21.449227856775536
45	20.684151081879367	28.032427283858684	29.13051791730948	22.152903716952473
46	22.509076527867343	27.662596629244174	28.800473155211296	21.02785368767718
47	21.32892006836048	26.346930607465257	29.61828108317389	22.70586824100037
48	21.551127247160654	25.146109236621548	30.705920497098006	22.596843019119795
49	21.820246149972625	24.10550031056749	31.0878650652548	22.986388474205082
50	19.972874334773937	25.76475017428596	31.395938513575306	22.866436977364796
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	225.0
1	622.5
2	1020.0
3	12462.0
4	23904.0
5	16610.0
6	9316.0
7	9202.5
8	9089.0
9	9124.0
10	9159.0
11	9101.0
12	9043.0
13	8626.5
14	8210.0
15	7739.0
16	7268.0
17	6790.5
18	6313.0
19	6013.0
20	5713.0
21	5647.5
22	5582.0
23	5937.0
24	6292.0
25	6972.5
26	7653.0
27	8768.5
28	9884.0
29	11565.5
30	13247.0
31	15252.5
32	17258.0
33	19389.0
34	21520.0
35	23836.0
36	26152.0
37	28664.0
38	31176.0
39	35195.5
40	39215.0
41	44423.5
42	49632.0
43	53869.0
44	58106.0
45	62918.5
46	67731.0
47	70146.0
48	72561.0
49	69751.5
50	66942.0
51	59812.0
52	52682.0
53	47565.5
54	42449.0
55	39580.0
56	36711.0
57	34939.5
58	33168.0
59	30538.0
60	27908.0
61	25420.5
62	22933.0
63	19268.0
64	15603.0
65	12626.5
66	9650.0
67	8104.5
68	6559.0
69	5808.5
70	5058.0
71	4257.0
72	3456.0
73	2661.0
74	1866.0
75	1358.0
76	850.0
77	651.0
78	452.0
79	359.5
80	267.0
81	186.0
82	105.0
83	72.0
84	39.0
85	32.5
86	26.0
87	17.5
88	9.0
89	6.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	842007.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.03270258261977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.35573144076568	34.70054683575881
2	11.565609307232783	11.573173813111136
3	6.394527247511646	9.598064397936252
4	4.025814098950222	8.056894378627755
5	2.7264250772832654	6.820520750275488
6	1.8401070840461744	5.523931827375238
7	1.2503234068997686	4.37899414046528
8	0.8469585065873707	3.390049844792458
9	0.5893187615886646	2.6536689292431115
>10	1.371328464286161	9.245461580872565
>50	0.017643977122060304	0.6433453981158987
>100	0.015020522754931927	1.4769105154619198
>500	7.152629827740335E-4	0.20756193249584612
>1k	0.0	0.0
>5k	4.768419885160224E-4	1.730875655468234
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7438	0.8833655777208503	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7072	0.8398980055985282	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03147242243829327	0.0
2	0.0	0.0	0.0	0.1124693737700518	0.0
3	0.0	0.0	0.0	0.21781291604464095	0.0
4	0.0	0.0	0.0	0.32375027761051867	0.0
5	0.0	0.0	0.0	0.5837243633366468	0.0
6	0.0	0.0	0.0	1.0398963429045127	0.0
7	0.0	0.0	0.0	1.3554519142952492	0.0
8	0.0	0.0	0.0	2.0337123088050335	0.0
9	0.0	0.0	0.0	2.8352495881863216	0.0
10	0.0	0.0	0.0	3.7700399165327605	0.0
11	0.0	0.0	0.0	4.251271070193003	0.0
12	0.0	0.0	0.0	4.714925172831105	0.0
13	0.0	0.0	0.0	5.013259984774473	0.0
14	0.0	0.0	0.0	5.186417690114215	0.0
15	0.0	0.0	0.0	5.319670739079366	0.0
16	0.0	0.0	0.0	5.496985179458128	0.0
17	0.0	0.0	0.0	5.673587036687343	0.0
18	0.0	0.0	0.0	5.980472846425267	0.0
19	0.0	0.0	0.0	6.147454831135608	0.0
20	0.0	0.0	0.0	6.320375008758835	0.0
21	0.0	0.0	0.0	6.482843966855382	0.0
22	0.0	0.0	0.0	6.6590895325098245	0.0
23	0.0	0.0	0.0	6.862413257847025	0.0
24	0.0	0.0	0.0	7.030345353423428	0.0
25	0.0	0.0	0.0	7.169061539868434	0.0
26	0.0	0.0	0.0	7.309915475762078	0.0
27	0.0	0.0	0.0	7.485329694408716	0.0
28	0.0	0.0	0.0	7.635803502821235	0.0
29	0.0	0.0	0.0	7.801241557374226	0.0
30	0.0	0.0	0.0	8.015016502238105	0.0
31	0.0	0.0	0.0	8.194587455923763	0.0
32	0.0	0.0	0.0	8.384490865277842	0.0
33	0.0	0.0	0.0	8.565249457546077	0.0
34	0.0	0.0	0.0	8.753015117451518	0.0
35	0.0	0.0	0.0	8.955269968064398	0.0
36	0.0	0.0	0.0	9.15229920891394	0.0
37	0.0	0.0	0.0	9.358116975274552	0.0
38	0.0	0.0	0.0	9.606095911316652	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGA	50	2.7284841E-11	44.0	2
GATGCGT	20	7.8552816E-4	44.0	11
GCGATAC	25	4.4418233E-5	44.0	9
GTAAGCG	30	2.5269073E-6	44.0	1
GATTCGA	20	7.8552816E-4	44.0	9
CGTTTTT	8920	0.0	43.408073	1
TGTAGCG	65	0.0	40.615383	1
GTTTTTA	4780	0.0	40.54812	2
GAGCGAT	505	0.0	39.20792	7
TACGAGT	45	2.3495886E-8	39.11111	4
AGCGATA	90	0.0	39.11111	8
TAATGCG	45	2.3495886E-8	39.11111	1
TACCGGG	45	2.3495886E-8	39.11111	3
TAGCGGA	80	0.0	38.5	2
TAAGCGA	35	7.2878865E-6	37.714287	2
GCGCGAC	380	0.0	37.63158	9
CGACGGT	130	0.0	37.230766	28
CGGTCTA	125	0.0	36.96	31
TCTACGA	30	1.3007999E-4	36.666664	2
ATGCGCC	30	1.3007999E-4	36.666664	13
>>END_MODULE