FastQCFastQC Report
Thu 26 May 2016
SRR1546480_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546480_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1962210
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141000.7185775222835475No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134920.6875920518191223No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCC59280.3021083370281468No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTT51720.2635803507269864No Hit
GAATCTGACTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTT43750.222962883687271No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATG42270.21542036785053587No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTCTCCT20700.10549329582460593No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT184050.043.2469441
CGACGGT1750.042.7428628
CGATGAA33350.041.2293919
GAGCGAT11050.041.0135777
CCGATGA32900.040.99088318
TGTACGA1400.040.8571432
GTTTTTA103200.040.013572
GTTTACG1550.039.7419361
GCGCGAC4650.039.7419329
CGAGGTA1500.039.6000026
CACGACG1900.039.36842326
CGCGCGA452.3523171E-839.111112
TAATGCG404.126523E-738.51
CCACCGA38800.038.10309215
GCTTGCG1100.038.01
ACGAGAT5850.037.9829065
ACCGATG35650.037.89060217
TTAGCGA2150.037.8604662
ACGTGCG357.2928397E-637.7142871
CGCACGA357.2928397E-637.7142872