##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546480_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1962210 Sequences flagged as poor quality 0 Sequence length 50 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.560016511994128 31.0 31.0 34.0 30.0 34.0 2 31.9365057766498 31.0 31.0 34.0 30.0 34.0 3 32.001346441002745 33.0 31.0 34.0 30.0 34.0 4 35.72205880104576 37.0 35.0 37.0 35.0 37.0 5 35.653944786745555 37.0 35.0 37.0 33.0 37.0 6 35.70120578327498 37.0 35.0 37.0 33.0 37.0 7 36.038165130133876 37.0 35.0 37.0 35.0 37.0 8 18.153118677409655 23.0 0.0 37.0 0.0 37.0 9 27.7714225286794 23.0 17.0 39.0 17.0 39.0 10 33.59036290713023 32.0 32.0 39.0 27.0 39.0 11 35.747348143165105 37.0 35.0 39.0 32.0 39.0 12 36.57220990617722 37.0 35.0 39.0 33.0 39.0 13 36.79885078559379 39.0 35.0 39.0 33.0 39.0 14 38.006604797651626 40.0 37.0 41.0 33.0 41.0 15 38.21108036346772 40.0 37.0 41.0 33.0 41.0 16 38.289527114834804 40.0 37.0 41.0 34.0 41.0 17 38.194205003541924 40.0 37.0 41.0 33.0 41.0 18 38.159143007119525 40.0 37.0 41.0 33.0 41.0 19 38.141652014820025 40.0 37.0 41.0 34.0 41.0 20 37.890940317295296 40.0 36.0 41.0 33.0 41.0 21 37.857916838666604 40.0 36.0 41.0 33.0 41.0 22 37.835693427309 40.0 36.0 41.0 33.0 41.0 23 37.58424837300799 39.0 36.0 41.0 33.0 41.0 24 37.453166072948356 39.0 35.0 41.0 33.0 41.0 25 37.39782082447852 39.0 35.0 41.0 33.0 41.0 26 37.3083482399947 39.0 35.0 41.0 33.0 41.0 27 37.10811330081898 39.0 35.0 41.0 32.0 41.0 28 37.08242338995316 39.0 35.0 41.0 32.0 41.0 29 36.94284250921155 39.0 35.0 41.0 32.0 41.0 30 36.82223870024105 39.0 35.0 41.0 31.0 41.0 31 36.75371596312321 39.0 35.0 41.0 31.0 41.0 32 36.309430183313715 39.0 35.0 41.0 30.0 41.0 33 36.02521952288491 39.0 35.0 41.0 27.0 41.0 34 35.75406760744263 39.0 35.0 41.0 25.0 41.0 35 35.456005728235 39.0 35.0 41.0 24.0 41.0 36 35.33031224996305 39.0 35.0 41.0 23.0 41.0 37 35.23823138196217 39.0 35.0 41.0 23.0 41.0 38 35.17258193567457 39.0 35.0 41.0 23.0 41.0 39 35.094954158831115 39.0 35.0 41.0 22.0 41.0 40 35.081678821329014 39.0 35.0 41.0 22.0 41.0 41 35.09859902864627 39.0 35.0 41.0 22.0 41.0 42 34.93279924167138 39.0 35.0 41.0 21.0 41.0 43 34.894471030114005 39.0 35.0 40.0 20.0 41.0 44 34.718236070553104 38.0 34.0 40.0 20.0 41.0 45 34.672025420316885 38.0 34.0 40.0 20.0 41.0 46 34.6605613058745 38.0 34.0 40.0 20.0 41.0 47 34.5029665530193 38.0 34.0 40.0 20.0 41.0 48 34.553989124507574 38.0 34.0 40.0 20.0 41.0 49 34.53947691633413 38.0 34.0 40.0 20.0 41.0 50 33.92659705128401 38.0 33.0 40.0 18.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 2.0 13 3.0 14 9.0 15 22.0 16 60.0 17 200.0 18 491.0 19 1354.0 20 2937.0 21 5913.0 22 10312.0 23 16051.0 24 23353.0 25 32460.0 26 40722.0 27 42525.0 28 39828.0 29 38555.0 30 42730.0 31 52031.0 32 66705.0 33 88052.0 34 128451.0 35 190932.0 36 190194.0 37 294738.0 38 339858.0 39 313720.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.32120415246075 21.525473827979678 26.315277161975526 18.838044857584052 2 35.33347602957889 26.063469251507225 26.93131723923535 11.671737479678526 3 25.86119732342614 26.17410980476096 35.8600761386396 12.104616733173309 4 22.77274094006248 25.257337389983743 38.694940908465455 13.274980761488322 5 23.378741317188272 29.337226902319323 34.03799797167479 13.246033808817609 6 18.323421040561406 40.36632164753008 31.399289576548888 9.91096773535962 7 80.36606683280587 2.6893145993548093 15.226912511912587 1.7177060559267356 8 40.687388200039756 51.96023871043365 6.113412937453178 1.2389601520734272 9 76.03411459527777 4.64078768327549 13.679116914091763 5.645980807354972 10 39.2219487210849 32.47312978733164 18.669051732485308 9.63586975909816 11 29.40908465454768 22.367789380341556 34.01215975863949 14.210966206471277 12 26.25641496068209 21.302103240733665 33.68156313544422 18.75991866314003 13 22.308417549599685 27.08354355548081 34.73399890939298 15.874039985526522 14 15.996554904928626 32.482812746851764 34.57351659608299 16.94711575213662 15 15.735013071995352 25.04854220496277 42.01502387613966 17.20142084690222 16 18.554741847202898 23.728754822368657 39.124456607600614 18.59204672282783 17 18.199377232814022 25.279710122769732 34.237517900734375 22.28339474368187 18 19.553615566121874 24.575147410317957 35.692204198327396 20.179032825232774 19 21.918092355048643 26.282915692000348 31.677547255390603 20.121444697560403 20 24.94967409196773 25.215904515826544 32.30607325413692 17.52834813806881 21 21.150896183385058 28.157281840373866 33.50788141941994 17.183940556821135 22 20.436293770799253 22.75052109611102 35.5919600858216 21.22122504726813 23 19.722761580055142 27.20595654899323 33.46074069544034 19.610541175511287 24 18.553060070023086 26.244795409257925 36.838870457290504 18.36327406342848 25 17.858944761264084 28.573088507346306 34.47622833437807 19.091738397011532 26 17.574571529041233 28.956992370847157 34.12697927337033 19.341456826741275 27 20.472120721023742 26.880812960896133 33.60267249682756 19.044393821252566 28 17.07849822394137 28.053521284673916 37.12202057883713 17.745959912547587 29 18.511015640527773 24.847850128171807 36.99374684666778 19.647387384632633 30 19.61538265527135 27.81017322304952 34.16239852003608 18.412045601643044 31 22.941326361602478 24.712645435503845 31.11838182457535 21.227646378318322 32 24.592882515123254 25.882194056701373 31.130307153668568 18.394616274506806 33 23.65287099749772 25.38341971552484 30.96299580574964 20.0007134812278 34 18.771283399839977 26.176708914947945 32.9365868077321 22.115420877479984 35 20.518344111996168 25.317167887229196 31.957384785522446 22.207103215252193 36 21.41962379154117 27.28846555669373 31.31290738504034 19.979003266724764 37 19.90790995866905 27.36720330647586 31.6120598712676 21.112826863587486 38 19.067683887045728 28.13623414415379 30.207572074344746 22.58850989445574 39 19.7361648345488 27.501337777302126 30.690445976730317 22.07205141141876 40 20.338393953756224 25.640986438760375 30.44618058209876 23.574439025384645 41 18.210945821293336 25.476478052807806 30.804246232564303 25.508329893334558 42 20.475229460659154 26.662640594024083 29.46071011767344 23.401419827643323 43 20.879008872648697 27.087671554013077 29.76903593397241 22.264283639365818 44 20.68407560862497 27.86139098261654 30.62128926057863 20.83324414817986 45 19.481502999169305 28.576757839374988 29.47487781633974 22.466861345115966 46 21.679585773184318 28.454803512366155 29.14433215608931 20.721278558360215 47 20.010447403692773 26.421534901972777 31.203337053628307 22.36468064070614 48 20.89939405058582 24.90655944063072 31.64605215547775 22.54799435330571 49 20.49984456301823 24.133043863806627 32.66638127417555 22.7007302989996 50 19.165226963474858 26.852681415342904 31.941178569062433 22.040913052119805 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 511.0 1 1133.0 2 1755.0 3 23793.0 4 45831.0 5 32067.5 6 18304.0 7 18145.5 8 17987.0 9 18267.5 10 18548.0 11 18241.5 12 17935.0 13 17403.5 14 16872.0 15 15856.5 16 14841.0 17 14239.5 18 13638.0 19 13409.0 20 13180.0 21 13519.5 22 13859.0 23 14947.5 24 16036.0 25 18433.0 26 20830.0 27 24373.0 28 27916.0 29 32810.0 30 37704.0 31 42803.5 32 47903.0 33 54517.5 34 61132.0 35 67289.5 36 73447.0 37 80325.5 38 87204.0 39 100269.5 40 113335.0 41 126037.0 42 138739.0 43 146559.5 44 154380.0 45 171099.5 46 187819.0 47 182872.0 48 177925.0 49 166723.0 50 155521.0 51 137933.5 52 120346.0 53 107164.0 54 93982.0 55 85769.0 56 77556.0 57 69283.5 58 61011.0 59 53091.5 60 45172.0 61 37984.5 62 30797.0 63 24510.0 64 18223.0 65 13838.0 66 9453.0 67 7262.5 68 5072.0 69 4265.5 70 3459.0 71 2740.5 72 2022.0 73 1558.5 74 1095.0 75 746.0 76 397.0 77 313.0 78 229.0 79 205.0 80 181.0 81 112.0 82 43.0 83 28.5 84 14.0 85 9.0 86 4.0 87 2.5 88 1.0 89 0.5 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1962210.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.1134124347282 #Duplication Level Percentage of deduplicated Percentage of total 1 74.29919135695842 26.831973410406317 2 7.298457951001253 5.2714444424405915 3 3.4872573217339777 3.7781028577741433 4 2.2740608796086565 3.284963937879528 5 1.7574660396708335 3.173404796533061 6 1.3828431007085105 2.996350993704289 7 1.159037072214984 2.9299748671227697 8 0.9920528686617287 2.8661131522429004 9 0.8584752850191032 2.7902224829624145 >10 6.4091468311020625 39.66838875320304 >50 0.060253265097895935 1.3930386441741907 >100 0.017008269851018065 1.1009881294222776 >500 0.002604706203716231 0.6756621363987529 >1k 0.0015321801198330768 1.098455878452874 >5k 3.0643602396661536E-4 0.6141880037692373 >10k+ 3.0643602396661536E-4 1.5267275135135852 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14100 0.7185775222835475 No Hit CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13492 0.6875920518191223 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCC 5928 0.3021083370281468 No Hit GAATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTT 5172 0.2635803507269864 No Hit GAATCTGACTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTT 4375 0.222962883687271 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATG 4227 0.21542036785053587 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTCTCCT 2070 0.10549329582460593 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0361836908383914 0.0 2 0.0 0.0 0.0 0.14590691108495013 0.0 3 0.0 0.0 0.0 0.253234872923897 0.0 4 0.0 0.0 0.0 0.43603895607503784 0.0 5 0.0 5.0962944842804796E-5 0.0 0.9178426366189144 0.0 6 0.0 5.0962944842804796E-5 0.0 1.3841026189857355 0.0 7 0.0 5.0962944842804796E-5 0.0 1.7223946468522737 0.0 8 0.0 5.0962944842804796E-5 0.0 2.3356317621457436 0.0 9 0.0 5.0962944842804796E-5 0.0 2.9622721319328718 0.0 10 0.0 5.0962944842804796E-5 0.0 3.9705739956477646 0.0 11 0.0 5.0962944842804796E-5 0.0 4.412830430993624 0.0 12 0.0 5.0962944842804796E-5 0.0 5.045178650603146 0.0 13 0.0 5.0962944842804796E-5 0.0 5.308147445992019 0.0 14 0.0 5.0962944842804796E-5 0.0 5.461291095244648 0.0 15 0.0 5.0962944842804796E-5 0.0 5.616575188180674 0.0 16 0.0 5.0962944842804796E-5 0.0 5.803609195753768 0.0 17 0.0 5.0962944842804796E-5 0.0 6.003944531930833 0.0 18 0.0 5.0962944842804796E-5 0.0 6.30477879533791 0.0 19 0.0 5.0962944842804796E-5 0.0 6.571213070976094 0.0 20 0.0 5.0962944842804796E-5 0.0 6.7592153745012 0.0 21 0.0 5.0962944842804796E-5 0.0 6.945688789681023 0.0 22 0.0 5.0962944842804796E-5 0.0 7.160446639248602 0.0 23 0.0 5.0962944842804796E-5 0.0 7.37698819188568 0.0 24 0.0 5.0962944842804796E-5 0.0 7.557193164849838 0.0 25 0.0 5.0962944842804796E-5 0.0 7.7166562192629735 0.0 26 0.0 5.0962944842804796E-5 0.0 7.870972016246987 0.0 27 5.0962944842804796E-5 5.0962944842804796E-5 0.0 8.050310619148817 0.0 28 5.0962944842804796E-5 5.0962944842804796E-5 0.0 8.218080633571331 0.0 29 5.0962944842804796E-5 5.0962944842804796E-5 0.0 8.397164421748947 0.0 30 5.0962944842804796E-5 5.0962944842804796E-5 0.0 8.628943894894023 0.0 31 5.0962944842804796E-5 5.0962944842804796E-5 0.0 8.825507973152721 0.0 32 5.0962944842804796E-5 5.0962944842804796E-5 0.0 9.031856936821237 0.0 33 5.0962944842804796E-5 5.0962944842804796E-5 0.0 9.228370052135093 0.0 34 5.0962944842804796E-5 5.0962944842804796E-5 0.0 9.429877536043543 0.0 35 5.0962944842804796E-5 5.0962944842804796E-5 0.0 9.643565163769424 0.0 36 5.0962944842804796E-5 5.0962944842804796E-5 0.0 9.844359166450074 0.0 37 5.0962944842804796E-5 5.0962944842804796E-5 0.0 10.057791979451741 0.0 38 5.0962944842804796E-5 5.0962944842804796E-5 0.0 10.290030119100402 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTTT 18405 0.0 43.246944 1 CGACGGT 175 0.0 42.74286 28 CGATGAA 3335 0.0 41.22939 19 GAGCGAT 1105 0.0 41.013577 7 CCGATGA 3290 0.0 40.990883 18 TGTACGA 140 0.0 40.857143 2 GTTTTTA 10320 0.0 40.01357 2 GTTTACG 155 0.0 39.741936 1 GCGCGAC 465 0.0 39.741932 9 CGAGGTA 150 0.0 39.600002 6 CACGACG 190 0.0 39.368423 26 CGCGCGA 45 2.3523171E-8 39.11111 2 TAATGCG 40 4.126523E-7 38.5 1 CCACCGA 3880 0.0 38.103092 15 GCTTGCG 110 0.0 38.0 1 ACGAGAT 585 0.0 37.982906 5 ACCGATG 3565 0.0 37.890602 17 TTAGCGA 215 0.0 37.860466 2 ACGTGCG 35 7.2928397E-6 37.714287 1 CGCACGA 35 7.2928397E-6 37.714287 2 >>END_MODULE