FastQCFastQC Report
Thu 26 May 2016
SRR1546478_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546478_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences954465
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95641.0020273137307287No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92720.9714342589827809No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA351.4457328E-744.02
AACGCGA207.855811E-444.02
GCGATCG302.5272639E-644.09
TCGTAAG700.044.01
TATACGA254.4422704E-544.02
TCTATCG207.855811E-444.016
CGTTTTT103050.043.231441
CGACGGT1800.041.55555728
GCGATAT750.041.0666669
ATTGCGA700.040.8571432
TATGCGA650.040.6153832
GTTTTTA57750.040.6095242
CGAGGAT1200.040.3333326
TTCGCGA603.6379788E-1240.3333322
GCGAGGT1600.039.8755
TCACGAC1950.039.4871825
CACGACG1950.039.4871826
ATTGCGG1400.039.2857132
CGCGAGT452.3501343E-839.111114
AGCGATA1350.039.111118
Produced by FastQC (version 0.11.5)