##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546478_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	954465
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70937121843127	31.0	31.0	34.0	30.0	34.0
2	32.07941516975478	33.0	31.0	34.0	30.0	34.0
3	32.11911803994908	33.0	31.0	34.0	30.0	34.0
4	35.822296260208596	37.0	35.0	37.0	35.0	37.0
5	35.788483600760635	37.0	35.0	37.0	35.0	37.0
6	35.835480609556136	37.0	35.0	37.0	35.0	37.0
7	36.152531522895025	37.0	35.0	37.0	35.0	37.0
8	18.487087530710923	30.0	0.0	37.0	0.0	37.0
9	28.014173385090075	30.0	17.0	39.0	17.0	39.0
10	33.86442981146506	33.0	32.0	39.0	27.0	39.0
11	35.98645209620049	37.0	35.0	39.0	32.0	39.0
12	36.77027339923413	38.0	35.0	39.0	33.0	39.0
13	36.9662868727507	39.0	37.0	39.0	33.0	39.0
14	38.21760357896832	40.0	37.0	41.0	33.0	41.0
15	38.431244728722376	40.0	37.0	41.0	34.0	41.0
16	38.49931637095126	40.0	38.0	41.0	34.0	41.0
17	38.4561539710728	40.0	37.0	41.0	34.0	41.0
18	38.37435945791621	40.0	37.0	41.0	34.0	41.0
19	38.32014479315637	40.0	37.0	41.0	34.0	41.0
20	38.14683932883867	40.0	36.0	41.0	34.0	41.0
21	38.07053165909698	40.0	36.0	41.0	34.0	41.0
22	38.06557705101811	40.0	36.0	41.0	34.0	41.0
23	37.87942669453568	39.0	36.0	41.0	34.0	41.0
24	37.63606418255253	39.0	35.0	41.0	33.0	41.0
25	37.53771065465994	39.0	35.0	41.0	33.0	41.0
26	37.48358190190316	39.0	35.0	41.0	33.0	41.0
27	37.340518510369684	39.0	35.0	41.0	33.0	41.0
28	37.30673937755706	39.0	35.0	41.0	33.0	41.0
29	37.196116148837305	39.0	35.0	41.0	33.0	41.0
30	37.06626120392052	39.0	35.0	41.0	33.0	41.0
31	36.94080558218479	39.0	35.0	41.0	32.0	41.0
32	36.566353926021385	39.0	35.0	41.0	31.0	41.0
33	36.26659542256657	39.0	35.0	41.0	30.0	41.0
34	35.96556081155412	39.0	35.0	41.0	27.0	41.0
35	35.69994080453448	39.0	35.0	41.0	25.0	41.0
36	35.52729225272797	39.0	35.0	41.0	24.0	41.0
37	35.43290115404965	39.0	35.0	41.0	24.0	41.0
38	35.34544587805734	39.0	35.0	41.0	23.0	41.0
39	35.27941097892537	39.0	35.0	41.0	23.0	41.0
40	35.21277783889404	39.0	35.0	41.0	23.0	41.0
41	35.183185344669525	39.0	35.0	41.0	23.0	41.0
42	35.04307544016805	39.0	35.0	40.0	22.0	41.0
43	35.03154227761102	39.0	35.0	40.0	22.0	41.0
44	34.81500945555887	38.0	35.0	40.0	21.0	41.0
45	34.75572179178912	38.0	34.0	40.0	20.0	41.0
46	34.76045847673828	38.0	34.0	40.0	20.0	41.0
47	34.66382004578481	38.0	34.0	40.0	20.0	41.0
48	34.64711749514126	38.0	34.0	40.0	20.0	41.0
49	34.618465842120976	38.0	34.0	40.0	20.0	41.0
50	34.03721561293499	38.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	2.0
14	6.0
15	13.0
16	27.0
17	87.0
18	183.0
19	507.0
20	1036.0
21	2021.0
22	3502.0
23	5641.0
24	8902.0
25	13955.0
26	18942.0
27	20905.0
28	19420.0
29	17840.0
30	19288.0
31	23508.0
32	30761.0
33	41052.0
34	62795.0
35	98963.0
36	95188.0
37	145745.0
38	168377.0
39	155798.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.47550198278617	22.21611059598833	26.632720948384698	20.675666472840806
2	36.97904061437559	23.306250098222563	27.78498949673377	11.929719790668072
3	26.019916916806796	23.40483936026989	38.3490227509652	12.226220971958114
4	23.134426092103954	25.174626623291584	38.29768509060049	13.393262194003972
5	21.524309429890042	29.369018245823575	36.164448146343766	12.942224177942618
6	18.66532560125306	37.97520076692179	33.230343700397604	10.129129931427554
7	81.11098887858643	2.855421623632087	14.312101543796787	1.721487953984693
8	41.95324082077394	50.23714855966431	6.727328922485372	1.0822816970763727
9	77.46318618283541	4.488692618377835	14.942087975986547	3.1060332228002077
10	37.85314286013631	32.82865270072763	19.90277275751337	9.415431681622689
11	29.31244204868696	22.005311876286715	34.84297486026203	13.839271214764292
12	27.66565562906969	21.044773773789505	34.83291686965997	16.45665372748084
13	20.679647760787457	28.34551293132802	35.40370783632716	15.571131471557365
14	16.37451347089731	31.696814445789002	35.33434960946708	16.594322473846603
15	15.51151692309304	24.40120905428696	44.064685452059535	16.02258857056047
16	17.778546096504325	23.13065434562818	41.767377536106615	17.32342202176088
17	18.216487770635904	22.71722902358913	34.79792344402362	24.26835976175135
18	20.03048828401251	23.694844755962766	37.34133781752081	18.933329142503915
19	22.911159654885196	25.505492605805347	32.74934125400093	18.834006485308525
20	24.504512999429	24.156359845567934	33.30892175197624	18.030205403026827
21	21.3669437852619	26.035946839328837	34.385860141545265	18.211249233863995
22	20.46801087520234	22.878366414693048	34.79415169754784	21.85947101255677
23	18.372491395703353	26.903553299492383	34.285804089201804	20.438151215602456
24	18.419847768121407	24.585815090129024	38.87643863316099	18.11789850858858
25	18.004641343579912	26.430094346047262	36.603542298565166	18.961722011807662
26	18.10899299607634	29.652527855919285	33.67048555997339	18.56799358803099
27	18.54714421167879	27.247201311729608	35.23335062050468	18.972303856086917
28	17.22284211574023	26.01865966798154	39.15313814545321	17.605360070825018
29	17.836589083937074	23.53360259412341	39.284101564750934	19.345706757188584
30	19.558286579392643	25.76878146396149	36.288601467838006	18.384330488807866
31	23.16627639567716	24.94779798106793	32.76840952785068	19.117516095404234
32	23.67609079431933	26.13317408181547	32.78873505052569	17.402000073339515
33	23.290115404965086	25.5865851550345	32.10856343606104	19.01473600393938
34	19.60501432739807	26.23647802695751	33.81873615061841	20.33977149502601
35	20.747643968086834	26.008077823702287	33.36455501249391	19.879723195716974
36	24.149654518499894	25.991943130444806	31.71462547081349	18.14377688024181
37	21.38129737601693	28.385535352265407	31.97110423116615	18.262063040551514
38	20.963785995295794	29.01866490651831	30.437889288763863	19.579659809422033
39	21.006951538296324	28.72792611567737	30.6427160765455	19.62240626948081
40	22.357970171771623	26.719471117327508	30.464501055565158	20.45805765533571
41	19.71261387269308	25.71000508138067	31.299942899949183	23.277438145977065
42	21.72798373958186	27.252963702178707	28.895454521643018	22.123598036596416
43	21.555321567579742	27.08396850591693	29.91015909436176	21.450550832141566
44	20.60431760200741	28.611525828605554	31.08589628744899	19.698260281938047
45	19.451839512187455	29.746821517813643	29.428004169875273	21.37333480012363
46	21.488373067634747	29.256494475962974	29.79365403655451	19.46147841984777
47	20.96451939044386	26.92220249040038	30.830884317392464	21.28239380176329
48	21.201615564740457	25.13837594882997	31.99027727575134	21.669731210678233
49	20.509709627906734	24.64595349227054	33.026774161441224	21.817562718381502
50	19.57442127265012	27.475706285720275	32.22307784989496	20.726794591734638
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	339.0
1	710.0
2	1081.0
3	14919.0
4	28757.0
5	19445.0
6	10133.0
7	10183.5
8	10234.0
9	10317.5
10	10401.0
11	10314.0
12	10227.0
13	9812.0
14	9397.0
15	8990.5
16	8584.0
17	8092.5
18	7601.0
19	7309.0
20	7017.0
21	7149.5
22	7282.0
23	7650.0
24	8018.0
25	8970.0
26	9922.0
27	11794.0
28	13666.0
29	15952.5
30	18239.0
31	21034.0
32	23829.0
33	26666.0
34	29503.0
35	32457.5
36	35412.0
37	38447.0
38	41482.0
39	47924.0
40	54366.0
41	61237.0
42	68108.0
43	72781.0
44	77454.0
45	80132.5
46	82811.0
47	81688.5
48	80566.0
49	76048.0
50	71530.0
51	63778.0
52	56026.0
53	49822.5
54	43619.0
55	39048.5
56	34478.0
57	31352.5
58	28227.0
59	25359.5
60	22492.0
61	19509.0
62	16526.0
63	13668.5
64	10811.0
65	8510.5
66	6210.0
67	5080.5
68	3951.0
69	3246.0
70	2541.0
71	2147.0
72	1753.0
73	1364.0
74	975.0
75	731.0
76	487.0
77	363.0
78	239.0
79	159.5
80	80.0
81	64.0
82	48.0
83	36.5
84	25.0
85	19.0
86	13.0
87	8.0
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	954465.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.13317922115847
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.0674168883441	28.40962437210725
2	9.241742447086487	7.602844967835923
3	5.197779354613655	6.414035692360827
4	3.434309974084561	5.650563506601294
5	2.671482925813772	5.494329298688135
6	2.128109311564739	5.2521541028885155
7	1.635178779657736	4.70820712616078
8	1.370897486583445	4.511149760757803
9	1.0156605781258747	3.7599613729125325
>10	4.199542940915787	23.951356737521827
>50	0.021499773520557458	0.598615209685015
>100	0.014332097238840944	1.2454762488355144
>500	0.0015355818378058846	0.41877309631358417
>1k	0.0	0.0
>5k	5.118606126019616E-4	1.9829085073309882
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9564	1.0020273137307287	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9272	0.9714342589827809	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0412796697626419	0.0
2	0.0	0.0	0.0	0.1613469325747932	0.0
3	0.0	0.0	0.0	0.2796330928844955	0.0
4	0.0	0.0	0.0	0.4013766874636577	0.0
5	0.0	0.0	0.0	0.6861435463846238	0.0
6	0.0	0.0	0.0	1.0719093942679931	0.0
7	0.0	0.0	0.0	1.3244068666740005	0.0
8	0.0	0.0	0.0	1.8163054695562435	0.0
9	0.0	0.0	0.0	2.3189954581886187	0.0
10	1.0477073543817741E-4	0.0	0.0	2.9293897628514403	0.0
11	1.0477073543817741E-4	0.0	0.0	3.2903249464359616	0.0
12	1.0477073543817741E-4	0.0	0.0	3.669699779457602	0.0
13	1.0477073543817741E-4	0.0	0.0	3.8644685766371736	0.0
14	1.0477073543817741E-4	0.0	0.0	3.969553624281666	0.0
15	1.0477073543817741E-4	0.0	0.0	4.0750577548679106	0.0
16	1.0477073543817741E-4	0.0	0.0	4.232423399496053	0.0
17	1.0477073543817741E-4	0.0	0.0	4.406028508117113	0.0
18	1.0477073543817741E-4	0.0	0.0	4.6660694734746695	0.0
19	1.0477073543817741E-4	0.0	0.0	4.831816776937866	0.0
20	1.0477073543817741E-4	0.0	0.0	4.99368756318985	0.0
21	1.0477073543817741E-4	0.0	0.0	5.140995217215927	0.0
22	1.0477073543817741E-4	0.0	0.0	5.304437564499484	0.0
23	1.0477073543817741E-4	0.0	0.0	5.486633873426475	0.0
24	1.0477073543817741E-4	0.0	0.0	5.63980868863709	0.0
25	1.0477073543817741E-4	0.0	0.0	5.769829171315868	0.0
26	1.0477073543817741E-4	0.0	0.0	5.893249097662041	0.0
27	1.0477073543817741E-4	0.0	0.0	6.0487288690522965	0.0
28	1.0477073543817741E-4	0.0	0.0	6.191426610719094	0.0
29	1.0477073543817741E-4	0.0	0.0	6.338524723274295	0.0
30	1.0477073543817741E-4	0.0	0.0	6.536436642517012	0.0
31	1.0477073543817741E-4	0.0	0.0	6.702498258186523	0.0
32	1.0477073543817741E-4	0.0	0.0	6.861435463846238	0.0
33	1.0477073543817741E-4	0.0	0.0	7.028125703928379	0.0
34	1.0477073543817741E-4	0.0	0.0	7.196387505042091	0.0
35	1.0477073543817741E-4	0.0	0.0	7.382355560444856	0.0
36	1.0477073543817741E-4	0.0	0.0	7.540768912427381	0.0
37	1.0477073543817741E-4	0.0	0.0	7.714164479577565	0.0
38	1.0477073543817741E-4	0.0	0.0	7.88630279790249	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGA	35	1.4457328E-7	44.0	2
AACGCGA	20	7.855811E-4	44.0	2
GCGATCG	30	2.5272639E-6	44.0	9
TCGTAAG	70	0.0	44.0	1
TATACGA	25	4.4422704E-5	44.0	2
TCTATCG	20	7.855811E-4	44.0	16
CGTTTTT	10305	0.0	43.23144	1
CGACGGT	180	0.0	41.555557	28
GCGATAT	75	0.0	41.066666	9
ATTGCGA	70	0.0	40.857143	2
TATGCGA	65	0.0	40.615383	2
GTTTTTA	5775	0.0	40.609524	2
CGAGGAT	120	0.0	40.333332	6
TTCGCGA	60	3.6379788E-12	40.333332	2
GCGAGGT	160	0.0	39.875	5
TCACGAC	195	0.0	39.48718	25
CACGACG	195	0.0	39.48718	26
ATTGCGG	140	0.0	39.285713	2
CGCGAGT	45	2.3501343E-8	39.11111	4
AGCGATA	135	0.0	39.11111	8
>>END_MODULE