FastQCFastQC Report
Thu 26 May 2016
SRR1546477_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546477_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1046374
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67470.644798131452043No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62640.5986387276442265No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCC14130.13503775896572354No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA207.85616E-444.032
TAGCGCG254.4425666E-544.01
TAAGTCG254.4425666E-544.01
TCGATAG302.5274985E-644.01
TCTAGCG408.310963E-944.01
CGCGTAA351.4459147E-744.031
GCGTAAC351.4459147E-744.032
TAGTACG302.5274985E-644.01
CGTTTTT92650.043.5250931
GAGCGAT5300.042.3396227
CGTAAGA1100.042.02
GCGTAAG800.041.251
GAGACCG2350.041.191497
CGACGGT1500.041.06666628
GTTTTTA52500.040.482
CGTATGG557.8216544E-1140.02
GCTACGG557.8216544E-1140.02
CGTTGAG1550.039.7419363
CGGTCTA1550.039.74193631
TGCGCGA501.3478711E-939.62
Produced by FastQC (version 0.11.5)