##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546477_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1046374 Sequences flagged as poor quality 0 Sequence length 50 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.51476049672488 31.0 31.0 34.0 30.0 34.0 2 31.887206677535946 31.0 31.0 34.0 30.0 34.0 3 31.94884716172229 31.0 31.0 34.0 30.0 34.0 4 35.66611937987756 37.0 35.0 37.0 33.0 37.0 5 35.611118968934626 37.0 35.0 37.0 33.0 37.0 6 35.662140878882695 37.0 35.0 37.0 33.0 37.0 7 36.030466162194394 37.0 35.0 37.0 35.0 37.0 8 18.313504540441564 28.0 0.0 37.0 0.0 37.0 9 27.850904170019515 27.0 17.0 39.0 17.0 39.0 10 33.561826842027806 32.0 32.0 39.0 27.0 39.0 11 35.75487923056192 37.0 35.0 39.0 32.0 39.0 12 36.49564209355355 37.0 35.0 39.0 32.0 39.0 13 36.67549365714362 39.0 35.0 39.0 33.0 39.0 14 37.864325757329595 40.0 36.0 41.0 33.0 41.0 15 38.093380569471336 40.0 37.0 41.0 33.0 41.0 16 38.16184748474255 40.0 37.0 41.0 33.0 41.0 17 38.102813143292934 40.0 37.0 41.0 33.0 41.0 18 38.050546936372655 40.0 37.0 41.0 33.0 41.0 19 38.025789058214365 40.0 37.0 41.0 34.0 41.0 20 37.851885654651205 40.0 36.0 41.0 33.0 41.0 21 37.74556802825758 40.0 36.0 41.0 33.0 41.0 22 37.740346185971745 40.0 36.0 41.0 33.0 41.0 23 37.54175658034317 39.0 35.0 41.0 33.0 41.0 24 37.36490394447874 39.0 35.0 41.0 33.0 41.0 25 37.252003585716004 39.0 35.0 41.0 32.0 41.0 26 37.18688059909746 39.0 35.0 41.0 32.0 41.0 27 37.01755395298431 39.0 35.0 41.0 32.0 41.0 28 36.98626494924377 39.0 35.0 41.0 32.0 41.0 29 36.861120402456486 39.0 35.0 40.0 31.0 41.0 30 36.75677339077615 39.0 35.0 40.0 31.0 41.0 31 36.665176122495396 39.0 35.0 41.0 31.0 41.0 32 36.30326632733612 39.0 35.0 41.0 30.0 41.0 33 36.02959266954263 39.0 35.0 41.0 29.0 41.0 34 35.76125744714605 39.0 35.0 41.0 26.0 41.0 35 35.48219661421251 39.0 35.0 41.0 24.0 41.0 36 35.326411015564226 39.0 35.0 41.0 24.0 41.0 37 35.235347017414426 39.0 35.0 41.0 23.0 41.0 38 35.16652554440382 39.0 35.0 41.0 23.0 41.0 39 35.099267565899 39.0 35.0 40.0 23.0 41.0 40 35.037952013333665 39.0 35.0 40.0 23.0 41.0 41 35.04177569396793 38.0 35.0 40.0 23.0 41.0 42 34.87673814525208 38.0 34.0 40.0 22.0 41.0 43 34.88726019568529 38.0 34.0 40.0 22.0 41.0 44 34.67888250281448 38.0 34.0 40.0 21.0 41.0 45 34.595143801355924 38.0 34.0 40.0 21.0 41.0 46 34.59438116772779 38.0 34.0 40.0 22.0 41.0 47 34.44691859698349 38.0 34.0 40.0 20.0 41.0 48 34.444485432550884 38.0 34.0 40.0 20.0 41.0 49 34.385770288634845 38.0 34.0 40.0 20.0 41.0 50 33.76280373938955 37.0 33.0 40.0 19.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 1.0 11 0.0 12 2.0 13 3.0 14 6.0 15 18.0 16 39.0 17 137.0 18 364.0 19 877.0 20 1705.0 21 3075.0 22 4760.0 23 7349.0 24 10810.0 25 15878.0 26 20923.0 27 22536.0 28 21648.0 29 21974.0 30 24396.0 31 29881.0 32 38112.0 33 50006.0 34 73342.0 35 108513.0 36 104145.0 37 157824.0 38 173736.0 39 154311.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.40351920059176 22.501323618514988 27.19352736210953 17.901629818783725 2 35.65187972942753 24.71831295502373 27.467234468746355 12.162572846802385 3 26.47189245910162 24.922255331267788 36.07916481105226 12.526687398578328 4 24.063289034322334 25.71011894408691 36.719757945055974 13.506834076534776 5 22.32175111384648 30.02903359601825 34.19475254545698 13.454462744678288 6 18.81392312882392 39.66488081699278 31.338221324306602 10.182974729876697 7 81.88869371754267 3.0318031602467186 13.280337623067851 1.799165499142754 8 41.85807369066892 50.866707314975336 6.019644983533612 1.2555740108221343 9 77.709786367016 4.668216144514294 13.585008801824205 4.036988686645501 10 38.46234711489391 31.05180365720096 19.298262380372602 11.187586847532527 11 32.170906387199985 22.362367566472408 31.135139061176982 14.331586985150624 12 28.86730748279296 21.425991089228134 32.32142618222548 17.38527524575343 13 21.77127872061041 29.120849715302562 33.1866044072196 15.92126715686743 14 16.88421157253525 32.54897388505448 33.90317419966475 16.66364034274552 15 15.790052122854734 24.57132918057979 43.503087806080806 16.135530890484663 16 17.759520018654896 22.568794713935937 41.21958305538938 18.452102212019796 17 18.027397469738354 23.33716242949462 33.875555011879115 24.759885088887913 18 20.18437002448455 24.105434576929472 35.72642286601158 19.983772532574395 19 23.35761400799332 26.44121509135357 31.12911826937596 19.072052631277153 20 25.34007916863378 24.61414370005371 31.369185396426136 18.67659173488638 21 21.32478444609671 27.75154963712783 32.65515007062484 18.268515846150613 22 20.977489884114092 23.542251623224583 32.836347233398385 22.643911259262943 23 19.08810807607987 27.72909112802879 32.331556403351 20.851244392540337 24 18.87718922679654 25.42589934382926 37.25102114540308 18.445890283971124 25 18.32643012918899 27.17995668852628 34.992650811277805 19.500962371006924 26 18.02433928977593 30.22466154548947 32.51695856357287 19.234040601161727 27 19.696494752354322 28.05211138655968 32.937745012777455 19.31364884830854 28 18.208499064388068 26.54634002756185 37.432218308176616 17.81294259987347 29 18.728294089876087 24.060421990607566 36.774709616255755 20.436574303260592 30 20.44335964005222 26.892296635810904 33.25894947695566 19.405394247181217 31 24.836052883577 24.30488525135372 29.58511966084784 21.273942204221434 32 26.002079562374448 26.028551932674166 29.580150118408906 18.38921838654248 33 24.75740031766844 25.991567068753618 28.997853539938873 20.253179073639064 34 21.22730495979449 26.737189570841785 30.48690047726721 21.548604992096514 35 21.08509959154184 26.995510209542665 30.85588900335826 21.06350119555723 36 25.52787053195129 27.021026898604134 28.661644880320036 18.78945768912454 37 20.47375030342879 30.93549725050508 29.31389732543049 19.27685512063564 38 20.958567395596603 30.135400917836265 28.4403091055397 20.465722581027435 39 20.871982675410514 30.01164019748197 28.80776854164954 20.30860858545797 40 21.79603086468127 26.762801828027072 29.808940206847645 21.63222710044401 41 19.01356493949582 26.51843413540474 29.59496317760189 24.87303774749755 42 21.449500847689258 27.122998086726163 28.031181967441853 23.39631909814273 43 20.87074028980078 27.490648659083654 28.966602763447867 22.672008287667698 44 21.04792359137364 28.583183450659135 29.708498108706827 20.6603948492604 45 19.42766162003261 30.514232960681365 28.11623759764673 21.9418678216393 46 22.374026877579144 29.039807946298357 28.51647690022879 20.069688275893704 47 20.94327649578449 26.79758862509963 29.758097964972368 22.50103691414351 48 21.478171284836968 25.380217780640574 30.794247563490686 22.347363371031772 49 20.878576875954487 24.85134378338911 31.826670005179793 22.44340933547661 50 19.81174990968812 27.92424123688089 30.330742162936 21.933266690494985 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 303.0 1 623.0 2 943.0 3 11106.0 4 21269.0 5 15203.5 6 9138.0 7 9205.0 8 9272.0 9 9482.0 10 9692.0 11 9772.0 12 9852.0 13 9476.5 14 9101.0 15 8684.5 16 8268.0 17 7819.0 18 7370.0 19 7246.0 20 7122.0 21 7167.0 22 7212.0 23 7609.5 24 8007.0 25 9247.5 26 10488.0 27 11891.0 28 13294.0 29 15877.0 30 18460.0 31 21521.0 32 24582.0 33 27500.5 34 30419.0 35 33695.5 36 36972.0 37 40973.5 38 44975.0 39 52280.5 40 59586.0 41 68083.5 42 76581.0 43 81497.0 44 86413.0 45 90447.0 46 94481.0 47 93603.0 48 92725.0 49 87148.5 50 81572.0 51 72903.0 52 64234.0 53 57572.0 54 50910.0 55 45458.5 56 40007.0 57 36739.0 58 33471.0 59 30151.0 60 26831.0 61 23200.0 62 19569.0 63 16001.0 64 12433.0 65 9819.5 66 7206.0 67 5960.5 68 4715.0 69 4162.5 70 3610.0 71 3104.5 72 2599.0 73 2055.5 74 1512.0 75 1094.5 76 677.0 77 480.0 78 283.0 79 217.5 80 152.0 81 97.5 82 43.0 83 28.5 84 14.0 85 11.0 86 8.0 87 5.0 88 2.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1046374.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.21389839862092 #Duplication Level Percentage of deduplicated Percentage of total 1 72.02451216237053 29.684109264701753 2 8.281994698889715 6.826665761159155 3 4.279030003260242 5.290665233970544 4 2.922525446203029 4.817946672287835 5 2.267755487348619 4.673152212425053 6 1.7977945703434632 4.44564736582367 7 1.4874808822742371 4.291342016635899 8 1.2146634502310805 4.004881282107367 9 0.9852271802646057 3.6544547616286707 >10 4.699269040140046 28.691238523733126 >50 0.02440768026367587 0.69713804425803 >100 0.013480077453957118 1.1483943370151204 >500 6.972453588340387E-4 0.19645981641354934 >1k 6.972453588340387E-4 0.3316157408209205 >5k 4.6483023922269244E-4 1.2462889670193518 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6747 0.644798131452043 No Hit CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6264 0.5986387276442265 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCC 1413 0.13503775896572354 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.03641145517759425 0.0 2 0.0 0.0 0.0 0.12873026279322689 0.0 3 0.0 0.0 0.0 0.21885100356086828 0.0 4 0.0 0.0 0.0 0.35130842318329775 0.0 5 0.0 0.0 0.0 0.6784381110386917 0.0 6 0.0 0.0 0.0 1.1037162620630865 0.0 7 0.0 0.0 0.0 1.4086741451909164 0.0 8 0.0 0.0 0.0 2.0348364924969466 0.0 9 0.0 0.0 0.0 2.6616678166697567 0.0 10 0.0 0.0 0.0 3.5222587717202454 0.0 11 0.0 0.0 0.0 3.9649303212809186 0.0 12 0.0 0.0 0.0 4.4635092232796305 0.0 13 0.0 0.0 0.0 4.6945929466901895 0.0 14 9.556812382570668E-5 0.0 0.0 4.8106126490145975 0.0 15 9.556812382570668E-5 0.0 0.0 4.9326531431400245 0.0 16 9.556812382570668E-5 0.0 0.0 5.126465298258558 0.0 17 9.556812382570668E-5 0.0 0.0 5.343596075590563 0.0 18 9.556812382570668E-5 0.0 0.0 5.671490308436563 0.0 19 9.556812382570668E-5 0.0 0.0 5.863008828583279 0.0 20 9.556812382570668E-5 0.0 0.0 6.0454483769665535 0.0 21 9.556812382570668E-5 0.0 0.0 6.23983394082804 0.0 22 9.556812382570668E-5 0.0 0.0 6.465852553675837 0.0 23 9.556812382570668E-5 0.0 0.0 6.699421048305864 0.0 24 9.556812382570668E-5 0.0 0.0 6.893806612167351 0.0 25 9.556812382570668E-5 0.0 0.0 7.061146396986164 0.0 26 9.556812382570668E-5 0.0 0.0 7.216731302574414 0.0 27 1.9113624765141335E-4 0.0 0.0 7.392481082289889 0.0 28 1.9113624765141335E-4 0.0 0.0 7.572818131948997 0.0 29 1.9113624765141335E-4 0.0 0.0 7.760896199637988 0.0 30 1.9113624765141335E-4 0.0 0.0 7.995133671134795 0.0 31 1.9113624765141335E-4 0.0 0.0 8.20136968235067 0.0 32 1.9113624765141335E-4 0.0 0.0 8.407605693566545 0.0 33 1.9113624765141335E-4 0.0 0.0 8.609923411705566 0.0 34 1.9113624765141335E-4 0.0 0.0 8.804595679938531 0.0 35 1.9113624765141335E-4 0.0 0.0 9.028702930309812 0.0 36 1.9113624765141335E-4 0.0 0.0 9.23264530655387 0.0 37 1.9113624765141335E-4 0.0 0.0 9.457325965668106 0.0 38 1.9113624765141335E-4 0.0 0.0 9.686402758478327 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCATA 20 7.85616E-4 44.0 32 TAGCGCG 25 4.4425666E-5 44.0 1 TAAGTCG 25 4.4425666E-5 44.0 1 TCGATAG 30 2.5274985E-6 44.0 1 TCTAGCG 40 8.310963E-9 44.0 1 CGCGTAA 35 1.4459147E-7 44.0 31 GCGTAAC 35 1.4459147E-7 44.0 32 TAGTACG 30 2.5274985E-6 44.0 1 CGTTTTT 9265 0.0 43.525093 1 GAGCGAT 530 0.0 42.339622 7 CGTAAGA 110 0.0 42.0 2 GCGTAAG 80 0.0 41.25 1 GAGACCG 235 0.0 41.19149 7 CGACGGT 150 0.0 41.066666 28 GTTTTTA 5250 0.0 40.48 2 CGTATGG 55 7.8216544E-11 40.0 2 GCTACGG 55 7.8216544E-11 40.0 2 CGTTGAG 155 0.0 39.741936 3 CGGTCTA 155 0.0 39.741936 31 TGCGCGA 50 1.3478711E-9 39.6 2 >>END_MODULE