FastQCFastQC Report
Thu 26 May 2016
SRR1546476_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546476_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1280281
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106770.8339575452576427No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98170.7667847917761804No Hit
CGGTCGGAGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC13470.10521127783666243No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAA302.5279442E-644.020
TCGTAGA302.5279442E-644.02
TACTCGC207.8568177E-444.025
TACGTAG502.7284841E-1144.01
CGTTTTT119700.043.099421
CGGTCTA1900.042.84210631
GTTTTTA64450.040.791312
AGCGATA2100.039.8095258
GAGCGAT8100.039.1111157
TGCGTTG452.3512257E-839.111111
CGACGGT2150.038.8837228
CTATCTC7050.038.6950386
TACGGGA1600.038.54
GCGTAAG750.038.133331
TCACGAC2200.038.00000425
GCGCGAC9250.037.8162169
GCGATAT1400.037.7142879
AGCGCGA4750.037.515798
TTATTTT71000.037.461976
CGTAAGA1000.037.42
Produced by FastQC (version 0.11.5)