##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546476_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1280281
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.572907822579573	31.0	31.0	34.0	30.0	34.0
2	31.944533270430476	31.0	31.0	34.0	30.0	34.0
3	31.998958822321036	33.0	31.0	34.0	30.0	34.0
4	35.709539546396456	37.0	35.0	37.0	35.0	37.0
5	35.672585940117834	37.0	35.0	37.0	33.0	37.0
6	35.71390577537275	37.0	35.0	37.0	33.0	37.0
7	36.063651651473386	37.0	35.0	37.0	35.0	37.0
8	18.463904408485323	30.0	0.0	37.0	0.0	37.0
9	27.942136921503952	30.0	17.0	39.0	17.0	39.0
10	33.71856881419001	32.0	32.0	39.0	27.0	39.0
11	35.886273404041766	37.0	35.0	39.0	32.0	39.0
12	36.60776032761558	38.0	35.0	39.0	33.0	39.0
13	36.81210218694177	39.0	35.0	39.0	33.0	39.0
14	37.96319557972039	40.0	37.0	41.0	33.0	41.0
15	38.16639081576623	40.0	37.0	41.0	33.0	41.0
16	38.21007028925681	40.0	37.0	41.0	33.0	41.0
17	38.1307283322958	40.0	37.0	41.0	33.0	41.0
18	38.05143324004652	40.0	37.0	41.0	33.0	41.0
19	38.0099048568244	40.0	37.0	41.0	33.0	41.0
20	37.88914074332119	40.0	36.0	41.0	33.0	41.0
21	37.77522590743751	40.0	36.0	41.0	33.0	41.0
22	37.77467837138878	40.0	36.0	41.0	33.0	41.0
23	37.62853076785487	39.0	35.0	41.0	33.0	41.0
24	37.42431544325035	39.0	35.0	41.0	33.0	41.0
25	37.28579897694334	39.0	35.0	41.0	33.0	41.0
26	37.240062142607755	39.0	35.0	41.0	33.0	41.0
27	37.11799987658959	39.0	35.0	41.0	32.0	41.0
28	37.06974328291992	39.0	35.0	41.0	32.0	41.0
29	36.917147876130315	39.0	35.0	40.0	32.0	41.0
30	36.75207317768521	39.0	35.0	40.0	31.0	41.0
31	36.672269603313644	39.0	35.0	41.0	31.0	41.0
32	36.359189896593016	39.0	35.0	41.0	30.0	41.0
33	36.129234129070106	39.0	35.0	41.0	30.0	41.0
34	35.84098178446763	39.0	35.0	41.0	27.0	41.0
35	35.57093169390157	39.0	35.0	41.0	25.0	41.0
36	35.44513821575107	39.0	35.0	41.0	25.0	41.0
37	35.4102404081604	39.0	35.0	41.0	24.0	41.0
38	35.31366473453875	39.0	35.0	41.0	24.0	41.0
39	35.23670194277663	38.0	35.0	40.0	24.0	41.0
40	35.16113806265968	38.0	35.0	40.0	23.0	41.0
41	35.12040013090876	38.0	35.0	40.0	23.0	41.0
42	34.962380914814794	38.0	34.0	40.0	23.0	41.0
43	34.967552435754335	38.0	34.0	40.0	23.0	41.0
44	34.71656300452791	38.0	34.0	40.0	23.0	41.0
45	34.622309477372546	38.0	34.0	40.0	23.0	41.0
46	34.61236790985729	38.0	34.0	40.0	23.0	41.0
47	34.50150787209995	38.0	34.0	40.0	22.0	41.0
48	34.46607580679554	38.0	34.0	40.0	22.0	41.0
49	34.43423670272386	37.0	34.0	40.0	22.0	41.0
50	33.873109887594985	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	5.0
14	12.0
15	21.0
16	62.0
17	128.0
18	375.0
19	808.0
20	1610.0
21	2896.0
22	4586.0
23	7121.0
24	11341.0
25	17540.0
26	23731.0
27	27324.0
28	27041.0
29	27391.0
30	30428.0
31	37076.0
32	47361.0
33	62892.0
34	93527.0
35	132990.0
36	132812.0
37	196065.0
38	211354.0
39	183781.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.363583463317816	22.221293606637918	26.65617938561925	19.758943544425012
2	37.38429297943186	22.96472415040136	27.075462339908192	12.575520530258592
3	27.685797102354876	23.42766939445325	35.79636033027124	13.090173172920633
4	24.804398409411686	25.47589162066765	35.35825338343692	14.361456586483747
5	21.73944626218775	29.77112055868985	34.136021701485845	14.353411477636548
6	19.381604507135545	38.37930891733924	31.417009234691445	10.82207734083377
7	81.60880306745159	3.173678278440436	13.022375556616087	2.195143097491879
8	41.944151323029864	50.077678259694544	6.38406724773702	1.5941031695385621
9	77.11947611500912	4.643277530479637	14.6901344314256	3.547111923085635
10	38.9544170381346	29.289976184915655	19.380198565783605	12.375408211166143
11	32.21628689326796	21.519182117050867	30.68529486886082	15.579236120820351
12	29.897655280364233	19.882822599101292	32.50778540023636	17.71173672029812
13	23.77899851673187	23.66199295310951	34.1132142084433	18.44579432171531
14	18.4104895722111	27.69485761328958	34.921396162248755	18.973256652250562
15	17.96324400658918	22.685879115600404	41.19509701385868	18.155779863951743
16	21.808884143402892	21.068499805901986	39.01088901577076	18.111727034924364
17	21.15355925769421	21.473489023112897	33.9160699877605	23.456881731432397
18	22.54192634273257	23.262471285600583	34.95912225519241	19.236480116474432
19	24.50672938206534	26.320862373182134	30.776212409619454	18.39619583513307
20	26.14785347904093	25.030833074926516	29.849618950839695	18.971694495192853
21	23.742600257287265	26.643994560569123	31.81121956820417	17.80218561393944
22	21.858873169249563	22.465302539051972	33.04797931079192	22.627844980906538
23	20.85651509317095	25.65905453568396	32.32329465172099	21.161135719424095
24	21.828957861594446	23.840391289099816	35.03504308819704	19.295607761108695
25	22.507402671757216	23.742756472993037	32.552931739204126	21.196909116045617
26	19.281470239736436	26.70241923452742	31.947673987194996	22.068436538541146
27	19.84275327057107	24.119939294576735	34.000035929612324	22.037271505239868
28	19.51407542562922	25.033723065483283	35.5833602154527	19.868841293434798
29	20.940090495758355	24.312709475497957	34.51617262147919	20.2310274072645
30	22.506621593228363	23.531240407379318	34.48188327406249	19.48025472532983
31	26.414591796644643	22.01485455146175	30.98796279879183	20.582590853101777
32	26.38678540101743	23.22638545756752	31.16104980078592	19.225779340629128
33	25.356464713605842	23.26067480498422	31.0673984851763	20.315461996233637
34	21.74155517421566	24.12142334378156	32.762026461378404	21.37499502062438
35	20.685927542469194	24.368478482458148	32.97229280134595	21.973301173726707
36	23.910063493873608	25.967033799611176	29.011912228643556	21.110990477871656
37	21.723199828787585	27.11225113861723	29.294115901118584	21.870433131476606
38	21.607600206517162	28.01220981956305	27.3282974596983	23.051892514221485
39	21.79240338644407	25.695530902981456	28.157959073047245	24.35410663752723
40	22.544425794024907	23.383538457572985	30.3553672982728	23.71666845012931
41	20.08598112445627	23.623173350225457	30.516035151658112	25.774810373660156
42	21.176835397854067	26.276028465625906	27.782963271344336	24.764172865175695
43	20.745211402809225	24.942493093313107	29.334653876766115	24.97764162711155
44	21.847547530581178	25.202826566980217	30.28186780870762	22.667758093730985
45	20.96570987150477	26.53198789953143	29.301458039289813	23.200844189673987
46	22.93887045109628	26.267827141072935	29.118295124273498	21.67500728355728
47	21.526368039516324	25.311630806049614	29.671376830555168	23.490624323878897
48	21.263457006703998	24.037769833341276	31.145115798797296	23.553657361157434
49	22.510292662313976	22.841235634989506	31.197682383789182	23.450789318907333
50	20.454884513634116	24.187815018734167	31.889717960354012	23.467582507277697
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	421.0
1	1009.5
2	1598.0
3	17530.0
4	33462.0
5	22988.0
6	12514.0
7	12205.5
8	11897.0
9	12040.0
10	12183.0
11	12050.5
12	11918.0
13	11180.0
14	10442.0
15	9944.5
16	9447.0
17	8819.5
18	8192.0
19	7733.0
20	7274.0
21	7334.5
22	7395.0
23	7955.5
24	8516.0
25	9581.0
26	10646.0
27	12127.5
28	13609.0
29	15776.0
30	17943.0
31	20415.5
32	22888.0
33	25766.0
34	28644.0
35	31774.5
36	34905.0
37	39297.0
38	43689.0
39	50220.0
40	56751.0
41	63772.0
42	70793.0
43	77180.0
44	83567.0
45	91169.5
46	98772.0
47	104449.0
48	110126.0
49	105412.5
50	100699.0
51	89380.0
52	78061.0
53	71175.5
54	64290.0
55	62369.5
56	60449.0
57	59768.5
58	59088.0
59	56758.5
60	54429.0
61	49813.0
62	45197.0
63	38305.0
64	31413.0
65	25783.5
66	20154.0
67	16965.5
68	13777.0
69	12137.0
70	10497.0
71	8690.5
72	6884.0
73	5465.0
74	4046.0
75	3020.0
76	1994.0
77	1448.5
78	903.0
79	685.5
80	468.0
81	338.5
82	209.0
83	145.5
84	82.0
85	57.5
86	33.0
87	21.5
88	10.0
89	7.5
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1280281.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.28812180704505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.0798470505522	29.21256986485793
2	9.094525865930382	7.691808351915717
3	5.2769094152927325	6.694517643559259
4	3.716208239400566	6.286058667524623
5	2.8743403643746728	6.077522772179121
6	2.24451286782021	5.694974013111664
7	1.8118290550060712	5.363319344014805
8	1.3711222185137957	4.638574671108578
9	1.047111282946101	3.9852332530880346
>10	3.4355869169077735	19.72996960518921
>50	0.02721002454274031	0.7583962158810251
>100	0.01911048204007274	1.7409996841447155
>500	9.367870404697785E-4	0.30463907681003727
>1k	3.747148161879114E-4	0.1976786727284897
>5k	1.873574080939557E-4	0.7778002124952239
>10k+	1.873574080939557E-4	0.8459379513916171
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10677	0.8339575452576427	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9817	0.7667847917761804	No Hit
CGGTCGGAGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	1347	0.10521127783666243	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.038038524355200144	0.0
2	0.0	0.0	0.0	0.12255122117722593	0.0
3	0.0	0.0	0.0	0.2231541356936485	0.0
4	0.0	0.0	0.0	0.3296932470293631	0.0
5	0.0	0.0	0.0	0.5952599468397953	0.0
6	0.0	0.0	0.0	1.1218630909933054	0.0
7	0.0	0.0	0.0	1.4969370005490983	0.0
8	3.12431411541685E-4	0.0	0.0	2.230213523437433	0.0
9	3.12431411541685E-4	0.0	0.0	3.102834455873359	0.0
10	3.12431411541685E-4	0.0	0.0	4.091679873402792	0.0
11	3.12431411541685E-4	0.0	0.0	4.660383150261544	0.0
12	3.12431411541685E-4	0.0	0.0	5.1078630394421225	0.0
13	3.12431411541685E-4	0.0	0.0	5.381240524541097	0.0
14	3.12431411541685E-4	0.0	0.0	5.534488131902293	0.0
15	3.12431411541685E-4	0.0	0.0	5.655008548904498	0.0
16	3.12431411541685E-4	0.0	0.0	5.831141757161123	0.0
17	3.12431411541685E-4	0.0	0.0	6.010243063827394	0.0
18	3.12431411541685E-4	0.0	0.0	6.309396140378557	0.0
19	3.12431411541685E-4	0.0	0.0	6.455692148832951	0.0
20	3.12431411541685E-4	0.0	0.0	6.6363556125569305	0.0
21	3.12431411541685E-4	7.810785288542125E-5	0.0	6.804834251230784	0.0
22	3.12431411541685E-4	7.810785288542125E-5	0.0	6.982685832250889	0.0
23	3.12431411541685E-4	7.810785288542125E-5	0.0	7.193264603629984	0.0
24	3.12431411541685E-4	7.810785288542125E-5	0.0	7.36291486009712	0.0
25	3.905392644271062E-4	7.810785288542125E-5	0.0	7.50397764240819	0.0
26	3.905392644271062E-4	7.810785288542125E-5	0.0	7.646446366071198	0.0
27	3.905392644271062E-4	7.810785288542125E-5	0.0	7.809535562895958	0.0
28	3.905392644271062E-4	7.810785288542125E-5	0.0	7.972468544014947	0.0
29	3.905392644271062E-4	7.810785288542125E-5	0.0	8.140869074835916	0.0
30	4.6864711731252746E-4	7.810785288542125E-5	0.0	8.359883494326636	0.0
31	4.6864711731252746E-4	7.810785288542125E-5	0.0	8.534610761231324	0.0
32	4.6864711731252746E-4	7.810785288542125E-5	0.0	8.710743969487948	0.0
33	4.6864711731252746E-4	7.810785288542125E-5	0.0	8.891173109653272	0.0
34	5.467549701979488E-4	7.810785288542125E-5	0.0	9.067306317909896	0.0
35	5.467549701979488E-4	7.810785288542125E-5	0.0	9.276322932231283	0.0
36	5.467549701979488E-4	7.810785288542125E-5	0.0	9.46917122100539	0.0
37	5.467549701979488E-4	7.810785288542125E-5	0.0	9.676860001827723	0.0
38	5.467549701979488E-4	7.810785288542125E-5	0.0	9.943988858695864	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGAA	30	2.5279442E-6	44.0	20
TCGTAGA	30	2.5279442E-6	44.0	2
TACTCGC	20	7.8568177E-4	44.0	25
TACGTAG	50	2.7284841E-11	44.0	1
CGTTTTT	11970	0.0	43.09942	1
CGGTCTA	190	0.0	42.842106	31
GTTTTTA	6445	0.0	40.79131	2
AGCGATA	210	0.0	39.809525	8
GAGCGAT	810	0.0	39.111115	7
TGCGTTG	45	2.3512257E-8	39.11111	1
CGACGGT	215	0.0	38.88372	28
CTATCTC	705	0.0	38.695038	6
TACGGGA	160	0.0	38.5	4
GCGTAAG	75	0.0	38.13333	1
TCACGAC	220	0.0	38.000004	25
GCGCGAC	925	0.0	37.816216	9
GCGATAT	140	0.0	37.714287	9
AGCGCGA	475	0.0	37.51579	8
TTATTTT	7100	0.0	37.46197	6
CGTAAGA	100	0.0	37.4	2
>>END_MODULE