FastQCFastQC Report
Thu 26 May 2016
SRR1546475_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546475_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1592860
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96300.6045729065956833No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89160.5597478748917042No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG207.857399E-444.01
CGTTTTT123350.043.250911
TATAGCG1050.041.9047621
ATTAGCG750.041.0666661
GAGCGAT9800.039.9591837
GCGTAAG1050.039.8095251
GCGATAT950.039.368429
TCGACGG452.3519533E-839.111112
GTTTTTA70050.038.9750182
TCGATTG850.038.8235321
GCGCGAC3700.038.6486479
CGAACGC404.1258681E-738.56
CGACGGT2400.038.528
AGCGATC1500.038.133338
ATACGAG2600.038.0769233
TGTACGA1100.038.02
TTAGCGA1800.037.888892
CGGTCTA2500.037.8431
GTTGCGA700.037.7142832
TTGTGCG1350.037.4814831
Produced by FastQC (version 0.11.5)