##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546475_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1592860
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.557484022450183	31.0	31.0	34.0	30.0	34.0
2	31.934829802995868	31.0	31.0	34.0	30.0	34.0
3	32.00735155632008	33.0	31.0	34.0	30.0	34.0
4	35.70956079002549	37.0	35.0	37.0	35.0	37.0
5	35.661975314842486	37.0	35.0	37.0	33.0	37.0
6	35.71211343118667	37.0	35.0	37.0	33.0	37.0
7	36.08431061110204	37.0	35.0	37.0	35.0	37.0
8	18.24956430571425	26.0	0.0	37.0	0.0	37.0
9	27.839486835001193	25.0	17.0	39.0	17.0	39.0
10	33.62822721394222	32.0	32.0	39.0	27.0	39.0
11	35.79135077784614	37.0	35.0	39.0	32.0	39.0
12	36.58345303416496	37.0	35.0	39.0	33.0	39.0
13	36.798482603618645	39.0	35.0	39.0	33.0	39.0
14	37.98411536481549	40.0	37.0	41.0	33.0	41.0
15	38.21821001217935	40.0	37.0	41.0	33.0	41.0
16	38.273739688359306	40.0	37.0	41.0	34.0	41.0
17	38.23666549477041	40.0	37.0	41.0	33.0	41.0
18	38.170327586856345	40.0	37.0	41.0	33.0	41.0
19	38.15582725412152	40.0	37.0	41.0	34.0	41.0
20	38.042246650678656	40.0	36.0	41.0	34.0	41.0
21	37.91993583868011	40.0	36.0	41.0	33.0	41.0
22	37.92451816229926	40.0	36.0	41.0	33.0	41.0
23	37.76559333525859	39.0	36.0	41.0	33.0	41.0
24	37.58456361513253	39.0	35.0	41.0	33.0	41.0
25	37.452845824491796	39.0	35.0	41.0	33.0	41.0
26	37.413635222178975	39.0	35.0	41.0	33.0	41.0
27	37.27747950227892	39.0	35.0	41.0	33.0	41.0
28	37.253821428123	39.0	35.0	41.0	33.0	41.0
29	37.15655424833319	39.0	35.0	41.0	32.0	41.0
30	37.03600128071519	39.0	35.0	41.0	32.0	41.0
31	36.980128824881035	39.0	35.0	41.0	31.0	41.0
32	36.6820153685823	39.0	35.0	41.0	31.0	41.0
33	36.49102180982635	39.0	35.0	41.0	30.0	41.0
34	36.301376768830906	39.0	35.0	41.0	30.0	41.0
35	36.078504702233715	39.0	35.0	41.0	29.0	41.0
36	35.93366146428437	39.0	35.0	41.0	27.0	41.0
37	35.88279760933164	39.0	35.0	41.0	27.0	41.0
38	35.79991461898723	39.0	35.0	41.0	27.0	41.0
39	35.736714463292444	39.0	35.0	41.0	26.0	41.0
40	35.66769584269804	39.0	35.0	41.0	26.0	41.0
41	35.66243423778612	39.0	35.0	41.0	26.0	41.0
42	35.500667980864606	39.0	35.0	41.0	25.0	41.0
43	35.52108471554311	39.0	35.0	41.0	25.0	41.0
44	35.284505857388595	39.0	35.0	40.0	24.0	41.0
45	35.19439812664013	38.0	35.0	40.0	24.0	41.0
46	35.20473739060558	38.0	35.0	40.0	24.0	41.0
47	35.08935750787891	38.0	34.0	40.0	23.0	41.0
48	35.07158884019939	38.0	34.0	40.0	23.0	41.0
49	35.044586467109475	38.0	34.0	40.0	24.0	41.0
50	34.44782529538064	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	1.0
13	5.0
14	10.0
15	17.0
16	53.0
17	167.0
18	427.0
19	1039.0
20	2004.0
21	3396.0
22	5456.0
23	8078.0
24	12942.0
25	19971.0
26	26777.0
27	29952.0
28	29417.0
29	30465.0
30	34365.0
31	43124.0
32	55613.0
33	73235.0
34	107944.0
35	162391.0
36	160153.0
37	246694.0
38	282050.0
39	257110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.345127632058063	23.032532677071433	28.104667076830353	17.51767261404015
2	35.15481586580114	23.833921374132064	28.541303064927238	12.46995969513956
3	27.290659568323644	24.210539532664516	35.851550042062705	12.647250856949135
4	24.38996521979333	26.365154501965023	35.133093931670075	14.111786346571575
5	21.476840400286275	30.9772359152719	33.742952927438694	13.802970757003127
6	19.05026179325239	39.51847620004269	30.814572529914745	10.616689476790176
7	84.51244930502368	2.8738872217269567	10.922114937910425	1.6915485353389499
8	42.98368971535477	50.54524565875218	5.221048930853936	1.250015695039112
9	80.12072624084979	4.735130519945256	12.404291651494795	2.739851587710157
10	37.450811747422875	34.61339979659229	17.471152518112078	10.464635937872757
11	29.795148349509688	22.922165162035586	32.37855178735106	14.904134701103674
12	28.2523260047964	21.846176060670743	32.66445261981593	17.237045314716926
13	21.182338686388004	28.74414575041121	33.51487261906257	16.558642944138217
14	16.827153673266952	31.993772208480344	33.754378915912255	17.424695202340445
15	16.16174679507301	25.638034729982547	42.235224690179926	15.964993784764511
16	18.703966450284394	23.147797044310234	40.62560425900582	17.522632246399557
17	18.62699797847896	23.57212812174328	33.539733561016035	24.261140338761724
18	20.364878269276648	24.553695867810102	35.710357470210816	19.371068392702433
19	23.57451376768831	26.511871727584346	31.113280514295045	18.800333990432307
20	24.71692427457529	25.477317529475286	30.74181032859134	19.063947867358085
21	22.39801363584998	26.803736674911793	32.338372487224234	18.45987720201399
22	21.343432567833958	23.679984430521202	32.36963700513542	22.606945996509424
23	18.67797546551486	28.317868488128273	32.09227427394749	20.911881772409377
24	18.833481913036927	25.36337154552189	37.21582562183745	18.58732091960373
25	18.84051329055912	27.168866064814235	34.27777708022048	19.712843564406164
26	18.519706691109075	30.410331102545108	31.550481523799956	19.51948068254586
27	19.011463656567432	27.85825496277137	33.26827216453424	19.862009216126967
28	17.821340230779857	26.710068681491155	37.048767625528924	18.419823462200068
29	18.48555428600128	24.7793905302412	36.21159423929284	20.523460944464674
30	20.297891842346473	25.751729593310145	33.82400210941326	20.126376454930124
31	23.944602789950153	24.999748879374206	30.1069146064312	20.94873372424444
32	24.200871388571503	26.011388320379698	30.59584646484939	19.191893826199415
33	22.54021069020504	25.840563514684277	30.053237572668028	21.565988222442652
34	19.679821202114436	26.11842848712379	31.81679494745301	22.384955363308766
35	19.10688949436862	25.747334982358776	32.26353854073805	22.88223698253456
36	22.87030875280941	26.130105596223146	30.342779654206897	20.656805996760543
37	19.881596624938787	28.056138015895936	31.49950403676406	20.562761322401215
38	19.390279120575567	29.874879148198836	29.33415366071092	21.400688070514672
39	19.78284343884585	28.25320492698668	30.173963813517823	21.78998782064965
40	21.4862574237535	26.553181070527227	30.343909697022966	21.616651808696307
41	18.819293597679646	25.787137601546906	30.965935487111235	24.427633313662216
42	21.038446567808847	27.53543939831498	28.445626106500256	22.980487927375915
43	20.69792699923408	26.708122496641263	29.884107831196715	22.70984267292794
44	20.785379757166357	28.433572316462215	30.17308489132755	20.607963035043884
45	19.620431174114486	29.389023517446606	28.69021759602225	22.30032771241666
46	21.53208693796065	28.612998003591024	28.997087000740805	20.85782805770752
47	21.405333802091835	26.420275479326495	29.61063746970857	22.5637532488731
48	20.927639591677863	25.293371671082205	30.984204512637646	22.79478422460229
49	20.754805820976106	24.57315771630903	31.769395929334653	22.902640533380207
50	18.947992918398352	27.762703564657283	30.830707030121918	22.458596486822447
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	366.0
1	896.0
2	1426.0
3	15904.0
4	30382.0
5	21476.0
6	12570.0
7	12503.0
8	12436.0
9	12762.5
10	13089.0
11	13011.5
12	12934.0
13	12593.5
14	12253.0
15	11624.5
16	10996.0
17	10568.0
18	10140.0
19	9917.0
20	9694.0
21	9846.5
22	9999.0
23	11077.0
24	12155.0
25	14131.5
26	16108.0
27	19149.0
28	22190.0
29	26273.5
30	30357.0
31	35000.5
32	39644.0
33	44203.0
34	48762.0
35	53850.0
36	58938.0
37	65864.0
38	72790.0
39	83962.0
40	95134.0
41	106774.0
42	118414.0
43	126144.5
44	133875.0
45	138166.5
46	142458.0
47	144581.5
48	146705.0
49	138151.5
50	129598.0
51	114472.0
52	99346.0
53	87634.5
54	75923.0
55	67696.5
56	59470.0
57	54208.5
58	48947.0
59	43368.5
60	37790.0
61	32400.0
62	27010.0
63	22021.5
64	17033.0
65	13405.0
66	9777.0
67	7767.5
68	5758.0
69	4602.5
70	3447.0
71	2921.0
72	2395.0
73	1895.5
74	1396.0
75	964.0
76	532.0
77	417.0
78	302.0
79	234.5
80	167.0
81	141.0
82	115.0
83	65.5
84	16.0
85	16.5
86	17.0
87	10.0
88	3.0
89	3.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1592860.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.25382360103562
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.79332008397817	25.309890419732543
2	7.527626606971445	5.307552410732672
3	3.71628104606739	3.930393493497957
4	2.555179759457667	3.603194260354288
5	1.9417516656714144	3.4227085349298565
6	1.6058909553343124	3.396827787711265
7	1.3491970052093902	3.3295047257288166
8	1.2018498644139304	3.3895842491981827
9	1.0167383575521527	3.225952323499525
>10	7.2307055170336145	41.4223950502494
>50	0.04304527550876131	0.9852668465238819
>100	0.01694076326371128	1.1632901855491213
>500	9.206872112590042E-4	0.232527281866323
>1k	1.8413744225180084E-4	0.07698977004284173
>5k	3.682748845036017E-4	1.203922660383257
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9630	0.6045729065956833	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8916	0.5597478748917042	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03182953931921198	0.0
2	0.0	0.0	0.0	0.11526436723880316	0.0
3	0.0	0.0	0.0	0.1975063721858795	0.0
4	0.0	0.0	0.0	0.30912949035068993	0.0
5	0.0	0.0	0.0	0.5534698592468893	0.0
6	0.0	0.0	0.0	0.9917381314114235	0.0
7	0.0	0.0	0.0	1.2984191956606357	0.0
8	0.0	0.0	0.0	1.9511444822520498	0.0
9	0.0	0.0	0.0	2.6291073917356202	0.0
10	0.0	0.0	0.0	3.414863829840664	0.0
11	0.0	0.0	0.0	3.868262119709202	0.0
12	0.0	0.0	0.0	4.291651494795525	0.0
13	0.0	0.0	0.0	4.5100636590786385	0.0
14	0.0	0.0	0.0	4.628655374609194	0.0
15	0.0	0.0	0.0	4.7268435392941	0.0
16	0.0	0.0	0.0	4.904009140790778	0.0
17	0.0	0.0	0.0	5.106789046118303	0.0
18	0.0	0.0	0.0	5.407380435192045	0.0
19	0.0	0.0	0.0	5.559496754265911	0.0
20	0.0	0.0	0.0	5.745577137978228	0.0
21	0.0	0.0	0.0	5.936742714362844	0.0
22	0.0	0.0	0.0	6.141217683914468	0.0
23	0.0	0.0	0.0	6.3704908152631115	0.0
24	0.0	0.0	0.0	6.548409778637168	0.0
25	0.0	0.0	0.0	6.705987971322025	0.0
26	0.0	0.0	0.0	6.856409226171792	0.0
27	0.0	0.0	0.0	7.015368582298507	0.0
28	0.0	0.0	0.0	7.191341360822671	0.0
29	0.0	0.0	0.0	7.387027108471554	0.0
30	0.0	0.0	0.0	7.625905603756765	0.0
31	0.0	0.0	0.0	7.822846954534611	0.0
32	0.0	0.0	0.0	8.019286064060871	0.0
33	0.0	0.0	0.0	8.221814848762603	0.0
34	0.0	0.0	0.0	8.421518526424167	0.0
35	0.0	0.0	0.0	8.644827542910237	0.0
36	0.0	0.0	0.0	8.846037944326557	0.0
37	0.0	0.0	0.0	9.056979269992341	0.0
38	0.0	0.0	0.0	9.285499039463607	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGCG	20	7.857399E-4	44.0	1
CGTTTTT	12335	0.0	43.25091	1
TATAGCG	105	0.0	41.904762	1
ATTAGCG	75	0.0	41.066666	1
GAGCGAT	980	0.0	39.959183	7
GCGTAAG	105	0.0	39.809525	1
GCGATAT	95	0.0	39.36842	9
TCGACGG	45	2.3519533E-8	39.11111	2
GTTTTTA	7005	0.0	38.975018	2
TCGATTG	85	0.0	38.823532	1
GCGCGAC	370	0.0	38.648647	9
CGAACGC	40	4.1258681E-7	38.5	6
CGACGGT	240	0.0	38.5	28
AGCGATC	150	0.0	38.13333	8
ATACGAG	260	0.0	38.076923	3
TGTACGA	110	0.0	38.0	2
TTAGCGA	180	0.0	37.88889	2
CGGTCTA	250	0.0	37.84	31
GTTGCGA	70	0.0	37.714283	2
TTGTGCG	135	0.0	37.481483	1
>>END_MODULE