FastQCFastQC Report
Thu 26 May 2016
SRR1546474_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546474_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1399069
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84510.6040445467664569No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80460.5750967250364349No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG254.4433364E-544.09
CTATTCG207.857069E-444.01
TGCGTTG408.314601E-944.01
CGTTTTT112750.043.3365861
AGCGATC1000.041.8000038
AGCGATA2150.040.9302338
GAGCGAT8250.040.87
GACCGAT4400.039.59
CGGTCTA1950.039.4871831
CTTGCGA452.3515895E-839.111112
ACTACGG452.3515895E-839.111112
TTCGCGA452.3515895E-839.111112
GTTGCGA452.3515895E-839.111112
GTTTTTA65700.039.0776252
CGAGACC1250.038.726
CGACGGT2050.038.63414828
CACGACG2050.038.63414826
CGATTCG404.1253952E-738.510
AACGGCG700.037.7142878
TCGACGA357.291341E-637.7142872