FastQCFastQC Report
Thu 26 May 2016
SRR1546473_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546473_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences935015
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57210.6118618417886343No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53850.575926589413004No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCC16320.1745426543959188No Hit
GAATCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTT12680.1356127976556526No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATACCGCATCGTATG12460.13325989422629583No Hit
GAATCTGACTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTT11650.1245969316000278No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGG207.855728E-444.02
TCCGCGA207.855728E-444.02
TATGCGA351.4456964E-744.02
TAAGCGA551.8189894E-1244.02
TATCGCG207.855728E-444.01
CGTTTTT65250.043.022221
CGTTAGA557.8216544E-1140.02
AATGCGA501.3478711E-939.6000022
AATGCGG900.039.1111072
CGGTCTA1800.039.11110731
GTAACGA452.3501343E-839.1111072
GTTGCGA452.3501343E-839.1111072
GTTTTTA38250.038.938562
GCGCGAC5250.038.133339
AGACCGA1450.037.9310388
TCGTAGA357.2887506E-637.7142872
CCGATGA8250.037.33333218
AGACTTA5800.036.7931068
AGCGCGA3000.036.6666688
CGGGATA1500.036.6666686
Produced by FastQC (version 0.11.5)