##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546473_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	935015
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.534275920707156	31.0	31.0	34.0	30.0	34.0
2	31.91555215691727	31.0	31.0	34.0	30.0	34.0
3	31.99557333304813	33.0	31.0	34.0	30.0	34.0
4	35.708505211146345	37.0	35.0	37.0	35.0	37.0
5	35.657837574798265	37.0	35.0	37.0	33.0	37.0
6	35.70131495216654	37.0	35.0	37.0	33.0	37.0
7	36.02447661267466	37.0	35.0	37.0	35.0	37.0
8	18.222285203980686	26.0	0.0	37.0	0.0	37.0
9	27.801489815671406	25.0	17.0	39.0	17.0	39.0
10	33.5918856916734	32.0	32.0	39.0	27.0	39.0
11	35.78924509232472	37.0	35.0	39.0	32.0	39.0
12	36.5400918701839	37.0	35.0	39.0	33.0	39.0
13	36.72701614412603	39.0	35.0	39.0	33.0	39.0
14	37.90137163574916	40.0	37.0	41.0	33.0	41.0
15	38.12608995577611	40.0	37.0	41.0	33.0	41.0
16	38.200585017352665	40.0	37.0	41.0	33.0	41.0
17	38.130393630048715	40.0	37.0	41.0	33.0	41.0
18	38.07584477254375	40.0	37.0	41.0	33.0	41.0
19	38.06584707197211	40.0	37.0	41.0	34.0	41.0
20	37.891674465115535	40.0	36.0	41.0	33.0	41.0
21	37.803091929006484	40.0	36.0	41.0	33.0	41.0
22	37.7908343716411	40.0	36.0	41.0	33.0	41.0
23	37.598934776447436	39.0	36.0	41.0	33.0	41.0
24	37.45260450367106	39.0	35.0	41.0	33.0	41.0
25	37.356830639080655	39.0	35.0	41.0	33.0	41.0
26	37.29790965920333	39.0	35.0	41.0	33.0	41.0
27	37.14026192093175	39.0	35.0	41.0	32.0	41.0
28	37.12112532954017	39.0	35.0	41.0	32.0	41.0
29	36.977138334679125	39.0	35.0	41.0	32.0	41.0
30	36.87130901643289	39.0	35.0	40.0	31.0	41.0
31	36.842056009796636	39.0	35.0	41.0	31.0	41.0
32	36.50983139307925	39.0	35.0	41.0	30.0	41.0
33	36.2890691593183	39.0	35.0	41.0	30.0	41.0
34	36.07101062549798	39.0	35.0	41.0	29.0	41.0
35	35.82754501264686	39.0	35.0	41.0	27.0	41.0
36	35.710482719528564	39.0	35.0	41.0	26.0	41.0
37	35.635287134430996	39.0	35.0	41.0	26.0	41.0
38	35.55387881477837	39.0	35.0	41.0	25.0	41.0
39	35.45948353769726	39.0	35.0	41.0	25.0	41.0
40	35.41060945546328	39.0	35.0	40.0	25.0	41.0
41	35.37625599589311	39.0	35.0	40.0	24.0	41.0
42	35.217978321203404	38.0	35.0	40.0	24.0	41.0
43	35.22972251782057	38.0	35.0	40.0	24.0	41.0
44	34.99918183130752	38.0	34.0	40.0	23.0	41.0
45	34.92022160072298	38.0	34.0	40.0	23.0	41.0
46	34.894503296738556	38.0	34.0	40.0	23.0	41.0
47	34.74862007561376	38.0	34.0	40.0	23.0	41.0
48	34.74695593118827	38.0	34.0	40.0	23.0	41.0
49	34.725660016149476	38.0	34.0	40.0	23.0	41.0
50	34.13099575942632	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	0.0
12	6.0
13	5.0
14	4.0
15	14.0
16	50.0
17	95.0
18	272.0
19	594.0
20	1137.0
21	2286.0
22	3744.0
23	5739.0
24	8548.0
25	12538.0
26	16442.0
27	17953.0
28	17756.0
29	18229.0
30	20985.0
31	26201.0
32	33420.0
33	44464.0
34	66988.0
35	99098.0
36	96255.0
37	145653.0
38	159383.0
39	137152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.18246231343882	22.637711694464794	26.93058400132618	17.2492419907702
2	34.97034806928231	24.594043945819053	28.02479104613295	12.410816938765688
3	27.826933257755222	25.220878809430864	34.19838184414154	12.753806088672373
4	24.792329534820297	25.685898087196463	35.163927851424845	14.357844526558397
5	23.391496393105992	29.968610129249264	32.258412966636904	14.381480511007844
6	19.26257867520842	40.090052031250835	29.960695817714157	10.686673475826591
7	82.85963326791548	3.3027277637257155	12.027079779468778	1.8105591888900177
8	42.08937824526879	51.32677015876751	5.250076201986065	1.3337753939776367
9	78.51360673358181	4.6799249209905724	12.195312374667786	4.611155970759827
10	40.37614369822944	29.805831992000126	17.895969583375667	11.922054726394764
11	33.4161484040363	22.008951728047144	29.150976187547794	15.423923680368764
12	29.517922172371563	20.39143757052026	31.128912370389777	18.961727886718393
13	23.152890595338043	26.89582520066523	32.72246969299958	17.22881451099715
14	18.012652203440588	31.142388090030643	32.64118757453089	18.203772131997884
15	16.992454666502677	23.514275172056063	41.15495473334652	18.338315428094738
16	19.72599370063582	21.396769035790868	39.1772324508163	19.70000481275701
17	20.239889199638508	22.5469110121228	32.02847013149522	25.184729656743478
18	21.480404057688915	23.971059287818914	33.856462195793654	20.692074458698524
19	24.663240696673316	25.69359849841981	29.398779698721413	20.244381106185465
20	26.81721683609354	24.646770372667817	28.88777185392748	19.648240937311165
21	23.432458302807976	27.094003839510595	31.029448725421517	18.44408913225991
22	21.967241167254002	22.478783762827334	32.04194585113608	23.512029218782587
23	20.74490783570317	27.17154270252349	30.86228563178131	21.221263829992033
24	20.326839676368827	25.518841943712133	34.489821018914135	19.664497361004905
25	20.454431212333493	26.19444607840516	32.7637524531692	20.58737025609215
26	18.897343892878723	28.396549787971313	31.245594990454702	21.46051132869526
27	21.02169483912023	25.893381389603377	32.34857194804361	20.73635182323278
28	18.894456238669967	26.0923086795399	35.96904862488837	19.04418645690176
29	19.58193184066566	24.975000401063085	35.20713571440031	20.235932043870957
30	21.04137366780212	26.238402592471775	33.09583268717614	19.624391052549957
31	25.285476703582294	24.100468976433532	28.945845788570235	21.668208531413935
32	25.46280006203109	26.54609819093811	28.403287647791746	19.58781409923905
33	24.26196371181211	24.887515173553364	29.002850221654196	21.847670892980325
34	20.410795548734512	27.006197761533237	29.74647465548681	22.83653203424544
35	20.83282086383641	25.545151682058574	30.73672614877836	22.885301305326653
36	24.251054795912367	26.996893097971693	27.377421752592205	21.37463035352374
37	21.675695042325525	28.156018887397526	28.159655192697443	22.008630877579506
38	21.566819783639836	29.162205953915176	25.945786965984503	23.325187296460484
39	21.76638877451164	27.95773329839628	26.887162238038965	23.388715689053114
40	22.29515034518163	25.125158419918396	28.58210830842286	23.997582926477115
41	20.273685448896543	25.126655722100715	28.575263498446553	26.024395330556192
42	21.573022892680864	27.331647085875627	26.735506917001334	24.359823104442174
43	21.430351384737143	26.76673636251825	27.559450917899714	24.243461334844895
44	21.55462746586953	27.239883852130713	29.69845403549676	21.507034646502998
45	20.77485387934953	28.138906862456754	27.642872039486	23.44336721870772
46	23.27802227771747	27.274749602947544	28.361042336219207	21.08618578311578
47	21.646711550082085	26.343641545857555	29.0718330721967	22.93781383186366
48	21.883499195200077	25.10227108656011	30.083367646508346	22.930862071731468
49	21.405004197793616	24.012021197520895	31.026240220745123	23.556734383940363
50	20.080747367689288	26.55508200403202	30.420046737218122	22.94412389106057
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	228.0
1	611.0
2	994.0
3	9648.5
4	18303.0
5	12715.0
6	7127.0
7	7041.0
8	6955.0
9	7093.5
10	7232.0
11	7082.0
12	6932.0
13	6745.0
14	6558.0
15	6176.5
16	5795.0
17	5444.0
18	5093.0
19	4982.0
20	4871.0
21	5043.5
22	5216.0
23	5634.5
24	6053.0
25	7088.0
26	8123.0
27	9338.0
28	10553.0
29	12340.0
30	14127.0
31	16296.5
32	18466.0
33	20509.0
34	22552.0
35	25186.0
36	27820.0
37	31663.5
38	35507.0
39	41270.5
40	47034.0
41	54210.5
42	61387.0
43	65994.5
44	70602.0
45	76100.0
46	81598.0
47	81292.0
48	80986.0
49	77107.0
50	73228.0
51	66352.0
52	59476.0
53	54424.5
54	49373.0
55	46300.5
56	43228.0
57	40783.0
58	38338.0
59	35547.0
60	32756.0
61	29446.0
62	26136.0
63	22290.0
64	18444.0
65	15203.0
66	11962.0
67	9962.5
68	7963.0
69	6911.5
70	5860.0
71	4927.5
72	3995.0
73	3128.0
74	2261.0
75	1650.5
76	1040.0
77	757.0
78	474.0
79	338.5
80	203.0
81	141.5
82	80.0
83	56.0
84	32.0
85	32.0
86	32.0
87	22.5
88	13.0
89	9.5
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	935015.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.88298611775805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.73806981933677	27.015964982483347
2	8.942455480930482	7.133036556092397
3	5.232919911450223	6.261134165511267
4	3.848933298491255	6.140278132476137
5	2.9213999581984016	5.82570769886229
6	2.344781674165461	5.611013697594873
7	1.8239568162014705	5.09213910659674
8	1.5542223513910096	4.958962277154953
9	1.1926751122108936	4.281070044897235
>10	4.3577539555169515	23.71224650579822
>50	0.02128163342255527	0.5997232203533289
>100	0.019125614972376904	1.3819330248228716
>500	8.081245707178566E-4	0.22303451356468748
>1k	0.0010774994276238088	0.570585887062647
>5k	5.387497138119044E-4	1.1931701867290072
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5721	0.6118618417886343	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5385	0.575926589413004	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCC	1632	0.1745426543959188	No Hit
GAATCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTT	1268	0.1356127976556526	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATACCGCATCGTATG	1246	0.13325989422629583	No Hit
GAATCTGACTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTT	1165	0.1245969316000278	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.021496981331850292	0.0
2	0.0	0.0	0.0	0.09860804372122373	0.0
3	0.0	0.0	0.0	0.1727245017459613	0.0
4	0.0	0.0	0.0	0.30288284145174144	0.0
5	0.0	0.0	0.0	0.6227707576883793	0.0
6	0.0	0.0	0.0	0.9877916397063149	0.0
7	0.0	0.0	0.0	1.2417982599209638	0.0
8	0.0	0.0	0.0	1.7775115907231436	0.0
9	0.0	0.0	0.0	2.3413528125217242	0.0
10	0.0	0.0	0.0	3.1517141436233644	0.0
11	0.0	0.0	0.0	3.546894969599418	0.0
12	0.0	0.0	0.0	4.012448998144415	0.0
13	0.0	0.0	0.0	4.205814879975188	0.0
14	0.0	0.0	0.0	4.300893568552376	0.0
15	0.0	0.0	0.0	4.4065603225616705	0.0
16	0.0	0.0	0.0	4.564311802484452	0.0
17	0.0	0.0	0.0	4.727945540980626	0.0
18	0.0	0.0	0.0	4.984198114468752	0.0
19	0.0	0.0	0.0	5.1601311208911085	0.0
20	0.0	0.0	0.0	5.3114655914611	0.0
21	0.0	0.0	0.0	5.4663294171751255	0.0
22	0.0	0.0	0.0	5.645684828585638	0.0
23	0.0	0.0	0.0	5.827393143425507	0.0
24	0.0	0.0	0.0	5.974770458227943	0.0
25	0.0	0.0	0.0	6.108672053389518	0.0
26	0.0	0.0	0.0	6.232092533274868	0.0
27	0.0	0.0	0.0	6.364496826254125	0.0
28	0.0	0.0	0.0	6.502569477495014	0.0
29	0.0	0.0	0.0	6.64235333122998	0.0
30	1.069501558798522E-4	0.0	0.0	6.835612262904873	0.0
31	1.069501558798522E-4	0.0	0.0	6.992722041892376	0.0
32	1.069501558798522E-4	0.0	0.0	7.151649973529836	0.0
33	1.069501558798522E-4	0.0	0.0	7.3099362042320175	0.0
34	1.069501558798522E-4	0.0	0.0	7.47431859381935	0.0
35	1.069501558798522E-4	0.0	0.0	7.668219226429523	0.0
36	1.069501558798522E-4	0.0	0.0	7.842761880825441	0.0
37	1.069501558798522E-4	0.0	0.0	8.02489799628883	0.0
38	1.069501558798522E-4	0.0	0.0	8.232381298695742	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGG	20	7.855728E-4	44.0	2
TCCGCGA	20	7.855728E-4	44.0	2
TATGCGA	35	1.4456964E-7	44.0	2
TAAGCGA	55	1.8189894E-12	44.0	2
TATCGCG	20	7.855728E-4	44.0	1
CGTTTTT	6525	0.0	43.02222	1
CGTTAGA	55	7.8216544E-11	40.0	2
AATGCGA	50	1.3478711E-9	39.600002	2
AATGCGG	90	0.0	39.111107	2
CGGTCTA	180	0.0	39.111107	31
GTAACGA	45	2.3501343E-8	39.111107	2
GTTGCGA	45	2.3501343E-8	39.111107	2
GTTTTTA	3825	0.0	38.93856	2
GCGCGAC	525	0.0	38.13333	9
AGACCGA	145	0.0	37.931038	8
TCGTAGA	35	7.2887506E-6	37.714287	2
CCGATGA	825	0.0	37.333332	18
AGACTTA	580	0.0	36.793106	8
AGCGCGA	300	0.0	36.666668	8
CGGGATA	150	0.0	36.666668	6
>>END_MODULE