FastQCFastQC Report
Thu 26 May 2016
SRR1546472_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546472_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1676483
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130570.7788328303955364No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124710.7438787032138113No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCT17340.10343081319643564No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG207.8575144E-444.0000041
TTTCGCG302.5284153E-644.01
TCGTGCG254.4437165E-544.01
CGTTTTT171150.043.24161
GAGCGAT9600.041.0208327
CGACGGT1450.040.9655228
TATACGG603.6379788E-1240.3333322
GTTTTTA96700.039.8821072
CGAGGTT2900.039.4482776
TATACGA900.039.111112
TGTACGA850.038.823532
GCGAGAC3800.038.7894745
CTTGCGA800.038.5000042
TCGCGGA404.12605E-738.5000042
CGAAGAG3900.038.3589743
CGTAAGA1100.038.02
CGGGTAT1050.037.7142876
CGCGCGA357.292205E-637.7142872
ATATCCG357.292205E-637.7142871
CGATCGT357.292205E-637.71428710