##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546472_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1676483
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59677610807864	31.0	31.0	34.0	30.0	34.0
2	31.971527895004005	31.0	31.0	34.0	30.0	34.0
3	32.0236954386057	33.0	31.0	34.0	30.0	34.0
4	35.73387204045612	37.0	35.0	37.0	35.0	37.0
5	35.68583516802735	37.0	35.0	37.0	33.0	37.0
6	35.728605061906386	37.0	35.0	37.0	33.0	37.0
7	36.10074662254255	37.0	35.0	37.0	35.0	37.0
8	18.131137029125856	18.0	0.0	37.0	0.0	37.0
9	27.77484173713661	19.0	17.0	39.0	17.0	39.0
10	33.642531418451604	32.0	32.0	39.0	27.0	39.0
11	35.806274802667254	37.0	35.0	39.0	32.0	39.0
12	36.65407463123694	38.0	35.0	39.0	33.0	39.0
13	36.90270584312516	39.0	37.0	39.0	33.0	39.0
14	38.12513935423145	40.0	37.0	41.0	33.0	41.0
15	38.33499355495999	40.0	37.0	41.0	33.0	41.0
16	38.38718257208692	40.0	37.0	41.0	34.0	41.0
17	38.348723488398036	40.0	37.0	41.0	34.0	41.0
18	38.27046441866693	40.0	37.0	41.0	34.0	41.0
19	38.24173940326266	40.0	37.0	41.0	34.0	41.0
20	38.102914255617264	40.0	36.0	41.0	34.0	41.0
21	37.98748928560564	40.0	36.0	41.0	33.0	41.0
22	37.990707331956244	40.0	36.0	41.0	33.0	41.0
23	37.81341057439891	39.0	36.0	41.0	33.0	41.0
24	37.583560942759334	39.0	35.0	41.0	33.0	41.0
25	37.453380081993075	39.0	35.0	41.0	33.0	41.0
26	37.414551176480764	39.0	35.0	41.0	33.0	41.0
27	37.27515280500906	39.0	35.0	41.0	33.0	41.0
28	37.23763438102265	39.0	35.0	41.0	33.0	41.0
29	37.130185632660755	39.0	35.0	41.0	32.0	41.0
30	36.97758044668512	39.0	35.0	41.0	32.0	41.0
31	36.88463169623551	39.0	35.0	41.0	31.0	41.0
32	36.52085407367686	39.0	35.0	41.0	30.0	41.0
33	36.231987440373686	39.0	35.0	41.0	30.0	41.0
34	35.9614407065267	39.0	35.0	41.0	27.0	41.0
35	35.71154136367622	39.0	35.0	41.0	25.0	41.0
36	35.56007367805101	39.0	35.0	41.0	24.0	41.0
37	35.49870950078229	39.0	35.0	41.0	24.0	41.0
38	35.42850896788097	39.0	35.0	41.0	23.0	41.0
39	35.358576257558234	39.0	35.0	41.0	23.0	41.0
40	35.303724523302655	39.0	35.0	41.0	23.0	41.0
41	35.28165451125958	39.0	35.0	41.0	23.0	41.0
42	35.12457806014138	39.0	35.0	41.0	23.0	41.0
43	35.14463015729954	39.0	35.0	41.0	23.0	41.0
44	34.909727089388916	39.0	35.0	40.0	21.0	41.0
45	34.83664850761982	38.0	34.0	40.0	21.0	41.0
46	34.84362084196499	38.0	34.0	40.0	22.0	41.0
47	34.74013753792911	38.0	34.0	40.0	20.0	41.0
48	34.72497722911595	38.0	34.0	40.0	21.0	41.0
49	34.69880994916143	38.0	34.0	40.0	20.0	41.0
50	34.11484936023807	38.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	1.0
13	2.0
14	8.0
15	24.0
16	61.0
17	184.0
18	483.0
19	1113.0
20	2256.0
21	3805.0
22	6167.0
23	9653.0
24	15581.0
25	24268.0
26	33516.0
27	36936.0
28	34714.0
29	33595.0
30	36698.0
31	44689.0
32	56968.0
33	74906.0
34	108926.0
35	161514.0
36	164560.0
37	255482.0
38	295612.0
39	274756.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.122584004728946	22.674253183599237	27.46380368903234	19.739359122639478
2	36.82524666220892	23.16426709963656	27.634041025169953	12.376445212984564
3	26.126480256584767	23.574530728912848	37.81899369095899	12.479995323543394
4	23.436921221390257	25.849173537697666	36.995901539114925	13.718003701797155
5	21.210593844375396	30.053331885858668	35.434776254814395	13.301298014951538
6	18.736068304897813	38.154756117419616	32.69057902764299	10.418596550039577
7	81.71773886165263	2.87065243131007	13.493545714451027	1.9180629925862656
8	41.31476430121868	50.83314295462584	6.515723690607063	1.3363690535484105
9	77.03931384929045	4.780722500615873	15.209161083053036	2.970802567040644
10	36.42882152697045	33.404394795533264	20.159464784313354	10.007318893182932
11	26.487175831785947	22.95770371664968	36.22303357683913	14.332086874725244
12	25.821436900940835	21.679969316718392	35.17339573380702	17.325198048533746
13	20.005093997374264	26.98243883176865	36.45679675845207	16.555670412405014
14	15.930552233455394	30.236930526584523	36.349906321746175	17.482610918213904
15	15.585782856133942	25.327426523263284	43.30893901101294	15.777851609589838
16	18.298068038864695	23.66603180586979	41.47456311814674	16.56133703711878
17	18.195770550611012	23.847483094072533	35.58688039186798	22.369865963448483
18	19.3549233723217	24.918415516292143	37.55898508961916	18.167676021766997
19	22.09733113905718	26.926130476718225	33.03845013638671	17.938088247837886
20	23.293108251023124	25.593042100635678	33.20648047132002	17.90736917702118
21	20.875129661320756	26.92702520693619	34.327756380470305	17.870088751272753
22	19.976522279080672	24.01098012923483	34.552810854628405	21.45968673705609
23	17.717447776088395	27.424554856804395	34.55973010164732	20.298267265459895
24	18.06179961264146	24.921159355627225	38.943430980212746	18.073610051518564
25	18.17352159252435	26.869583526943007	36.130458823620636	18.826436056911998
26	17.70241630842663	29.858519293067694	33.671561238616796	18.76750315988889
27	18.386228789674576	27.48963156799085	35.3137490806647	18.810390561669877
28	17.333190971814208	26.514673873817983	38.16447885245481	17.987656301912995
29	17.940951384535364	24.627986087541597	37.12498128522627	20.306081242696763
30	19.122770705101093	26.49731610758952	35.01795127060638	19.361961916703002
31	22.00320552012755	25.652869727876755	32.215000092455455	20.12892465954024
32	22.04901570728722	26.725889853938273	31.705481057666557	19.519613381107952
33	20.79716883499564	26.761858008700358	31.841658996840412	20.599314159463592
34	17.89883941560994	27.18548294256488	32.22430528672226	22.691372355102914
35	18.034838408740203	26.86385725354805	32.51479436415401	22.58650997355774
36	21.0754299327819	26.50065643373658	30.506125024828762	21.917788608652756
37	18.794166120384162	29.005781746668475	31.66742519906256	20.532626933884806
38	18.333618652858394	29.261972832411658	30.15616621224313	22.248242302486812
39	19.064792186977144	28.55668682593262	31.115257357217462	21.263263629872775
40	20.720162387569694	26.477572394113153	30.9104237859853	21.89184143233185
41	18.22660891879011	26.15039937774496	31.681800531231154	23.94119117223378
42	20.263671030365355	27.39502875961164	29.89096817563912	22.450332034383884
43	19.99149409806124	26.896306136119485	31.30684892122378	21.8053508445955
44	20.13679828545831	28.342369114390063	31.070282251594563	20.450550348557066
45	18.756348856504957	29.37673689503562	29.955329102651206	21.911585145808218
46	20.720520279656878	28.822183105942617	29.649569962832906	20.8077266515676
47	20.159405135632152	26.73525469688628	30.455125402404914	22.65021476507665
48	20.247804481166824	24.99977631744551	32.09439045907415	22.658028742313522
49	19.97795384742941	24.65309818232574	32.38732513243499	22.981622837809866
50	18.666458293940348	27.369737718783902	32.11711660661039	21.846687380665358
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	439.0
1	999.5
2	1560.0
3	21569.0
4	41578.0
5	29262.5
6	16947.0
7	16897.5
8	16848.0
9	17150.5
10	17453.0
11	17377.5
12	17302.0
13	16679.5
14	16057.0
15	15150.0
16	14243.0
17	13598.0
18	12953.0
19	12584.0
20	12215.0
21	12409.0
22	12603.0
23	13685.0
24	14767.0
25	16829.5
26	18892.0
27	22085.0
28	25278.0
29	29933.0
30	34588.0
31	39599.0
32	44610.0
33	50232.5
34	55855.0
35	61447.0
36	67039.0
37	74073.5
38	81108.0
39	91796.5
40	102485.0
41	113055.5
42	123626.0
43	130805.0
44	137984.0
45	144508.0
46	151032.0
47	151363.0
48	151694.0
49	140143.0
50	128592.0
51	112557.0
52	96522.0
53	84195.0
54	71868.0
55	63640.5
56	55413.0
57	50050.0
58	44687.0
59	39734.5
60	34782.0
61	29365.5
62	23949.0
63	18971.5
64	13994.0
65	10687.5
66	7381.0
67	5754.0
68	4127.0
69	3429.5
70	2732.0
71	2178.0
72	1624.0
73	1238.0
74	852.0
75	655.0
76	458.0
77	330.5
78	203.0
79	148.0
80	93.0
81	62.0
82	31.0
83	19.5
84	8.0
85	8.0
86	8.0
87	5.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1676483.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.800597495858234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.55826073333641	29.196197936845365
2	8.055822379747974	6.573647328284479
3	4.573687142705588	5.59827504544538
4	3.040695119505419	4.9624871071424455
5	2.3558469402072597	4.8059981384622805
6	1.8729547992102402	4.585060493430779
7	1.5387193209539982	4.394646737134107
8	1.2228878327185717	3.99156433962663
9	1.0292169580386756	3.7793400156762855
>10	4.709270694067321	28.106230132588227
>50	0.030554170453209607	0.8063932417083277
>100	0.009760126180557556	0.849271040849276
>500	0.001239350866523976	0.3281117927192828
>1k	7.745942915774849E-4	0.4092074255566629
>5k	0.0	0.0
>10k+	3.09837716630994E-4	1.613569224530505
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13057	0.7788328303955364	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12471	0.7438787032138113	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCT	1734	0.10343081319643564	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.964868119748307E-5	0.0	0.0	0.04264880705620039	0.0
2	5.964868119748307E-5	0.0	0.0	0.1391007245525305	0.0
3	5.964868119748307E-5	0.0	0.0	0.24288942983615103	0.0
4	5.964868119748307E-5	0.0	0.0	0.37942526109718977	0.0
5	5.964868119748307E-5	0.0	0.0	0.685065103553093	0.0
6	5.964868119748307E-5	0.0	0.0	1.246538139665001	0.0
7	5.964868119748307E-5	0.0	0.0	1.6423071394103013	0.0
8	5.964868119748307E-5	0.0	0.0	2.4012173102858783	0.0
9	5.964868119748307E-5	0.0	0.0	3.2774564370769044	0.0
10	5.964868119748307E-5	0.0	0.0	4.179165550739256	0.0
11	5.964868119748307E-5	0.0	0.0	4.682779366089606	0.0
12	5.964868119748307E-5	0.0	0.0	5.124537499038165	0.0
13	5.964868119748307E-5	0.0	0.0	5.388244318612237	0.0
14	5.964868119748307E-5	0.0	0.0	5.532892370516134	0.0
15	5.964868119748307E-5	0.0	0.0	5.647596784458894	0.0
16	5.964868119748307E-5	0.0	0.0	5.827616504312898	0.0
17	5.964868119748307E-5	0.0	0.0	6.034478130705769	0.0
18	5.964868119748307E-5	0.0	0.0	6.359205551144867	0.0
19	5.964868119748307E-5	0.0	0.0	6.52335872180034	0.0
20	5.964868119748307E-5	0.0	0.0	6.715248529212643	0.0
21	5.964868119748307E-5	0.0	0.0	6.901113819824	0.0
22	5.964868119748307E-5	0.0	0.0	7.108273689622859	0.0
23	5.964868119748307E-5	0.0	0.0	7.33267202828779	0.0
24	5.964868119748307E-5	0.0	0.0	7.506428636616059	0.0
25	5.964868119748307E-5	0.0	0.0	7.667122183762078	0.0
26	5.964868119748307E-5	0.0	0.0	7.818808780047277	0.0
27	5.964868119748307E-5	0.0	0.0	7.986242628168612	0.0
28	5.964868119748307E-5	0.0	0.0	8.167395672965368	0.0
29	5.964868119748307E-5	0.0	0.0	8.351053962372418	0.0
30	5.964868119748307E-5	0.0	0.0	8.595255663194914	0.0
31	5.964868119748307E-5	0.0	0.0	8.797941881903961	0.0
32	5.964868119748307E-5	0.0	0.0	8.989593094591475	0.0
33	5.964868119748307E-5	0.0	0.0	9.182974119033716	0.0
34	5.964868119748307E-5	0.0	0.0	9.382797201045284	0.0
35	5.964868119748307E-5	0.0	0.0	9.609402540914521	0.0
36	5.964868119748307E-5	0.0	0.0	9.816383464669787	0.0
37	5.964868119748307E-5	0.0	0.0	10.033683610272218	0.0
38	5.964868119748307E-5	0.0	0.0	10.266969602435575	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCG	20	7.8575144E-4	44.000004	1
TTTCGCG	30	2.5284153E-6	44.0	1
TCGTGCG	25	4.4437165E-5	44.0	1
CGTTTTT	17115	0.0	43.2416	1
GAGCGAT	960	0.0	41.020832	7
CGACGGT	145	0.0	40.96552	28
TATACGG	60	3.6379788E-12	40.333332	2
GTTTTTA	9670	0.0	39.882107	2
CGAGGTT	290	0.0	39.448277	6
TATACGA	90	0.0	39.11111	2
TGTACGA	85	0.0	38.82353	2
GCGAGAC	380	0.0	38.789474	5
CTTGCGA	80	0.0	38.500004	2
TCGCGGA	40	4.12605E-7	38.500004	2
CGAAGAG	390	0.0	38.358974	3
CGTAAGA	110	0.0	38.0	2
CGGGTAT	105	0.0	37.714287	6
CGCGCGA	35	7.292205E-6	37.714287	2
ATATCCG	35	7.292205E-6	37.714287	1
CGATCGT	35	7.292205E-6	37.714287	10
>>END_MODULE