FastQCFastQC Report
Thu 26 May 2016
SRR1546471_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546471_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1782168
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97880.5492187044094609No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91400.5128584959442657No Hit
CGGTCGGAGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC29370.16479927818252826No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC19080.10706061381418586No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGA650.043.9999962
CGTTTTT109350.042.853221
CGGTCTA3750.042.23999831
ACGGTCT4000.040.14999830
CGACGGT4000.039.628
TCACGAC4200.039.28571325
TCTAGCG452.3521352E-839.111111
GTTTTTA62600.038.693292
GAGCGAT9550.038.010477
CACGACG4300.037.86046626
AGACTTA16000.037.81258
TAGCCGT700.037.71428744
CTATCTC7350.037.1156466
TTACTGG30300.036.81188240
GACCGAT5150.036.737869
AGCGCGA6950.036.7194258
TAGTACG601.9826984E-1036.6666641
TTTAGCG850.036.2352941
AGCGATA2500.036.088
GCGCGAC13850.036.0577629
Produced by FastQC (version 0.11.5)