##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546471_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1782168
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5145704557595	31.0	31.0	33.0	30.0	34.0
2	31.899657608036954	31.0	31.0	34.0	30.0	34.0
3	31.990613118404102	33.0	31.0	34.0	30.0	34.0
4	35.687458196982554	37.0	35.0	37.0	35.0	37.0
5	35.65302036620565	37.0	35.0	37.0	33.0	37.0
6	35.698746695036604	37.0	35.0	37.0	33.0	37.0
7	36.01530832110104	37.0	35.0	37.0	35.0	37.0
8	18.396541740172644	30.0	0.0	37.0	0.0	37.0
9	27.889339276656298	28.0	17.0	39.0	17.0	39.0
10	33.63937294351599	32.0	32.0	39.0	27.0	39.0
11	35.84351306947493	37.0	35.0	39.0	32.0	39.0
12	36.53342333607157	37.0	35.0	39.0	33.0	39.0
13	36.72147294755601	39.0	35.0	39.0	33.0	39.0
14	37.86591107011236	40.0	36.0	41.0	33.0	41.0
15	38.083589762581305	40.0	37.0	41.0	33.0	41.0
16	38.131644715874145	40.0	37.0	41.0	33.0	41.0
17	38.072530199173144	40.0	37.0	41.0	33.0	41.0
18	38.02430578935319	40.0	37.0	41.0	33.0	41.0
19	38.02869594785677	40.0	37.0	41.0	33.0	41.0
20	37.92606140386316	40.0	36.0	41.0	33.0	41.0
21	37.80617091093544	40.0	36.0	41.0	33.0	41.0
22	37.80415651049733	40.0	36.0	41.0	33.0	41.0
23	37.669479532793765	39.0	36.0	41.0	33.0	41.0
24	37.51594911366381	39.0	35.0	41.0	33.0	41.0
25	37.37191162673777	39.0	35.0	41.0	32.0	41.0
26	37.32582337916515	39.0	35.0	41.0	33.0	41.0
27	37.213878826238606	39.0	35.0	41.0	32.0	41.0
28	37.18504035534248	39.0	35.0	41.0	32.0	41.0
29	37.040192619326575	39.0	35.0	41.0	32.0	41.0
30	36.897138765817814	39.0	35.0	40.0	31.0	41.0
31	36.88836237661096	39.0	35.0	41.0	31.0	41.0
32	36.6626154212173	39.0	35.0	41.0	31.0	41.0
33	36.492154499463574	39.0	35.0	41.0	30.0	41.0
34	36.332329499800245	39.0	35.0	41.0	30.0	41.0
35	36.09282177662263	39.0	35.0	41.0	29.0	41.0
36	35.9799850519143	39.0	35.0	41.0	29.0	41.0
37	35.94349466492497	39.0	35.0	41.0	29.0	41.0
38	35.85558432201678	39.0	35.0	41.0	28.0	41.0
39	35.80553404617297	39.0	35.0	40.0	28.0	41.0
40	35.72942786538643	39.0	35.0	40.0	27.0	41.0
41	35.70929676663479	38.0	35.0	40.0	28.0	41.0
42	35.54970575164631	38.0	35.0	40.0	27.0	41.0
43	35.56002632748428	38.0	35.0	40.0	27.0	41.0
44	35.30553011837268	38.0	34.0	40.0	26.0	41.0
45	35.199699467165836	38.0	34.0	40.0	26.0	41.0
46	35.18855966440874	38.0	34.0	40.0	26.0	41.0
47	35.049002114278785	38.0	34.0	40.0	26.0	41.0
48	35.02724097840383	38.0	34.0	40.0	26.0	41.0
49	34.97073844890044	37.0	34.0	40.0	26.0	41.0
50	34.37832348016573	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	4.0
12	3.0
13	8.0
14	21.0
15	41.0
16	88.0
17	240.0
18	487.0
19	993.0
20	2021.0
21	3298.0
22	5318.0
23	8163.0
24	12485.0
25	18648.0
26	25821.0
27	30157.0
28	32295.0
29	34749.0
30	41456.0
31	52206.0
32	66469.0
33	89161.0
34	132275.0
35	192849.0
36	191205.0
37	286938.0
38	302870.0
39	251896.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.67639863357439	22.584346705810002	27.16651853248403	16.57273612813158
2	35.574255625732256	23.428599323969458	28.094601631271576	12.902543419026713
3	29.94700836284795	24.179763075086075	32.734568233746764	13.138660328319215
4	26.648497784720632	25.947273208810838	31.902996799403873	15.501232207064655
5	22.201330065403486	30.901856615089034	31.217315090384297	15.679498229123181
6	20.473883494709813	39.662871289350946	28.878983350615655	10.984261865323584
7	84.42245624430468	3.7685560508324696	9.656048139120442	2.152939565742399
8	43.469078111603395	50.379032728676535	4.631044884657339	1.5208442750627325
9	80.36722688321191	4.815314830027248	11.226831589390002	3.5906266973708427
10	40.84036970700854	29.033682570891184	16.611453016775073	13.5144947053252
11	35.39980518110526	22.13337912026251	25.325502421769443	17.14131327686279
12	32.51197417976307	19.88157120989716	29.24679379272885	18.35966081761091
13	24.92138788262386	25.04690915783473	30.705410488797913	19.3262924707435
14	19.31877353874607	29.162402197772604	31.53131466842632	19.987509595055013
15	18.74997194428359	23.296513011119043	38.561852754622464	19.391662289974906
16	22.524026915532094	20.581729668583435	37.14425351594238	19.749989899942094
17	21.72960124971383	21.293839862459656	30.76606694767272	26.21049194015379
18	23.507772555673764	23.294773556701724	32.0752027867182	21.12225110090631
19	26.182267889447008	26.541437170906445	27.480013107630707	19.796281832015836
20	27.736610689901287	25.087758280925254	26.910987067436963	20.264643961736493
21	25.788253408208426	26.16902559130228	28.793020635540532	19.24970036494876
22	23.40587419367871	22.372021044031765	29.799210848808865	24.42289391348066
23	22.2256263158131	26.271428956192683	29.182826759317866	22.320117968676353
24	23.151464957287978	25.051173626728797	31.787351136368734	20.010010279614495
25	23.968615753396985	23.83725888917319	30.10765539500204	22.086469962427785
26	20.479438526558663	27.16287128935095	28.782078906141283	23.575611277949104
27	21.231556172033166	24.686505424853326	30.98675321294064	23.095185190172867
28	20.732781645725883	25.56016043380871	32.75987448994708	20.947183430518336
29	21.97722100273375	25.388796117986633	31.59651615335928	21.03746672592034
30	24.09986039475515	24.273356945024265	31.115697285553328	20.51108537466726
31	28.058297534239195	23.274797886618995	27.223359413927305	21.443545165214502
32	28.31068675904853	24.681455395899825	27.099577593133755	19.90828025191789
33	26.950826184736794	23.995717575447433	27.41991776308406	21.63353847673171
34	23.464398418106487	24.965154800220855	29.489195182496825	22.081251599175836
35	22.2067728743867	25.097353335936905	30.09581588267773	22.600057906998668
36	26.589300223099055	25.966463318834137	26.653379479375683	20.79085697869112
37	23.043843229145626	27.942932428368145	26.518319260585983	22.494905081900246
38	22.809802442867337	29.285791238536437	25.34783477203047	22.556571546565756
39	22.477286091995815	26.123519219287967	25.715364656979588	25.683830031736626
40	23.257683899609912	24.20809934865849	28.509433454085137	24.02478329764646
41	20.965307423318116	23.960704041369837	28.403943960389817	26.67004457492223
42	22.099824483438148	27.05715734992436	25.562573225419825	25.28044494121766
43	21.67758595149279	25.436434724447977	26.96951129186474	25.916468032194494
44	22.62401748881138	25.3944072612683	28.97055720897244	23.011018040947878
45	22.155823693389177	26.52359373527075	27.35718518119504	23.963397390145037
46	24.136388937518795	25.883306175399852	28.172820968617994	21.807483918463355
47	22.904686875760312	25.819675810585757	27.82796010252681	23.44767721112712
48	22.316077945513555	24.947816367480506	29.184902882332082	23.551202804673856
49	22.7298436511036	23.4769112676246	30.001604786978557	23.79164029429324
50	21.4694686471758	24.982324898662753	29.939489430850514	23.608717023310934
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	498.0
1	1373.0
2	2248.0
3	16617.0
4	30986.0
5	21022.5
6	11059.0
7	10885.5
8	10712.0
9	10715.0
10	10718.0
11	10493.5
12	10269.0
13	9893.0
14	9517.0
15	8976.5
16	8436.0
17	8207.0
18	7978.0
19	7664.0
20	7350.0
21	7440.0
22	7530.0
23	8196.5
24	8863.0
25	10352.0
26	11841.0
27	14112.5
28	16384.0
29	19130.5
30	21877.0
31	25341.0
32	28805.0
33	32757.5
34	36710.0
35	41569.5
36	46429.0
37	51305.0
38	56181.0
39	66050.5
40	75920.0
41	88158.0
42	100396.0
43	108302.5
44	116209.0
45	125259.5
46	134310.0
47	141716.0
48	149122.0
49	146182.5
50	143243.0
51	130097.5
52	116952.0
53	108163.5
54	99375.0
55	96560.5
56	93746.0
57	93832.0
58	93918.0
59	90684.5
60	87451.0
61	81712.5
62	75974.0
63	65126.0
64	54278.0
65	44323.0
66	34368.0
67	28925.5
68	23483.0
69	20152.5
70	16822.0
71	13836.0
72	10850.0
73	8348.5
74	5847.0
75	4303.0
76	2759.0
77	2077.5
78	1396.0
79	1103.0
80	810.0
81	576.0
82	342.0
83	246.0
84	150.0
85	90.0
86	30.0
87	20.5
88	11.0
89	9.5
90	8.0
91	5.0
92	2.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1782168.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.1318221169229
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.83544498614324	23.082037399847554
2	6.620658744971955	4.254676581805778
3	3.275482197331694	3.1574163393542922
4	2.393590404032436	3.0764168433257537
5	1.8709828176652736	3.0059043540519883
6	1.5869165028757877	3.059431127088851
7	1.4025259071615432	3.1545999074303444
8	1.2190424836649967	3.133604499047645
9	1.1491686684882965	3.323239491438637
>10	8.55658087842717	45.40928031601081
>50	0.057543840034757696	1.242595217996057
>100	0.027824477733508088	1.8708008913159595
>500	0.0029482375439215357	0.6125623748323924
>1k	9.2132423247548E-4	0.49675220615153415
>5k	3.6852969299019196E-4	1.120682450302293
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9788	0.5492187044094609	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9140	0.5128584959442657	No Hit
CGGTCGGAGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	2937	0.16479927818252826	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	1908	0.10706061381418586	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022444573126663703	0.0
2	0.0	0.0	0.0	0.07973434603247281	0.0
3	0.0	0.0	0.0	0.138819684788415	0.0
4	0.0	0.0	0.0	0.22141571389453743	0.0
5	0.0	0.0	0.0	0.4045073191752966	0.0
6	0.0	0.0	0.0	0.7705783068711816	0.0
7	0.0	0.0	0.0	0.9959218210628852	0.0
8	0.0	0.0	0.0	1.4968285818172025	0.0
9	0.0	0.0	0.0	2.0321877623209486	0.0
10	0.0	0.0	0.0	2.6854370631724955	0.0
11	0.0	0.0	0.0	3.087812147900759	0.0
12	0.0	0.0	0.0	3.4149979126546994	0.0
13	0.0	0.0	0.0	3.5826027624780603	0.0
14	0.0	0.0	0.0	3.670473266268949	0.0
15	0.0	0.0	0.0	3.7481314892872053	0.0
16	0.0	0.0	0.0	3.877805010526505	0.0
17	0.0	0.0	0.0	4.025546413132768	0.0
18	0.0	0.0	0.0	4.252573270308972	0.0
19	0.0	0.0	0.0	4.358736101198091	0.0
20	0.0	0.0	0.0	4.493235205659624	0.0
21	0.0	0.0	0.0	4.635477687849855	0.0
22	0.0	0.0	0.0	4.7905696881551005	0.0
23	0.0	0.0	0.0	4.961485112514645	0.0
24	0.0	0.0	0.0	5.104064263301776	0.0
25	0.0	0.0	0.0	5.221673826485494	0.0
26	0.0	0.0	0.0	5.335411700804863	0.0
27	0.0	0.0	0.0	5.460484084553196	0.0
28	5.611143281665926E-5	0.0	0.0	5.596722643432044	0.0
29	5.611143281665926E-5	0.0	0.0	5.735093436757927	0.0
30	5.611143281665926E-5	0.0	0.0	5.910778332906887	0.0
31	5.611143281665926E-5	0.0	0.0	6.064018655929183	0.0
32	5.611143281665926E-5	0.0	0.0	6.224946245247362	0.0
33	5.611143281665926E-5	0.0	0.0	6.3885110719079234	0.0
34	5.611143281665926E-5	0.0	0.0	6.544613078003869	0.0
35	5.611143281665926E-5	0.0	0.0	6.73842196695261	0.0
36	5.611143281665926E-5	0.0	0.0	6.901089010688105	0.0
37	5.611143281665926E-5	0.0	0.0	7.073687778032149	0.0
38	5.611143281665926E-5	0.0	0.0	7.286742888437005	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGA	65	0.0	43.999996	2
CGTTTTT	10935	0.0	42.85322	1
CGGTCTA	375	0.0	42.239998	31
ACGGTCT	400	0.0	40.149998	30
CGACGGT	400	0.0	39.6	28
TCACGAC	420	0.0	39.285713	25
TCTAGCG	45	2.3521352E-8	39.11111	1
GTTTTTA	6260	0.0	38.69329	2
GAGCGAT	955	0.0	38.01047	7
CACGACG	430	0.0	37.860466	26
AGACTTA	1600	0.0	37.8125	8
TAGCCGT	70	0.0	37.714287	44
CTATCTC	735	0.0	37.115646	6
TTACTGG	3030	0.0	36.811882	40
GACCGAT	515	0.0	36.73786	9
AGCGCGA	695	0.0	36.719425	8
TAGTACG	60	1.9826984E-10	36.666664	1
TTTAGCG	85	0.0	36.235294	1
AGCGATA	250	0.0	36.08	8
GCGCGAC	1385	0.0	36.057762	9
>>END_MODULE