FastQCFastQC Report
Thu 26 May 2016
SRR1546470_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546470_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1867073
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73030.3911469985372827No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69700.3733115952081145No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35620.19077989987536642No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCTA254.4439126E-544.013
TAAGTCG254.4439126E-544.01
CGCGAGT207.857746E-444.04
CCGGTCG207.857746E-444.012
CACGTAT302.52857E-644.040
TCGCGGA551.8189894E-1244.02
GACGGTC3400.043.35294329
CGTTTTT117900.043.104331
CGGTCTA3450.042.7246431
CGACGGT3500.042.1142928
ACGGTCT3550.041.52112630
TCACGAC3650.040.986325
GAGCGAT9850.040.87317
TAGCACG700.040.8571431
TATAGCG603.6379788E-1240.3333321
GCTTGCG557.8216544E-1140.01
GCGATCG557.8216544E-1140.09
GTTTTTA69700.039.4232442
GGTACGA950.039.3684232
TGCTACG1300.038.9230771
Produced by FastQC (version 0.11.5)