##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546470_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1867073
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.57340714583736	31.0	31.0	34.0	30.0	34.0
2	31.94170929578008	31.0	31.0	34.0	30.0	34.0
3	32.016532829728675	33.0	31.0	34.0	30.0	34.0
4	35.72062420698066	37.0	35.0	37.0	35.0	37.0
5	35.65417260064282	37.0	35.0	37.0	33.0	37.0
6	35.706938079014584	37.0	35.0	37.0	33.0	37.0
7	36.0859430777479	37.0	35.0	37.0	35.0	37.0
8	18.25534352432926	26.0	0.0	37.0	0.0	37.0
9	27.85535648579354	25.0	17.0	39.0	17.0	39.0
10	33.599903699533975	32.0	32.0	39.0	27.0	39.0
11	35.77409185393394	37.0	35.0	39.0	32.0	39.0
12	36.45889903608482	37.0	35.0	39.0	33.0	39.0
13	36.58287597753275	39.0	35.0	39.0	33.0	39.0
14	37.74718824598717	39.0	36.0	41.0	33.0	41.0
15	38.02996776237458	40.0	36.0	41.0	33.0	41.0
16	38.122191794321914	40.0	36.0	41.0	33.0	41.0
17	38.093985612774645	40.0	36.0	41.0	33.0	41.0
18	38.05445153992372	40.0	36.0	41.0	33.0	41.0
19	38.04903075562659	40.0	36.0	41.0	34.0	41.0
20	37.9177209461012	40.0	36.0	41.0	34.0	41.0
21	37.78646737433405	40.0	36.0	41.0	33.0	41.0
22	37.78276371625534	40.0	36.0	41.0	33.0	41.0
23	37.63578071130588	39.0	35.0	41.0	33.0	41.0
24	37.49015223293358	39.0	35.0	41.0	33.0	41.0
25	37.38597687396261	39.0	35.0	41.0	33.0	41.0
26	37.329267254145925	39.0	35.0	41.0	33.0	41.0
27	37.16038633733121	39.0	35.0	41.0	32.0	41.0
28	37.191619181467466	39.0	35.0	41.0	32.0	41.0
29	37.12026042902447	39.0	35.0	41.0	32.0	41.0
30	37.03176951302922	39.0	35.0	41.0	32.0	41.0
31	36.98745148154357	39.0	35.0	41.0	32.0	41.0
32	36.692510148237375	39.0	35.0	41.0	31.0	41.0
33	36.52979396092172	39.0	35.0	41.0	30.0	41.0
34	36.38593884652609	39.0	35.0	41.0	30.0	41.0
35	36.19084792078296	39.0	35.0	41.0	30.0	41.0
36	36.04813095149466	39.0	35.0	41.0	29.0	41.0
37	35.95993461423308	39.0	35.0	41.0	29.0	41.0
38	35.89684495464291	39.0	35.0	41.0	28.0	41.0
39	35.83082182646313	39.0	35.0	41.0	27.0	41.0
40	35.7702601880055	39.0	35.0	41.0	27.0	41.0
41	35.81025380368095	39.0	35.0	41.0	28.0	41.0
42	35.641184356476685	39.0	35.0	40.0	27.0	41.0
43	35.66019646794742	39.0	35.0	40.0	27.0	41.0
44	35.43123916418908	38.0	35.0	40.0	26.0	41.0
45	35.3591921687047	38.0	35.0	40.0	26.0	41.0
46	35.385048147555025	38.0	35.0	40.0	26.0	41.0
47	35.264910906001	38.0	35.0	40.0	26.0	41.0
48	35.259782022449045	38.0	35.0	40.0	26.0	41.0
49	35.2553344191684	38.0	35.0	40.0	26.0	41.0
50	34.59585725892882	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	2.0
13	5.0
14	2.0
15	19.0
16	53.0
17	170.0
18	479.0
19	1124.0
20	2229.0
21	3833.0
22	6350.0
23	9724.0
24	14583.0
25	21890.0
26	28752.0
27	31839.0
28	31732.0
29	33234.0
30	39447.0
31	50532.0
32	66909.0
33	89112.0
34	136229.0
35	214476.0
36	185507.0
37	287050.0
38	327201.0
39	284587.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.69967108945392	23.69709165094241	29.714478223401013	14.888759036202655
2	32.86336420696995	25.103464085228588	29.685181029343795	12.347990678457672
3	26.723325761767214	25.28235371621784	35.10457277246257	12.889747749552374
4	24.30204925035068	27.252978324896777	34.351736648754496	14.093235775998044
5	22.027794306917833	32.00581873338643	32.67542297489172	13.290963984804021
6	19.36490967412629	40.169987997255596	30.161541621564876	10.303560707053233
7	85.51497450822758	3.347378490289346	9.548046594857299	1.589600406625772
8	43.79737696383591	50.79201509528551	4.31375741601962	1.0968505248589637
9	81.96915707098759	4.70319050192467	10.434032306181921	2.8936201209058243
10	42.076983599462906	32.33928185989514	15.933228106239017	9.65050643440294
11	35.62217438739674	22.597777376674614	28.299857584572212	13.480190651356427
12	31.92505060059248	22.504851176145767	29.169614685660388	16.400483537601367
13	20.910483950011596	34.27712788948263	30.010342391540128	14.80204576896565
14	16.121972734863608	36.56080935239275	31.408145262665144	15.909072650078492
15	15.315416162089	24.919164917493852	44.60768272049352	15.157736199923624
16	17.22251888383582	22.703236563326662	42.09851462690532	17.975729925932193
17	18.00941902110951	22.879983803525626	30.984701722964232	28.125895452400627
18	20.772513983116887	24.651366068707546	35.21126383381903	19.364856114356535
19	25.230936337250874	25.66600234698911	29.34754024079401	19.755521074966005
20	26.637094532458022	25.291298197767308	28.958000035349446	19.11360723442522
21	21.661713280626948	28.048287346022356	31.138311142628062	19.151688230722634
22	21.711523866501203	24.196804302777664	30.42409161291497	23.667580217806158
23	18.281824010094947	29.47265586294698	30.02539268684192	22.220127440116162
24	18.357343285452686	25.649238139055086	37.80409228776807	18.189326287724157
25	17.502957838284843	27.674118794498128	35.286515310327985	19.536408056889044
26	17.761330167593876	32.4057495341639	30.4486219874638	19.384298310778423
27	18.528627429136407	30.708119071937734	31.692226281457664	19.0710272174682
28	16.189083126369454	28.017490478411933	37.92647636166341	17.866950033555195
29	16.8708989953794	24.850287053586015	37.32548218521718	20.953331765817403
30	19.075579797897564	28.562568255231586	32.424120535190646	19.937731411680208
31	25.421609117586723	25.78635114963368	27.48296397623446	21.309075756545138
32	25.932515761301246	27.605669408748344	28.29953622595367	18.162278603996736
33	23.962640989398913	27.24917558124401	28.276023487030233	20.51215994232684
34	19.278678444817103	27.423887550192198	30.856693873244378	22.440740131746324
35	19.57470329226549	26.774957379813213	31.345480332049146	22.30485899587215
36	25.869583031836463	25.58935831646647	29.441644756257524	19.099413895439547
37	20.42432191992493	29.732902784197513	29.761343021938618	20.08143227393894
38	20.202209554741565	30.427626557718956	27.88085950576116	21.489304381778325
39	20.652004501163052	29.42257747822394	29.098433751652987	20.826984268960025
40	22.498584683084164	26.32805466095862	28.138964036221402	23.034396619735812
41	19.08511343691436	24.81831187104093	28.682595699257607	27.413978992787108
42	21.768029423595113	26.885076266434144	27.0374002516238	24.309494058346942
43	21.71136318719193	27.132201044094153	27.683491754205647	23.472944014508272
44	20.490521795344907	29.899955706070408	28.9240967010931	20.685425797491583
45	18.412242049453877	32.21604082968368	26.491947556415845	22.879769564446597
46	21.871614018305657	30.034069369542593	27.699559685132826	20.39475692701892
47	21.084338962643667	26.49816048970769	29.109306384913715	23.30819416273493
48	21.703597020577128	24.967100911426602	30.777211174924602	22.552090893071668
49	20.790135147367028	23.753115170108508	32.03945426879399	23.41729541373048
50	19.533087351164095	29.406027509368943	29.152154200719522	21.90873093874744
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	421.0
1	888.5
2	1356.0
3	12123.0
4	22890.0
5	16849.5
6	10809.0
7	11112.0
8	11415.0
9	11941.0
10	12467.0
11	12693.5
12	12920.0
13	12641.0
14	12362.0
15	11915.0
16	11468.0
17	11006.0
18	10544.0
19	10588.5
20	10633.0
21	11029.5
22	11426.0
23	12207.0
24	12988.0
25	14843.0
26	16698.0
27	19423.0
28	22148.0
29	26651.5
30	31155.0
31	36936.0
32	42717.0
33	47681.5
34	52646.0
35	59456.5
36	66267.0
37	76024.0
38	85781.0
39	103069.5
40	120358.0
41	140084.0
42	159810.0
43	171292.0
44	182774.0
45	188397.5
46	194021.0
47	186339.5
48	178658.0
49	166406.0
50	154154.0
51	136957.5
52	119761.0
53	104532.0
54	89303.0
55	78010.0
56	66717.0
57	58042.0
58	49367.0
59	42088.0
60	34809.0
61	29468.0
62	24127.0
63	19675.5
64	15224.0
65	11717.0
66	8210.0
67	6298.5
68	4387.0
69	3677.5
70	2968.0
71	2397.5
72	1827.0
73	1344.5
74	862.0
75	601.5
76	341.0
77	253.0
78	165.0
79	112.5
80	60.0
81	46.0
82	32.0
83	23.0
84	14.0
85	10.5
86	7.0
87	6.0
88	5.0
89	3.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1867073.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.37618603333513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.33548090717106	23.95116249887501
2	6.919383641889432	4.342077367678774
3	2.8791846840713666	2.7101350281525733
4	1.8419984252914827	2.311795410602237
5	1.3286900212758013	2.0844612644092777
6	1.0221303147781555	1.9242330544074482
7	0.8442021555684311	1.854149071800033
8	0.6960545637231055	1.7471629984582464
9	0.6271070148086185	1.7708603723500194
>10	7.150220075615445	47.9510419085698
>50	0.3164918921975952	6.258522211196336
>100	0.03693563050954406	1.743110060714614
>500	0.001413782066699361	0.30635495934102586
>1k	3.5344551667484026E-4	0.25351219440853756
>5k	3.5344551667484026E-4	0.7914215990361018
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7303	0.3911469985372827	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6970	0.3733115952081145	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3562	0.19077989987536642	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.355976975726177E-5	0.0	0.0	0.030743307840668255	0.0
2	5.355976975726177E-5	0.0	0.0	0.12752581179204026	0.0
3	5.355976975726177E-5	0.0	0.0	0.21402483995001803	0.0
4	5.355976975726177E-5	0.0	0.0	0.3384441850961371	0.0
5	5.355976975726177E-5	0.0	0.0	0.6161515912875394	0.0
6	5.355976975726177E-5	0.0	0.0	0.9838394106711414	0.0
7	5.355976975726177E-5	0.0	0.0	1.2415690227430851	0.0
8	5.355976975726177E-5	0.0	0.0	1.8335651578700993	0.0
9	5.355976975726177E-5	0.0	0.0	2.3934254311427567	0.0
10	5.355976975726177E-5	0.0	0.0	3.181450323581349	0.0
11	5.355976975726177E-5	0.0	0.0	3.6865189523923276	0.0
12	5.355976975726177E-5	0.0	0.0	4.22222376950446	0.0
13	5.355976975726177E-5	0.0	0.0	4.446907003636173	0.0
14	5.355976975726177E-5	0.0	0.0	4.557615047724433	0.0
15	5.355976975726177E-5	0.0	0.0	4.680266920468562	0.0
16	5.355976975726177E-5	0.0	0.0	4.912234283287263	0.0
17	5.355976975726177E-5	0.0	0.0	5.198564812409584	0.0
18	5.355976975726177E-5	0.0	0.0	5.5908901258815265	0.0
19	5.355976975726177E-5	0.0	0.0	5.812788251985863	0.0
20	5.355976975726177E-5	0.0	0.0	6.054878411288685	0.0
21	5.355976975726177E-5	0.0	0.0	6.318392478494413	0.0
22	5.355976975726177E-5	0.0	0.0	6.605740643242123	0.0
23	5.355976975726177E-5	0.0	0.0	6.919333095170891	0.0
24	5.355976975726177E-5	0.0	0.0	7.154996082102842	0.0
25	5.355976975726177E-5	0.0	0.0	7.351292638263207	0.0
26	5.355976975726177E-5	0.0	0.0	7.542875934684932	0.0
27	5.355976975726177E-5	0.0	0.0	7.738583333377966	0.0
28	5.355976975726177E-5	0.0	0.0	7.945591843489783	0.0
29	1.0711953951452354E-4	0.0	0.0	8.173649343116203	0.0
30	1.0711953951452354E-4	0.0	0.0	8.470906065269007	0.0
31	1.0711953951452354E-4	0.0	0.0	8.727939400334106	0.0
32	1.0711953951452354E-4	0.0	0.0	8.969493961939357	0.0
33	1.0711953951452354E-4	0.0	0.0	9.201729123606844	0.0
34	1.0711953951452354E-4	0.0	0.0	9.446818630016073	0.0
35	1.0711953951452354E-4	0.0	0.0	9.731328126966648	0.0
36	1.0711953951452354E-4	0.0	0.0	9.986647549399514	0.0
37	1.0711953951452354E-4	0.0	0.0	10.242234770681168	0.0
38	1.0711953951452354E-4	0.0	0.0	10.506820033282041	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGCTA	25	4.4439126E-5	44.0	13
TAAGTCG	25	4.4439126E-5	44.0	1
CGCGAGT	20	7.857746E-4	44.0	4
CCGGTCG	20	7.857746E-4	44.0	12
CACGTAT	30	2.52857E-6	44.0	40
TCGCGGA	55	1.8189894E-12	44.0	2
GACGGTC	340	0.0	43.352943	29
CGTTTTT	11790	0.0	43.10433	1
CGGTCTA	345	0.0	42.72464	31
CGACGGT	350	0.0	42.11429	28
ACGGTCT	355	0.0	41.521126	30
TCACGAC	365	0.0	40.9863	25
GAGCGAT	985	0.0	40.8731	7
TAGCACG	70	0.0	40.857143	1
TATAGCG	60	3.6379788E-12	40.333332	1
GCTTGCG	55	7.8216544E-11	40.0	1
GCGATCG	55	7.8216544E-11	40.0	9
GTTTTTA	6970	0.0	39.423244	2
GGTACGA	95	0.0	39.368423	2
TGCTACG	130	0.0	38.923077	1
>>END_MODULE