FastQCFastQC Report
Thu 26 May 2016
SRR1546469_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546469_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1776345
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138440.7793531098970077No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129050.7264917569503672No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGA207.8576413E-444.0000042
GTCGACG254.443825E-544.01
CGTTTTT193250.043.3624841
GTTTTTA103750.040.734462
ACGTGCG557.8216544E-1140.01
GACCGAT5800.039.8275879
GAGCGAT10000.039.827
TTAGCGA1550.039.7419322
CGAGGAT2750.039.26
TCACGAC2000.038.525
GTTAGCG1550.038.322581
CGAGATT4850.037.6494836
TTATTTT117050.036.6885956
TCTAGCG601.9826984E-1036.6666681
TCGAACG301.3013808E-436.6666681
TATACGA900.036.6666642
CGACCCG1750.036.4571432
GAGACCG4350.036.413797
TATTTTT118900.036.080747
CGACGGT2150.035.81395328
Produced by FastQC (version 0.11.5)