##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546469_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1776345
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.55504476889343	31.0	31.0	34.0	30.0	34.0
2	31.927117761470885	31.0	31.0	34.0	30.0	34.0
3	31.97945781928623	33.0	31.0	34.0	30.0	34.0
4	35.69477269336756	37.0	35.0	37.0	33.0	37.0
5	35.64571184088676	37.0	35.0	37.0	33.0	37.0
6	35.69022402742711	37.0	35.0	37.0	33.0	37.0
7	36.07825900937036	37.0	35.0	37.0	35.0	37.0
8	18.080986520073523	15.0	0.0	37.0	0.0	37.0
9	27.736574820769615	19.0	17.0	39.0	17.0	39.0
10	33.58059498577135	32.0	32.0	39.0	27.0	39.0
11	35.75991938502937	37.0	35.0	39.0	32.0	39.0
12	36.60281870920345	38.0	35.0	39.0	33.0	39.0
13	36.85887707624364	39.0	35.0	39.0	33.0	39.0
14	38.05259338698282	40.0	37.0	41.0	33.0	41.0
15	38.2563257700503	40.0	37.0	41.0	33.0	41.0
16	38.3091319535338	40.0	37.0	41.0	33.0	41.0
17	38.26840506770926	40.0	37.0	41.0	33.0	41.0
18	38.17757361323392	40.0	37.0	41.0	33.0	41.0
19	38.14864961479893	40.0	37.0	41.0	34.0	41.0
20	38.00890817943586	40.0	36.0	41.0	34.0	41.0
21	37.891416363375356	40.0	36.0	41.0	33.0	41.0
22	37.88271422499571	40.0	36.0	41.0	33.0	41.0
23	37.69210316689607	39.0	36.0	41.0	33.0	41.0
24	37.45502928766653	39.0	35.0	41.0	33.0	41.0
25	37.322462134326386	39.0	35.0	41.0	33.0	41.0
26	37.2783034827131	39.0	35.0	41.0	33.0	41.0
27	37.129194497690484	39.0	35.0	41.0	32.0	41.0
28	37.059440592902845	39.0	35.0	41.0	32.0	41.0
29	36.95669084552832	39.0	35.0	41.0	32.0	41.0
30	36.781940445127496	39.0	35.0	40.0	31.0	41.0
31	36.679737325801014	39.0	35.0	41.0	31.0	41.0
32	36.302857271532275	39.0	35.0	41.0	30.0	41.0
33	36.0152757487988	39.0	35.0	41.0	28.0	41.0
34	35.7412079297659	39.0	35.0	41.0	25.0	41.0
35	35.47473773394245	39.0	35.0	41.0	24.0	41.0
36	35.31974588269733	39.0	35.0	41.0	23.0	41.0
37	35.25878250002111	39.0	35.0	41.0	23.0	41.0
38	35.169999071126384	39.0	35.0	41.0	23.0	41.0
39	35.088946122515615	39.0	35.0	41.0	22.0	41.0
40	35.03695509599768	39.0	35.0	41.0	22.0	41.0
41	35.035548837641336	39.0	35.0	40.0	22.0	41.0
42	34.86446608063186	39.0	35.0	40.0	21.0	41.0
43	34.877997235897304	39.0	35.0	40.0	21.0	41.0
44	34.63696016258103	38.0	34.0	40.0	19.0	41.0
45	34.55832904081133	38.0	34.0	40.0	20.0	41.0
46	34.56204228345282	38.0	34.0	40.0	20.0	41.0
47	34.45049582147612	38.0	34.0	40.0	20.0	41.0
48	34.42232674396021	38.0	34.0	40.0	20.0	41.0
49	34.38417311952352	38.0	34.0	40.0	19.0	41.0
50	33.79683451131396	38.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	14.0
15	23.0
16	88.0
17	224.0
18	591.0
19	1379.0
20	2716.0
21	4585.0
22	7443.0
23	11472.0
24	17743.0
25	28001.0
26	37636.0
27	40936.0
28	38944.0
29	38119.0
30	40991.0
31	49694.0
32	62661.0
33	81893.0
34	117768.0
35	170038.0
36	176005.0
37	270505.0
38	300689.0
39	276183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.60092493293814	22.301298452721742	27.107234236592554	19.990542377747566
2	36.73233521641348	23.37518894133741	28.003625421863433	11.88885042038568
3	26.204650560561156	23.80010639825034	37.77216700584628	12.223076035342235
4	23.479785739819686	25.869805696528548	37.114749668561004	13.535658895090762
5	21.554089999408898	29.74540418668671	35.62269716749843	13.077808646405964
6	18.372613428134738	38.65848131978867	32.50201959641849	10.466885655658107
7	81.53072742063057	2.7866208422350387	13.835938401605544	1.8467133355288528
8	41.098716747028305	51.01137447961966	6.573216351553331	1.3166924217986935
9	77.01099730063699	4.723181589162015	15.247544818151878	3.0182762920491233
10	35.493499292085716	34.7259119146337	19.93571068683167	9.844878106448917
11	27.280398796404974	23.451018805468532	35.374209401889836	13.894372996236653
12	26.068978717535163	21.818453059512652	34.99984518773099	17.112723035221197
13	20.822925726702866	26.860041264506613	35.91712195547599	16.399911053314533
14	16.91597071514824	29.703295249515154	36.140445690448644	17.240288344887958
15	16.375929225460144	25.352338650431083	42.69654824935471	15.575183874754059
16	19.151009516732394	23.82487636129243	40.706563195775594	16.317550926199583
17	18.981560451376282	24.38878708809381	35.14221617985245	21.487436280677457
18	19.99662227776699	24.731513304003442	36.97890893942336	18.2929554788062
19	21.947256867331514	26.816919010665156	33.12740486786069	18.108419254142635
20	23.661619786696843	25.462564985968378	32.71813752396071	18.157677703374063
21	21.58049252819694	26.897871753516352	33.810718075599056	17.710917642687654
22	20.698344071675265	23.454846890665944	34.87835977808365	20.968449259575138
23	18.804905578589747	27.200966028558643	34.14471850907341	19.849409883778208
24	18.283441561183217	25.219312689820956	38.11213474859895	18.385111000396883
25	18.133358103296377	26.853398410781686	35.94391855185789	19.069324934064046
26	18.341031725256073	29.312098719561796	33.71574778548086	18.63112176970127
27	19.179551269601344	26.960641091679825	34.727150412785804	19.132657225933023
28	17.670666452744257	26.81112058749849	37.72189523994494	17.79631771981231
29	18.485992304422847	24.558686516414323	36.53288071855411	20.42244046060872
30	19.47808561962907	26.185960497538485	34.50427704077755	19.831676842054893
31	22.243483107166682	25.842052078847296	31.598591489828838	20.315873324157188
32	22.378704587228267	26.65484463885112	31.618069688039203	19.348381085881403
33	21.264844385521954	28.42702290377151	31.01418924814718	19.29394346255936
34	18.625435937275697	28.176958867787505	32.582859748528584	20.614745446408214
35	18.84487529168039	28.703939831507956	31.632143530676753	20.8190413461349
36	21.061899574688475	28.79029692993197	29.972781188338978	20.175022307040578
37	19.377823564679158	29.48318035066386	30.203310730742057	20.93568535391492
38	19.10963241937799	29.88985810751853	28.94876839803079	22.05174107507269
39	19.607452381153436	28.5499156976826	29.189543697874004	22.65308822328996
40	21.26754656330837	26.565166113564654	29.749288567254673	22.41799875587231
41	18.8894049297856	26.262465906116212	29.863342987989384	24.98478617610881
42	20.89954372602169	27.43447922560088	28.806735178132627	22.8592418702448
43	20.715683045804727	27.40830187829504	29.954147420686862	21.921867655213372
44	20.78329378583552	29.089056461441892	29.805921710028176	20.32172804269441
45	19.57587067827477	29.564133093515053	29.148054009778505	21.711942218431666
46	21.37614033309971	28.587408414469035	29.056179965040574	20.980271287390682
47	20.756553484824174	26.660192699053393	30.005713980110844	22.577539836011585
48	21.218963658523542	25.225505180581475	30.736540480593575	22.818990680301404
49	20.77513095710574	25.062417492097538	31.20266614874926	22.959785402047466
50	19.573787749564413	27.2166161415716	31.401670283644222	21.807925825219762
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	480.0
1	996.0
2	1512.0
3	22498.0
4	43484.0
5	30942.0
6	18400.0
7	18309.0
8	18218.0
9	18521.0
10	18824.0
11	18781.0
12	18738.0
13	18195.0
14	17652.0
15	16888.5
16	16125.0
17	15331.0
18	14537.0
19	13892.0
20	13247.0
21	13440.0
22	13633.0
23	14493.5
24	15354.0
25	17152.0
26	18950.0
27	22044.5
28	25139.0
29	29026.5
30	32914.0
31	37809.5
32	42705.0
33	48543.5
34	54382.0
35	60875.5
36	67369.0
37	74376.0
38	81383.0
39	93048.0
40	104713.0
41	116984.5
42	129256.0
43	136566.5
44	143877.0
45	150926.5
46	157976.0
47	159558.0
48	161140.0
49	149836.0
50	138532.0
51	122060.5
52	105589.0
53	92955.5
54	80322.0
55	71670.5
56	63019.0
57	56719.5
58	50420.0
59	45339.0
60	40258.0
61	34524.5
62	28791.0
63	23121.0
64	17451.0
65	13444.0
66	9437.0
67	7306.5
68	5176.0
69	4151.0
70	3126.0
71	2536.0
72	1946.0
73	1543.5
74	1141.0
75	853.5
76	566.0
77	445.0
78	324.0
79	224.0
80	124.0
81	92.5
82	61.0
83	43.0
84	25.0
85	21.5
86	18.0
87	11.5
88	5.0
89	3.0
90	1.0
91	2.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1776345.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.507815511295284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.37219911740489	31.1889190374067
2	8.992316810059602	7.644874879622658
3	4.1283590647601045	5.264625764676181
4	2.5988119629065576	4.418792778711164
5	1.8337835657795256	3.897506675090064
6	1.4855628138314345	3.7888818012472396
7	1.1753106783374594	3.4971922638256823
8	1.0025142769110462	3.4091753544299475
9	0.8122003836058325	3.107237765806798
>10	4.558898730105609	29.907415582560425
>50	0.027664955898384882	0.7775116155564299
>100	0.010385921681134322	0.9062279407837042
>500	0.0012803906046805352	0.3549219556005884
>1k	4.2679686822684514E-4	0.21909733261900355
>5k	0.0	0.0
>10k+	2.8453124548456337E-4	1.6176192520634076
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13844	0.7793531098970077	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12905	0.7264917569503672	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03068097694986053	0.0
2	0.0	0.0	0.0	0.10544122904052985	0.0
3	0.0	0.0	0.0	0.19607677562635636	0.0
4	0.0	0.0	0.0	0.31463482600508347	0.0
5	0.0	0.0	0.0	0.5723550323839119	0.0
6	0.0	0.0	0.0	1.0995611775865612	0.0
7	0.0	0.0	0.0	1.4877177575302094	0.0
8	1.6888611165060842E-4	0.0	0.0	2.257219177580932	0.0
9	1.6888611165060842E-4	0.0	0.0	3.15890212768353	0.0
10	1.6888611165060842E-4	0.0	0.0	4.076066304687434	0.0
11	1.6888611165060842E-4	0.0	0.0	4.521644162592289	0.0
12	1.6888611165060842E-4	0.0	0.0	4.966659066791642	0.0
13	1.6888611165060842E-4	0.0	0.0	5.238847183401873	0.0
14	1.6888611165060842E-4	0.0	0.0	5.39078838851687	0.0
15	1.6888611165060842E-4	0.0	0.0	5.5165522463260235	0.0
16	1.6888611165060842E-4	0.0	0.0	5.68493169964168	0.0
17	1.6888611165060842E-4	0.0	0.0	5.869524219675795	0.0
18	1.6888611165060842E-4	0.0	0.0	6.176559170656601	0.0
19	1.6888611165060842E-4	0.0	0.0	6.3475845063881176	0.0
20	1.6888611165060842E-4	0.0	0.0	6.536399179213498	0.0
21	1.6888611165060842E-4	0.0	0.0	6.718120635349552	0.0
22	1.6888611165060842E-4	0.0	0.0	6.909862667443543	0.0
23	2.81476852751014E-4	0.0	0.0	7.122208805158908	0.0
24	3.9406759385141965E-4	0.0	0.0	7.297174816828938	0.0
25	3.9406759385141965E-4	0.0	0.0	7.4469767978630275	0.0
26	3.9406759385141965E-4	0.0	0.0	7.596609892785467	0.0
27	3.9406759385141965E-4	0.0	0.0	7.76408862017232	0.0
28	3.9406759385141965E-4	0.0	0.0	7.937647247578595	0.0
29	3.9406759385141965E-4	0.0	0.0	8.117398365745393	0.0
30	3.9406759385141965E-4	0.0	0.0	8.354289285020647	0.0
31	3.9406759385141965E-4	0.0	0.0	8.553518601397814	0.0
32	3.9406759385141965E-4	0.0	0.0	8.7579270918656	0.0
33	3.9406759385141965E-4	0.0	0.0	8.96025265362303	0.0
34	3.9406759385141965E-4	0.0	0.0	9.170234385775286	0.0
35	3.9406759385141965E-4	0.0	0.0	9.393051462412988	0.0
36	3.9406759385141965E-4	0.0	0.0	9.60725534735651	0.0
37	3.9406759385141965E-4	0.0	0.0	9.824668068421394	0.0
38	3.9406759385141965E-4	0.0	0.0	10.05457836174842	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGCGA	20	7.8576413E-4	44.000004	2
GTCGACG	25	4.443825E-5	44.0	1
CGTTTTT	19325	0.0	43.362484	1
GTTTTTA	10375	0.0	40.73446	2
ACGTGCG	55	7.8216544E-11	40.0	1
GACCGAT	580	0.0	39.827587	9
GAGCGAT	1000	0.0	39.82	7
TTAGCGA	155	0.0	39.741932	2
CGAGGAT	275	0.0	39.2	6
TCACGAC	200	0.0	38.5	25
GTTAGCG	155	0.0	38.32258	1
CGAGATT	485	0.0	37.649483	6
TTATTTT	11705	0.0	36.688595	6
TCTAGCG	60	1.9826984E-10	36.666668	1
TCGAACG	30	1.3013808E-4	36.666668	1
TATACGA	90	0.0	36.666664	2
CGACCCG	175	0.0	36.45714	32
GAGACCG	435	0.0	36.41379	7
TATTTTT	11890	0.0	36.08074	7
CGACGGT	215	0.0	35.813953	28
>>END_MODULE