FastQCFastQC Report
Thu 26 May 2016
SRR1546468_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546468_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1308117
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109200.8347877139430188No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103410.7905256181213148No Hit
CGGTCGGAGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC19300.14754031940568008No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGA1100.044.02
CGATCGA351.4462967E-743.99999610
CGTTTTT124600.043.1171761
GTTTTTA67850.040.4008872
GTATGCG603.6379788E-1240.3333321
TATAGCG950.039.368421
TCGATAG900.039.1111071
CGGTCTA1850.038.05405431
GAGCGAT7950.037.635227
GCGCGAC6950.037.3525169
GCGATGT1500.036.6666689
TATGCGA900.036.6666642
TTATTTT76450.036.6330956
AGTCCCC3550.036.5633778
TATTTTT76950.036.480837
GCGTAAG1150.036.3478281
CGACCCG6600.036.33333232
ACCCGCT6600.036.33333234
TTAGCGA1700.036.2352942
CCGGTAT850.036.23529442
Produced by FastQC (version 0.11.5)