##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546468_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1308117 Sequences flagged as poor quality 0 Sequence length 50 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.576473664052987 31.0 31.0 34.0 30.0 34.0 2 31.965217178585707 31.0 31.0 34.0 30.0 34.0 3 32.025304311464495 33.0 31.0 34.0 30.0 34.0 4 35.72873374476442 37.0 35.0 37.0 35.0 37.0 5 35.69673890026657 37.0 35.0 37.0 33.0 37.0 6 35.7350344044149 37.0 35.0 37.0 35.0 37.0 7 36.05547133780847 37.0 35.0 37.0 35.0 37.0 8 19.027463139765022 33.0 0.0 37.0 0.0 37.0 9 28.256139932437236 33.0 17.0 39.0 17.0 39.0 10 33.86161788280406 34.0 32.0 39.0 27.0 39.0 11 35.957388368165844 37.0 35.0 39.0 32.0 39.0 12 36.64917816984261 38.0 35.0 39.0 33.0 39.0 13 36.81315050565049 39.0 35.0 39.0 33.0 39.0 14 37.95986826866404 40.0 37.0 41.0 33.0 41.0 15 38.19733708834913 40.0 37.0 41.0 33.0 41.0 16 38.271486419028264 40.0 37.0 41.0 33.0 41.0 17 38.23974690337332 40.0 37.0 41.0 33.0 41.0 18 38.17492242666367 40.0 37.0 41.0 33.0 41.0 19 38.12767971060693 40.0 37.0 41.0 34.0 41.0 20 37.971092035345464 40.0 36.0 41.0 34.0 41.0 21 37.86688652467631 40.0 36.0 41.0 33.0 41.0 22 37.858275674117834 40.0 36.0 41.0 33.0 41.0 23 37.68843077492304 39.0 35.0 41.0 33.0 41.0 24 37.44942998218049 39.0 35.0 41.0 33.0 41.0 25 37.31590369974551 39.0 35.0 41.0 33.0 41.0 26 37.271896168309105 39.0 35.0 41.0 33.0 41.0 27 37.16201761769016 39.0 35.0 41.0 33.0 41.0 28 37.105946180655096 39.0 35.0 41.0 32.0 41.0 29 36.96592047959013 39.0 35.0 41.0 32.0 41.0 30 36.81652405709887 39.0 35.0 40.0 31.0 41.0 31 36.743478603213624 39.0 35.0 41.0 31.0 41.0 32 36.41260758785338 39.0 35.0 41.0 30.0 41.0 33 36.160744031306066 39.0 35.0 41.0 30.0 41.0 34 35.87643536472655 39.0 35.0 41.0 27.0 41.0 35 35.624754513548865 39.0 35.0 41.0 25.0 41.0 36 35.4420804866843 39.0 35.0 41.0 24.0 41.0 37 35.37533875027998 39.0 35.0 41.0 24.0 41.0 38 35.27006605678238 39.0 35.0 41.0 24.0 41.0 39 35.19255387706146 39.0 35.0 40.0 23.0 41.0 40 35.0906241567077 38.0 35.0 40.0 23.0 41.0 41 35.04722742690448 38.0 35.0 40.0 23.0 41.0 42 34.897836355616505 38.0 34.0 40.0 23.0 41.0 43 34.89222982347909 38.0 34.0 40.0 23.0 41.0 44 34.638215847665 38.0 34.0 40.0 22.0 41.0 45 34.547428861485635 38.0 34.0 40.0 22.0 41.0 46 34.54320752654388 38.0 34.0 40.0 23.0 41.0 47 34.42852435982408 38.0 34.0 40.0 22.0 41.0 48 34.40126838807232 37.0 34.0 40.0 22.0 41.0 49 34.36017344014335 37.0 34.0 40.0 22.0 41.0 50 33.78014810601804 37.0 33.0 40.0 20.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 3.0 13 2.0 14 12.0 15 27.0 16 67.0 17 152.0 18 395.0 19 849.0 20 1687.0 21 2996.0 22 5067.0 23 7546.0 24 11662.0 25 18207.0 26 24931.0 27 27576.0 28 27189.0 29 26891.0 30 29658.0 31 36533.0 32 46637.0 33 62022.0 34 93107.0 35 137766.0 36 135818.0 37 201091.0 38 217590.0 39 192634.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.70084709548152 22.058959557898874 26.886585832918612 20.353607513700993 2 37.28022799183865 23.234542475940607 27.037107537016947 12.448121995203792 3 27.55174040242578 23.324824920095068 36.33818687472145 12.785247802757704 4 25.316695677833103 25.338024045249774 35.18737238335714 14.15790789355998 5 21.49975881362294 29.71056870295241 34.03426451915234 14.75540796427231 6 19.396200798552425 38.845531401243164 31.313559872702516 10.444707927501899 7 81.7299217118958 3.406040896953407 12.92070969187007 1.9433276992807218 8 43.65289954950513 48.58410983115425 6.298977843724988 1.4640127756156367 9 77.82904740172324 4.712498958426502 14.160124820639133 3.2983288192111258 10 39.637815271875525 29.825084453454853 19.136361655723455 11.400738618946164 11 32.55901421661824 21.790634935560046 29.98225693879064 15.668093909031072 12 29.992577116572903 19.529522206346986 32.55091096591513 17.926989711164982 13 23.001000675016073 25.176723488801077 34.18891429436358 17.63336154181927 14 18.27229521518335 28.554173671009554 34.75866455370582 18.414866560101277 15 16.806524187056663 22.26773293214598 42.245074408481806 18.68066847231555 16 20.39205973166009 20.494191268823812 40.01522799566094 19.098521003855158 17 19.957771361430208 21.15751114005857 33.510458162381504 25.374259336129718 18 22.252902454444058 22.494241723026303 34.70247691911351 20.55037890341613 19 24.405767985585385 24.804585522548823 30.93805829295086 19.85158819891493 20 25.280231049669105 24.24393230880724 30.437644339153145 20.038192302370504 21 23.615165921702722 24.608960819253937 32.369046499663256 19.406826759380085 22 22.159332842551546 21.727337845162168 32.79783077507593 23.31549853721036 23 20.617880510688263 25.687610511903753 31.719104636664763 21.97540434074322 24 20.408495570350357 24.758641619977418 35.11528403040401 19.717578779268216 25 21.305663025555052 23.881961628814548 33.304436835543 21.507938510087403 26 19.29697420032 26.984818636253483 31.53724017041289 22.18096699301362 27 20.54112896629277 24.102736987593616 33.907441001072534 21.44869304504108 28 19.454299577178492 24.560647098080675 36.49658249223884 19.488470832501985 29 20.347644744315684 23.662944522546532 35.21619243538613 20.773218297751654 30 21.99023481844514 23.66225651069438 34.33775419171221 20.009754479148274 31 25.728738331510105 22.657376977747404 30.12054732107296 21.493337369669533 32 25.397575293341497 24.08691271499415 30.774464363661657 19.741047628002693 33 24.969861258587724 23.61218453701007 30.132855088650327 21.28509911575188 34 21.1790688447593 24.420980692094055 32.39473227547689 22.005218187669755 35 22.060106244319126 24.067342600088523 32.05561887812787 21.816932277464478 36 25.77636404083121 24.122918668590042 28.898867608937124 21.201849681641626 37 22.845815779475384 26.64906885240387 29.138448625008312 21.36666674311243 38 22.19648548256769 27.206740681452807 27.35053515855233 23.246238677427172 39 22.446310230659797 26.255067398405497 27.658764468315905 23.639857902618804 40 22.92570160008623 24.41731129554925 29.384603976555614 23.272383127808904 41 20.774594321455954 23.684502227247258 29.8260782483524 25.714825202944386 42 22.10528568927703 26.175028686271943 27.58698189840817 24.132703726042852 43 21.889937979553817 25.735847787315663 28.660280387763482 23.713933845367045 44 22.404341507678595 26.22884650226241 29.97805242191639 21.388759568142603 45 21.0557618317016 27.00614700367016 28.66937743336414 23.2687137312641 46 23.467549156535693 25.477384668190993 29.40791993376739 21.647146241505922 47 22.166671635641155 25.21899799482768 29.986920130232996 22.627410239298168 48 22.370399589639153 24.346063845970964 30.353554001668048 22.929982562721836 49 22.3272841802377 23.501796857620533 30.51194961918544 23.658969342956325 50 20.430129720812435 25.304999476346534 31.212039901629595 23.052830901211436 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 430.0 1 1067.5 2 1705.0 3 17472.0 4 33239.0 5 22725.0 6 12211.0 7 12208.5 8 12206.0 9 12238.0 10 12270.0 11 12089.5 12 11909.0 13 11517.0 14 11125.0 15 10530.0 16 9935.0 17 9179.5 18 8424.0 19 7991.5 20 7559.0 21 7425.0 22 7291.0 23 7790.0 24 8289.0 25 9338.5 26 10388.0 27 11850.0 28 13312.0 29 15128.0 30 16944.0 31 19164.0 32 21384.0 33 24421.0 34 27458.0 35 31162.5 36 34867.0 37 39200.5 38 43534.0 39 51029.0 40 58524.0 41 67018.5 42 75513.0 43 80866.0 44 86219.0 45 92250.5 46 98282.0 47 103562.0 48 108842.0 49 107005.5 50 105169.0 51 94819.5 52 84470.0 53 77599.0 54 70728.0 55 67685.0 56 64642.0 57 62013.0 58 59384.0 59 55921.5 60 52459.0 61 48857.0 62 45255.0 63 38964.5 64 32674.0 65 27141.0 66 21608.0 67 18149.5 68 14691.0 69 12610.5 70 10530.0 71 8808.0 72 7086.0 73 5603.5 74 4121.0 75 2967.5 76 1814.0 77 1370.5 78 927.0 79 675.0 80 423.0 81 299.5 82 176.0 83 127.0 84 78.0 85 44.5 86 11.0 87 7.5 88 4.0 89 3.5 90 3.0 91 3.0 92 3.0 93 1.5 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1308117.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.52628693526255 #Duplication Level Percentage of deduplicated Percentage of total 1 70.0337028989136 25.580711272246464 2 7.892338154024214 5.765556160080177 3 4.192354458135043 4.593934256165031 4 3.0118224681408545 4.4004276667753395 5 2.354130000668238 4.299381394365893 6 1.8934147202077385 4.1495645614654615 7 1.6511436423143115 4.221710251535494 8 1.4030052386152487 4.0997257533869655 9 1.1961056039590638 3.932036684557584 >10 6.317779439184816 34.3285872525847 >50 0.026056584002165158 0.6321592052195888 >100 0.026453753395755886 1.886597329541004 >500 6.348894146146443E-4 0.13813601095526928 >1k 6.348894146146443E-4 0.32798606294527566 >5k 0.0 0.0 >10k+ 4.2325960974309623E-4 1.6434861381757024 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10920 0.8347877139430188 No Hit CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10341 0.7905256181213148 No Hit CGGTCGGAGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC 1930 0.14754031940568008 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.029278726597085733 0.0 2 0.0 0.0 0.0 0.0856956984734546 0.0 3 0.0 0.0 0.0 0.17406699859416244 0.0 4 0.0 0.0 0.0 0.2440913159908479 0.0 5 0.0 0.0 0.0 0.4141066892334554 0.0 6 0.0 0.0 0.0 0.7698088167954396 0.0 7 0.0 0.0 0.0 1.0340053680213621 0.0 8 0.0 0.0 0.0 1.66315398393263 0.0 9 0.0 0.0 0.0 2.370506613705043 7.64457613500933E-5 10 0.0 0.0 0.0 3.1851126466516373 7.64457613500933E-5 11 0.0 0.0 0.0 3.669014315997728 7.64457613500933E-5 12 0.0 0.0 0.0 4.068214081767915 7.64457613500933E-5 13 0.0 0.0 0.0 4.327288766983381 7.64457613500933E-5 14 0.0 0.0 0.0 4.461680415436845 7.64457613500933E-5 15 0.0 0.0 0.0 4.575049479519034 7.64457613500933E-5 16 0.0 0.0 0.0 4.709746911017898 7.64457613500933E-5 17 0.0 0.0 0.0 4.853694279640124 7.64457613500933E-5 18 0.0 0.0 0.0 5.081502648463402 7.64457613500933E-5 19 0.0 0.0 0.0 5.197929542999594 7.64457613500933E-5 20 0.0 0.0 0.0 5.344399621746373 7.64457613500933E-5 21 0.0 0.0 0.0 5.48995235135695 7.64457613500933E-5 22 0.0 0.0 0.0 5.650641341714847 7.64457613500933E-5 23 0.0 0.0 0.0 5.818898462446402 7.64457613500933E-5 24 0.0 0.0 0.0 5.955201254933618 7.64457613500933E-5 25 0.0 0.0 0.0 6.07094013761766 7.64457613500933E-5 26 0.0 0.0 0.0 6.183926972893097 7.64457613500933E-5 27 0.0 0.0 0.0 6.326651209333722 7.64457613500933E-5 28 0.0 0.0 0.0 6.453321835890827 7.64457613500933E-5 29 0.0 0.0 0.0 6.594440711343099 7.64457613500933E-5 30 0.0 0.0 0.0 6.795263726409794 7.64457613500933E-5 31 0.0 0.0 0.0 6.944791635610576 7.64457613500933E-5 32 0.0 0.0 0.0 7.106703758150074 7.64457613500933E-5 33 0.0 0.0 0.0 7.261582870645363 7.64457613500933E-5 34 0.0 0.0 0.0 7.408282286676192 7.64457613500933E-5 35 0.0 0.0 0.0 7.585712898769758 7.64457613500933E-5 36 0.0 0.0 0.0 7.738680867231295 7.64457613500933E-5 37 0.0 0.0 0.0 7.905103289690448 7.64457613500933E-5 38 0.0 0.0 0.0 8.109748592824648 7.64457613500933E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAAGA 110 0.0 44.0 2 CGATCGA 35 1.4462967E-7 43.999996 10 CGTTTTT 12460 0.0 43.117176 1 GTTTTTA 6785 0.0 40.400887 2 GTATGCG 60 3.6379788E-12 40.333332 1 TATAGCG 95 0.0 39.36842 1 TCGATAG 90 0.0 39.111107 1 CGGTCTA 185 0.0 38.054054 31 GAGCGAT 795 0.0 37.63522 7 GCGCGAC 695 0.0 37.352516 9 GCGATGT 150 0.0 36.666668 9 TATGCGA 90 0.0 36.666664 2 TTATTTT 7645 0.0 36.633095 6 AGTCCCC 355 0.0 36.563377 8 TATTTTT 7695 0.0 36.48083 7 GCGTAAG 115 0.0 36.347828 1 CGACCCG 660 0.0 36.333332 32 ACCCGCT 660 0.0 36.333332 34 TTAGCGA 170 0.0 36.235294 2 CCGGTAT 85 0.0 36.235294 42 >>END_MODULE