FastQCFastQC Report
Thu 26 May 2016
SRR1546467_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546467_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1475137
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51610.34986580907400466No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48900.331494634057718No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCC19500.1321911117408078No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA19080.12934391856485195No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATG14890.10093977711900658No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATCG207.8572077E-444.00000420
CATCGAA207.8572077E-444.00000440
TCGGCAT502.7284841E-1144.032
CGACGGT1950.042.87179628
CGGTCTA1950.042.87179631
CGTTTTT71100.042.3909951
GCGCGAC1900.041.684219
CGACCCG1650.041.33333232
CGCAAGA800.041.2500042
TGCGCGA700.040.8571432
CACGACG2050.040.78048326
GAGCGAT9400.040.7234087
CGCGCGA603.6379788E-1240.3333362
GCCGATT603.6379788E-1240.3333369
ACCGCGA501.3478711E-939.62
TTTCGCG452.3517714E-839.111111
CCAGTCG452.3517714E-839.1111128
GACGGTC2150.038.88372429
CGATGAA7050.038.69503419
GCGATAC1200.038.5000049