##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546467_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1475137
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.595716194495832	31.0	31.0	34.0	30.0	34.0
2	31.96717660800319	31.0	31.0	34.0	30.0	34.0
3	32.05969208283705	33.0	31.0	34.0	30.0	34.0
4	35.74663709201247	37.0	35.0	37.0	35.0	37.0
5	35.68609898606028	37.0	35.0	37.0	33.0	37.0
6	35.73293328009534	37.0	35.0	37.0	33.0	37.0
7	36.09918739750952	37.0	35.0	37.0	35.0	37.0
8	18.19407417751707	25.0	0.0	37.0	0.0	37.0
9	27.836842950858124	23.0	17.0	39.0	17.0	39.0
10	33.66854468432424	32.0	32.0	39.0	27.0	39.0
11	35.791948815601536	37.0	35.0	39.0	32.0	39.0
12	36.493547378989206	37.0	35.0	39.0	33.0	39.0
13	36.63393298385167	39.0	35.0	39.0	33.0	39.0
14	37.79492277666413	39.0	36.0	41.0	33.0	41.0
15	38.06469365218281	40.0	36.0	41.0	33.0	41.0
16	38.156032965073756	40.0	36.0	41.0	33.0	41.0
17	38.11831579032998	40.0	36.0	41.0	33.0	41.0
18	38.09365231839483	40.0	36.0	41.0	33.0	41.0
19	38.10302297345942	40.0	37.0	41.0	34.0	41.0
20	37.98434314914479	40.0	36.0	41.0	34.0	41.0
21	37.87668467403367	40.0	36.0	41.0	33.0	41.0
22	37.87622912312551	40.0	36.0	41.0	33.0	41.0
23	37.730957192450596	39.0	36.0	41.0	33.0	41.0
24	37.62303704672854	39.0	35.0	41.0	33.0	41.0
25	37.526513130644815	39.0	35.0	41.0	33.0	41.0
26	37.47590359403906	39.0	35.0	41.0	33.0	41.0
27	37.31007018331179	39.0	35.0	41.0	33.0	41.0
28	37.33988639699228	39.0	35.0	41.0	33.0	41.0
29	37.27118023614078	39.0	35.0	41.0	33.0	41.0
30	37.19304444265177	39.0	35.0	41.0	32.0	41.0
31	37.185442436871966	39.0	35.0	41.0	32.0	41.0
32	36.95134621394487	39.0	35.0	41.0	31.0	41.0
33	36.83185900699392	39.0	35.0	41.0	31.0	41.0
34	36.72184414057813	39.0	35.0	41.0	31.0	41.0
35	36.56685718004497	39.0	35.0	41.0	30.0	41.0
36	36.43943240526134	39.0	35.0	41.0	30.0	41.0
37	36.3573417248703	39.0	35.0	41.0	30.0	41.0
38	36.320587172581256	39.0	35.0	41.0	30.0	41.0
39	36.254602792825345	39.0	35.0	41.0	30.0	41.0
40	36.20631575236741	39.0	35.0	41.0	30.0	41.0
41	36.24167314629082	39.0	35.0	41.0	30.0	41.0
42	36.1036927417589	39.0	35.0	41.0	29.0	41.0
43	36.11294544167762	39.0	35.0	41.0	30.0	41.0
44	35.908712207747485	39.0	35.0	40.0	29.0	41.0
45	35.840220942190456	39.0	35.0	40.0	28.0	41.0
46	35.834481814231495	39.0	35.0	40.0	28.0	41.0
47	35.72374565887779	38.0	35.0	40.0	28.0	41.0
48	35.7431031829586	38.0	35.0	40.0	28.0	41.0
49	35.73088126729924	38.0	35.0	40.0	28.0	41.0
50	35.11442530422598	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	2.0
13	1.0
14	3.0
15	16.0
16	36.0
17	101.0
18	265.0
19	712.0
20	1398.0
21	2533.0
22	4231.0
23	6627.0
24	9790.0
25	14430.0
26	18685.0
27	20889.0
28	21820.0
29	24218.0
30	29531.0
31	38583.0
32	51283.0
33	70061.0
34	106632.0
35	169000.0
36	148251.0
37	231946.0
38	268746.0
39	235343.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.04787080793174	24.298692257058157	29.8359406617826	13.817496273227503
2	31.43497858165038	25.682767092141273	30.833881869955132	12.048372456253215
3	27.629433740730526	25.72649184448631	33.92423890120037	12.719835513582806
4	24.388988954924187	27.0761292002031	34.2276683453808	14.307213499491912
5	23.396470971848714	31.67549861470494	31.4560613692152	13.471969044231146
6	19.84080122727584	41.22545905905689	28.40509051023735	10.528649203429918
7	87.01754481109212	2.990501899145639	8.34729248876545	1.644660800996789
8	44.43058509141863	51.01512605269883	3.563330050022472	0.9909588058600659
9	83.96142188827207	4.3162092741216584	8.481449519603942	3.2409193180023275
10	43.91910717445227	33.79970809490915	13.112138059041294	9.169046671597282
11	35.262148532644765	22.672809372959936	28.719027453043346	13.346014641351955
12	32.2853402768692	22.770834166589275	28.118744225112653	16.825081331428876
13	22.079169595773138	33.32314218950511	29.208880259935178	15.388807954786573
14	16.918089641843437	36.209992698983214	30.298880714130284	16.57303694504307
15	15.580518961967602	24.998423875206164	43.2682523724915	16.15280479033473
16	18.14021341746563	23.050198049401512	40.508915443107995	18.30067309002486
17	18.64525125462923	22.840929350968757	30.612071963485427	27.901747430916586
18	21.238569705729027	24.35326345959731	34.080495574309374	20.32767126036429
19	24.75098923015286	26.000703663456342	28.609207144827902	20.63909996156289
20	27.27705969004913	25.646363693677266	27.96214860043508	19.114428015838527
21	22.0707635968727	28.134539368207832	30.647458507243737	19.147238527675732
22	22.03158079554645	23.65448090584129	30.292305053700098	24.021633244912167
23	19.21279176103643	28.61761314372834	30.186009841797745	21.983585253437475
24	18.881500497919852	26.029921288666745	36.67686458952626	18.411713623887138
25	17.76438391823946	28.063901861318648	34.23241366734073	19.93930055310117
26	18.044290123561403	32.637375375982025	29.405133218134992	19.913201282321573
27	18.762121755470847	30.31135413185352	31.567034112763764	19.359489999911872
28	16.05078036819631	28.15921504239945	37.16129417132103	18.6287104180832
29	17.332627410199866	24.898839904361424	36.469155068308915	21.2993776171298
30	19.15300070434136	29.1577663633954	32.50186253886927	19.18737039339397
31	25.274262661705322	26.40730996510833	27.373593096776773	20.94483427640958
32	26.22007311863237	27.470668826014126	27.88066464335177	18.42859341200173
33	24.170500773826433	27.561372265762436	28.47769393622423	19.790433024186907
34	19.250008643264998	27.08189137686873	30.809816308586928	22.858283671279345
35	21.024352314395205	26.38622717754351	31.063623243129285	21.525797264932002
36	25.19501578497455	25.568472623220757	29.26901026819882	19.967501323605873
37	20.40488442768367	29.084417243957677	30.587328498980092	19.923369829378558
38	20.93344550370576	29.910848958435725	27.159918027952656	21.99578750990586
39	20.766749122284914	28.74478777225437	29.215252549424225	21.27321055603649
40	22.755310184748943	26.08971234536182	27.849955631239677	23.305021838649562
41	18.92102225081467	25.11427752134209	28.791902040285073	27.172798187558172
42	21.912269843411156	26.945293894736555	26.645186175928064	24.49725008592422
43	21.429196067890643	26.83682939279538	27.84880319590655	23.88517134340743
44	20.822472760157194	29.091060694701575	28.60771575792621	21.478750787215017
45	19.41101063833393	31.28461966583443	26.619629227658177	22.684740468173466
46	22.210750594690527	29.584506388220213	28.04051420308758	20.16422881400168
47	21.240061092630718	26.629458823146596	29.16759595888382	22.962884125338867
48	22.03497031123211	24.574802204812162	30.55139963271208	22.83882785124365
49	21.20948766114605	23.703900044538237	31.687158548663614	23.3994537456521
50	19.59431564661452	28.778750719424707	29.64409407397415	21.98283955998663
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	275.0
1	731.0
2	1187.0
3	8701.0
4	16215.0
5	11410.0
6	6605.0
7	6745.0
8	6885.0
9	7235.5
10	7586.0
11	7543.0
12	7500.0
13	7257.0
14	7014.0
15	6778.5
16	6543.0
17	6391.0
18	6239.0
19	6324.0
20	6409.0
21	7092.5
22	7776.0
23	8616.0
24	9456.0
25	11387.5
26	13319.0
27	15960.5
28	18602.0
29	22029.5
30	25457.0
31	29926.5
32	34396.0
33	38586.0
34	42776.0
35	47903.5
36	53031.0
37	61280.0
38	69529.0
39	83707.5
40	97886.0
41	113294.5
42	128703.0
43	136005.0
44	143307.0
45	147069.5
46	150832.0
47	146205.0
48	141578.0
49	132339.0
50	123100.0
51	109000.5
52	94901.0
53	83408.5
54	71916.0
55	63261.5
56	54607.0
57	48106.5
58	41606.0
59	36034.0
60	30462.0
61	26107.5
62	21753.0
63	17455.5
64	13158.0
65	9893.5
66	6629.0
67	5056.5
68	3484.0
69	2801.5
70	2119.0
71	1611.5
72	1104.0
73	800.5
74	497.0
75	376.0
76	255.0
77	224.0
78	193.0
79	161.0
80	129.0
81	99.5
82	70.0
83	54.5
84	39.0
85	23.0
86	7.0
87	3.5
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1475137.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.52389465011339
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.52846458492591	22.44375106774872
2	6.92995999970564	4.230587379210296
3	3.074030237323969	2.814941253460197
4	2.0374330442873125	2.487615664019429
5	1.5658047984515449	2.3897230355288497
6	1.283928323417041	2.3514295729367083
7	1.067685285433884	2.2812939220442088
8	0.9179076633111506	2.241449345075305
9	0.8184574604265549	2.248425836789356
>10	8.6052271227554	51.180961732468845
>50	0.15011868521235094	2.9781828305444358
>100	0.018527362791641112	1.0088186158787755
>500	0.0011161054352617006	0.22178192955549964
>1k	0.0011161054352617006	0.769389108614655
>5k	2.2322108705234015E-4	0.35164870612471083
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5161	0.34986580907400466	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4890	0.331494634057718	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCC	1950	0.1321911117408078	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1908	0.12934391856485195	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATG	1489	0.10093977711900658	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03742025316970559	0.0
2	0.0	0.0	0.0	0.14283419099378566	0.0
3	6.779031371323477E-5	0.0	0.0	0.2254027930965056	0.0
4	6.779031371323477E-5	0.0	0.0	0.3634238718166516	0.0
5	6.779031371323477E-5	0.0	0.0	0.7039346175982298	0.0
6	6.779031371323477E-5	0.0	0.0	1.031971945656573	0.0
7	6.779031371323477E-5	0.0	0.0	1.2525616264794388	0.0
8	6.779031371323477E-5	0.0	0.0	1.7186878235716412	0.0
9	6.779031371323477E-5	0.0	0.0	2.112820707500388	0.0
10	6.779031371323477E-5	0.0	0.0	2.80862048745303	0.0
11	6.779031371323477E-5	0.0	0.0	3.3032864066185037	0.0
12	6.779031371323477E-5	0.0	0.0	3.8246617093869926	0.0
13	6.779031371323477E-5	0.0	0.0	4.013322152450925	0.0
14	6.779031371323477E-5	0.0	0.0	4.097924463965042	0.0
15	6.779031371323477E-5	0.0	0.0	4.218930173943166	0.0
16	6.779031371323477E-5	0.0	0.0	4.448468176176179	0.0
17	6.779031371323477E-5	0.0	0.0	4.742068024868199	0.0
18	6.779031371323477E-5	0.0	0.0	5.125422248916541	0.0
19	6.779031371323477E-5	0.0	0.0	5.360925798756319	0.0
20	6.779031371323477E-5	0.0	0.0	5.58497278557856	0.0
21	6.779031371323477E-5	0.0	0.0	5.8393220426306165	0.0
22	6.779031371323477E-5	0.0	0.0	6.126007279323886	0.0
23	6.779031371323477E-5	0.0	0.0	6.436351335503075	0.0
24	6.779031371323477E-5	0.0	0.0	6.670973611264581	0.0
25	6.779031371323477E-5	0.0	0.0	6.864243795661014	0.0
26	6.779031371323477E-5	0.0	0.0	7.061581398880239	0.0
27	6.779031371323477E-5	0.0	0.0	7.245632100611672	0.0
28	6.779031371323477E-5	0.0	0.0	7.456256605318693	0.0
29	6.779031371323477E-5	0.0	0.0	7.681794979042625	0.0
30	6.779031371323477E-5	0.0	0.0	7.969632651069019	0.0
31	6.779031371323477E-5	0.0	0.0	8.215169167338356	0.0
32	6.779031371323477E-5	0.0	0.0	8.450130394668427	0.0
33	6.779031371323477E-5	0.0	0.0	8.680414090352286	0.0
34	6.779031371323477E-5	0.0	0.0	8.921747607171401	0.0
35	6.779031371323477E-5	0.0	0.0	9.202602876885333	0.0
36	6.779031371323477E-5	0.0	0.0	9.452816924800883	0.0
37	6.779031371323477E-5	0.0	0.0	9.709742213774042	0.0
38	6.779031371323477E-5	0.0	0.0	9.971955147216836	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCATCG	20	7.8572077E-4	44.000004	20
CATCGAA	20	7.8572077E-4	44.000004	40
TCGGCAT	50	2.7284841E-11	44.0	32
CGACGGT	195	0.0	42.871796	28
CGGTCTA	195	0.0	42.871796	31
CGTTTTT	7110	0.0	42.390995	1
GCGCGAC	190	0.0	41.68421	9
CGACCCG	165	0.0	41.333332	32
CGCAAGA	80	0.0	41.250004	2
TGCGCGA	70	0.0	40.857143	2
CACGACG	205	0.0	40.780483	26
GAGCGAT	940	0.0	40.723408	7
CGCGCGA	60	3.6379788E-12	40.333336	2
GCCGATT	60	3.6379788E-12	40.333336	9
ACCGCGA	50	1.3478711E-9	39.6	2
TTTCGCG	45	2.3517714E-8	39.11111	1
CCAGTCG	45	2.3517714E-8	39.11111	28
GACGGTC	215	0.0	38.883724	29
CGATGAA	705	0.0	38.695034	19
GCGATAC	120	0.0	38.500004	9
>>END_MODULE