FastQCFastQC Report
Thu 26 May 2016
SRR1546466_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546466_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1034578
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69100.6679051748635675No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64190.620446210918848No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTACAGGTCGTATGCC12610.12188544508002297No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTG502.7284841E-1144.01
CGAACGA207.856117E-444.02
CCGATTC254.4425316E-544.011
CGCGGAT254.4425316E-544.023
ATTACGC207.856117E-444.012
CTAAGCG207.856117E-444.01
AAGCGCG254.4425316E-544.01
TACGCGA254.4425316E-544.02
CTACGCG207.856117E-444.01
GTCCGAA207.856117E-444.040
TCCGTAC207.856117E-444.032
TATAGCG750.044.01
TTACGCG207.856117E-444.01
TATACGA600.044.02
CCACGCG207.856117E-444.01
CGTTTTT93550.043.4355961
CGGTCTA1450.042.4827631
CGACGGT1400.042.4285728
GAGCGAT6550.041.6488537
AGCGATC900.041.5555578
Produced by FastQC (version 0.11.5)