##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546466_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1034578
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.557581931956797	31.0	31.0	34.0	30.0	34.0
2	31.928607606193058	31.0	31.0	34.0	30.0	34.0
3	31.995599171836247	33.0	31.0	34.0	30.0	34.0
4	35.706225146871475	37.0	35.0	37.0	35.0	37.0
5	35.64371366876156	37.0	35.0	37.0	33.0	37.0
6	35.69071737462038	37.0	35.0	37.0	33.0	37.0
7	36.06514443570229	37.0	35.0	37.0	35.0	37.0
8	18.23118991511515	26.0	0.0	37.0	0.0	37.0
9	27.82412249245586	25.0	17.0	39.0	17.0	39.0
10	33.58984629481779	32.0	32.0	39.0	27.0	39.0
11	35.76012345130092	37.0	35.0	39.0	32.0	39.0
12	36.54335970801622	37.0	35.0	39.0	33.0	39.0
13	36.74012109285138	39.0	35.0	39.0	33.0	39.0
14	37.935984527024544	40.0	37.0	41.0	33.0	41.0
15	38.16931637827211	40.0	37.0	41.0	33.0	41.0
16	38.251091749486264	40.0	37.0	41.0	33.0	41.0
17	38.207388906394684	40.0	37.0	41.0	33.0	41.0
18	38.14123729675288	40.0	37.0	41.0	33.0	41.0
19	38.12295158025785	40.0	37.0	41.0	34.0	41.0
20	37.95087755587302	40.0	36.0	41.0	33.0	41.0
21	37.85310919041387	40.0	36.0	41.0	33.0	41.0
22	37.84422537498381	40.0	36.0	41.0	33.0	41.0
23	37.646063419094546	39.0	36.0	41.0	33.0	41.0
24	37.45831150478746	39.0	35.0	41.0	33.0	41.0
25	37.35511193936078	39.0	35.0	41.0	33.0	41.0
26	37.293852179342686	39.0	35.0	41.0	33.0	41.0
27	37.13634158081846	39.0	35.0	41.0	32.0	41.0
28	37.11953859448007	39.0	35.0	41.0	32.0	41.0
29	37.00723483391296	39.0	35.0	41.0	32.0	41.0
30	36.9047640680548	39.0	35.0	41.0	32.0	41.0
31	36.82180753891925	39.0	35.0	41.0	31.0	41.0
32	36.45415522077601	39.0	35.0	41.0	30.0	41.0
33	36.19278971716004	39.0	35.0	41.0	30.0	41.0
34	35.94486544272157	39.0	35.0	41.0	27.0	41.0
35	35.69258383611482	39.0	35.0	41.0	25.0	41.0
36	35.51839590635022	39.0	35.0	41.0	24.0	41.0
37	35.41682405773175	39.0	35.0	41.0	24.0	41.0
38	35.3287263019318	39.0	35.0	41.0	23.0	41.0
39	35.25558053621863	39.0	35.0	41.0	23.0	41.0
40	35.189130254074605	39.0	35.0	41.0	23.0	41.0
41	35.174312618284944	39.0	35.0	40.0	23.0	41.0
42	35.00922018446168	39.0	35.0	40.0	22.0	41.0
43	35.036820810030754	39.0	35.0	40.0	23.0	41.0
44	34.81591238166673	38.0	34.0	40.0	21.0	41.0
45	34.76108422951194	38.0	34.0	40.0	21.0	41.0
46	34.791247252502956	38.0	34.0	40.0	22.0	41.0
47	34.664543417702674	38.0	34.0	40.0	21.0	41.0
48	34.667223737601226	38.0	34.0	40.0	21.0	41.0
49	34.644528493743344	38.0	34.0	40.0	21.0	41.0
50	34.02610532990263	38.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	5.0
15	18.0
16	45.0
17	129.0
18	300.0
19	779.0
20	1572.0
21	2731.0
22	4573.0
23	6977.0
24	10473.0
25	15525.0
26	20375.0
27	21900.0
28	20737.0
29	20325.0
30	22582.0
31	28044.0
32	36183.0
33	47577.0
34	70378.0
35	105764.0
36	100979.0
37	156045.0
38	178118.0
39	162440.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.857605709767654	22.966175580768198	28.02988271546466	18.146335993999486
2	35.0082835706926	24.79716367446437	28.195457471548785	11.999095283294253
3	25.959569988923022	24.738299093930085	36.896976351710556	12.405154565436343
4	23.543802400592316	25.89297278697208	37.01760524581037	13.545619566625234
5	21.79130041427519	29.954822159373194	34.94758249257185	13.306294933779764
6	18.743584340668367	39.272244335371525	31.63338095339356	10.35079037056655
7	82.22975938015307	2.9779291653215125	13.155702131690408	1.6366093228350111
8	42.04158603797877	50.85058835583204	5.997517828525254	1.1103077776639363
9	78.48678398342126	4.698534088294937	13.303008569677685	3.5116733586061177
10	37.756360564404034	33.534929217516705	18.8443017346203	9.864408483458956
11	30.385819145583998	22.732360440682093	33.13795576553918	13.743864648194723
12	28.243496382099753	21.99244522887593	33.0696187237695	16.69443966525482
13	20.598930191827005	30.371513795963185	33.570305960497905	15.459250051711907
14	16.126478622201514	33.25123866929318	34.10752983342	16.5147528750853
15	14.864998095841978	25.29717430681882	43.601739066556604	16.2360885307826
16	17.123793469414583	23.466669501961185	40.90073440571905	18.508802622905186
17	17.71379248350535	24.097361436257103	33.800544763178806	24.38830131705874
18	19.592819487752493	24.75627743872381	36.57645919399021	19.07444387953349
19	22.762807637510175	26.297292229295422	31.334225162336722	19.605674970857685
20	24.709688394688463	25.054369994335858	31.828822959699508	18.40711865127617
21	20.725358551989316	27.330563766095935	33.39429216550129	18.549785516413454
22	20.427265996377265	23.991811153919762	32.96706483223112	22.61385801747186
23	17.959979817858102	27.95864594066373	33.35118280110345	20.730191440374725
24	17.705576573250156	25.43297847044882	38.82491218641804	18.03653276988299
25	17.212911931241532	28.449667400621315	35.4122163819451	18.925204286192052
26	17.535458902083747	30.84426693782392	32.56960809141505	19.050666068677277
27	18.323123051137756	29.03454355302355	33.16801633129643	19.474317064542255
28	16.321727312972055	26.99651452089644	38.49530919853312	18.186448967598384
29	16.787327780022384	24.59785535745009	37.48475223714403	21.13006462538349
30	18.253529458387867	28.219815229011246	34.37971810728626	19.146937205314632
31	22.558569774342775	25.972038840957374	30.82648190856562	20.64290947613423
32	23.40722497482065	27.169048636255553	30.62137412548885	18.802352263434948
33	22.10050861317368	27.58332382865284	29.67925086363715	20.63691669453632
34	18.086601493555825	27.64934108399753	31.730522009940287	22.533535412506357
35	19.58131721339522	27.68307464492769	31.876668554715064	20.858939586962027
36	23.410897969993563	27.578780913570554	29.48806179911036	19.52225931732552
37	19.886369128282254	30.510990954766097	30.349185851622597	19.253454065329052
38	19.968044941995675	30.07970399525217	29.149469638828585	20.802781423923573
39	20.823272870677705	30.219567785125918	28.83755502243427	20.11960432176211
40	22.717571802222743	26.597317940261632	28.878827889245663	21.80628236826996
41	19.509210518684913	26.32667619067871	29.13961054652235	25.024502744114024
42	20.99629027487536	27.408276611333314	28.005331642466786	23.59010147132454
43	21.10870325871998	27.404990247231236	29.37564881526574	22.110657678783042
44	20.354386039525295	29.070306927075578	29.81727815592444	20.75802887747468
45	18.668191281855982	31.346500698835662	27.818202204183734	22.16710581512462
46	20.835645064944355	30.086373381224035	28.53192316094098	20.54605839289063
47	20.49482977600529	27.27952846474601	29.833226687596294	22.3924150716524
48	21.01059562449617	25.2615075905345	31.411841349806398	22.316055435162934
49	20.492026700741754	24.972597522854727	32.21100777321768	22.32436800318584
50	19.04370670940229	28.675169972684518	30.770710376597993	21.510412941315202
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	273.0
1	562.0
2	851.0
3	10954.5
4	21058.0
5	15028.0
6	8998.0
7	9082.0
8	9166.0
9	9488.0
10	9810.0
11	9757.5
12	9705.0
13	9489.0
14	9273.0
15	8775.0
16	8277.0
17	7841.0
18	7405.0
19	7083.0
20	6761.0
21	7054.0
22	7347.0
23	7779.0
24	8211.0
25	9372.0
26	10533.0
27	12354.5
28	14176.0
29	16633.0
30	19090.0
31	22532.5
32	25975.0
33	29720.0
34	33465.0
35	36923.5
36	40382.0
37	44425.5
38	48469.0
39	56275.5
40	64082.0
41	72899.5
42	81717.0
43	87028.5
44	92340.0
45	96637.5
46	100935.0
47	98076.5
48	95218.0
49	88153.5
50	81089.0
51	71415.0
52	61741.0
53	54506.5
54	47272.0
55	41428.0
56	35584.0
57	30981.0
58	26378.0
59	22663.0
60	18948.0
61	16019.5
62	13091.0
63	10468.0
64	7845.0
65	5896.0
66	3947.0
67	3066.0
68	2185.0
69	1771.0
70	1357.0
71	1038.5
72	720.0
73	571.0
74	422.0
75	336.0
76	250.0
77	190.5
78	131.0
79	93.5
80	56.0
81	39.0
82	22.0
83	16.0
84	10.0
85	9.5
86	9.0
87	6.0
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1034578.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.107135932213104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.260169246976	28.580412945409783
2	8.412387933738597	6.747935727459264
3	4.404298029910201	5.299313393147604
4	3.0408171769152172	4.878338714381885
5	2.3133190556282734	4.639030090933101
6	1.8330267967233522	4.4110472942143595
7	1.4871215285687944	4.1750929705820425
8	1.2318322781616697	3.9524211700734257
9	0.9667324630359266	3.4895583274558577
>10	5.009358424477487	30.15026870224239
>50	0.02543557944312881	0.6833864126420038
>100	0.013563800658023855	1.1532910036944126
>500	9.688428816764027E-4	0.32222592781588344
>1k	4.8442144083820135E-4	0.22284783792874183
>5k	4.8442144083820135E-4	1.2948294820192674
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6910	0.6679051748635675	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6419	0.620446210918848	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTACAGGTCGTATGCC	1261	0.12188544508002297	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04117620904368738	0.0
2	0.0	0.0	0.0	0.1444067049560304	0.0
3	0.0	0.0	0.0	0.23603826874339104	0.0
4	0.0	0.0	0.0	0.37251903674735015	0.0
5	0.0	0.0	0.0	0.730346092803056	0.0
6	0.0	0.0	0.0	1.1938200889638093	0.0
7	0.0	0.0	0.0	1.4786705303998344	0.0
8	8.69919909373677E-4	0.0	0.0	2.0534942749604186	0.0
9	8.69919909373677E-4	0.0	0.0	2.657025376530334	0.0
10	8.69919909373677E-4	0.0	0.0	3.4856724190926154	0.0
11	8.69919909373677E-4	0.0	0.0	3.9822033718095686	0.0
12	9.665776770818634E-4	0.0	0.0	4.497582589229618	0.0
13	9.665776770818634E-4	0.0	0.0	4.745413105633408	0.0
14	9.665776770818634E-4	0.0	0.0	4.862465662328022	0.0
15	9.665776770818634E-4	0.0	0.0	4.99469348855282	0.0
16	9.665776770818634E-4	0.0	0.0	5.208017181884788	0.0
17	9.665776770818634E-4	0.0	0.0	5.447438472497965	0.0
18	9.665776770818634E-4	0.0	0.0	5.807585314978668	0.0
19	9.665776770818634E-4	0.0	0.0	6.006120369851282	0.0
20	9.665776770818634E-4	0.0	0.0	6.21944406318325	0.0
21	9.665776770818634E-4	0.0	0.0	6.41981561564232	0.0
22	9.665776770818634E-4	0.0	0.0	6.6438683211898955	0.0
23	9.665776770818634E-4	0.0	0.0	6.898368223565551	0.0
24	9.665776770818634E-4	0.0	0.0	7.095840042993375	0.0
25	9.665776770818634E-4	0.0	0.0	7.259964932561876	0.0
26	9.665776770818634E-4	0.0	0.0	7.420513484725173	0.0
27	9.665776770818634E-4	0.0	0.0	7.593434231155118	0.0
28	9.665776770818634E-4	0.0	0.0	7.775344149981925	0.0
29	9.665776770818634E-4	0.0	0.0	7.9665332145087175	0.0
30	9.665776770818634E-4	0.0	0.0	8.21214060225522	0.0
31	9.665776770818634E-4	0.0	0.0	8.409709079450751	0.0
32	9.665776770818634E-4	0.0	0.0	8.615203493598356	0.0
33	9.665776770818634E-4	0.0	0.0	8.813545232935555	0.0
34	9.665776770818634E-4	0.0	0.0	9.028222135015437	0.0
35	9.665776770818634E-4	0.0	0.0	9.258074306625502	0.0
36	9.665776770818634E-4	0.0	0.0	9.475360968433506	0.0
37	9.665776770818634E-4	0.0	0.0	9.698640411839417	0.0
38	9.665776770818634E-4	0.0	0.0	9.915057153738045	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTG	50	2.7284841E-11	44.0	1
CGAACGA	20	7.856117E-4	44.0	2
CCGATTC	25	4.4425316E-5	44.0	11
CGCGGAT	25	4.4425316E-5	44.0	23
ATTACGC	20	7.856117E-4	44.0	12
CTAAGCG	20	7.856117E-4	44.0	1
AAGCGCG	25	4.4425316E-5	44.0	1
TACGCGA	25	4.4425316E-5	44.0	2
CTACGCG	20	7.856117E-4	44.0	1
GTCCGAA	20	7.856117E-4	44.0	40
TCCGTAC	20	7.856117E-4	44.0	32
TATAGCG	75	0.0	44.0	1
TTACGCG	20	7.856117E-4	44.0	1
TATACGA	60	0.0	44.0	2
CCACGCG	20	7.856117E-4	44.0	1
CGTTTTT	9355	0.0	43.435596	1
CGGTCTA	145	0.0	42.48276	31
CGACGGT	140	0.0	42.42857	28
GAGCGAT	655	0.0	41.648853	7
AGCGATC	90	0.0	41.555557	8
>>END_MODULE