FastQCFastQC Report
Thu 26 May 2016
SRR1546465_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546465_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1285639
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53770.4182356011290883No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50490.39272299611321687No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA15950.12406282012291164No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGA254.4431363E-544.02
TAATGCG502.7284841E-1144.01
CGGCGAA351.4462603E-743.99999631
AAACGCG351.4462603E-743.9999961
TATAGCG700.043.9999961
CGACGGT2350.043.0638328
CGTTTTT70300.042.7795141
TAAGCGA1300.042.307692
AATGCGG1000.041.82
ATTGCGA900.041.5555532
AACGCGA650.040.6153832
GCGTATG1250.040.481
GTAAGCG603.6379788E-1240.3333321
GACCGAT4150.040.2891549
GAGCGAT7650.040.2614367
CGGGTAT557.8216544E-1140.06
TTTGCGA1600.039.8752
CTCACGA2500.039.624
TAGCGCG501.3478711E-939.61
GTTTTTA41450.039.2762342
Produced by FastQC (version 0.11.5)